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Age- and Pregnancy-Associated DNA Methylation Changes in Mammary Epithelial Cells
Postnatal mammary gland development and differentiation occur during puberty and pregnancy. To explore the role of DNA methylation in these processes, we determined the genome-wide DNA methylation and gene expression profiles of CD24(+)CD61(+)CD29(hi), CD24(+)CD61(+)CD29(lo), and CD24(+)CD61(−)CD29(...
Autores principales: | , , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier
2015
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4325231/ https://www.ncbi.nlm.nih.gov/pubmed/25619437 http://dx.doi.org/10.1016/j.stemcr.2014.12.009 |
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author | Huh, Sung Jin Clement, Kendell Jee, David Merlini, Alessandra Choudhury, Sibgat Maruyama, Reo Yoo, Ronnie Chytil, Anna Boyle, Patrick Ran, Fei Ann Moses, Harold L. Barcellos-Hoff, Mary Helen Jackson-Grusby, Laurie Meissner, Alexander Polyak, Kornelia |
author_facet | Huh, Sung Jin Clement, Kendell Jee, David Merlini, Alessandra Choudhury, Sibgat Maruyama, Reo Yoo, Ronnie Chytil, Anna Boyle, Patrick Ran, Fei Ann Moses, Harold L. Barcellos-Hoff, Mary Helen Jackson-Grusby, Laurie Meissner, Alexander Polyak, Kornelia |
author_sort | Huh, Sung Jin |
collection | PubMed |
description | Postnatal mammary gland development and differentiation occur during puberty and pregnancy. To explore the role of DNA methylation in these processes, we determined the genome-wide DNA methylation and gene expression profiles of CD24(+)CD61(+)CD29(hi), CD24(+)CD61(+)CD29(lo), and CD24(+)CD61(−)CD29(lo) cell populations that were previously associated with distinct biological properties at different ages and reproductive stages. We found that pregnancy had the most significant effects on CD24(+)CD61(+)CD29(hi) and CD24(+)CD61(+)CD29(lo) cells, inducing distinct epigenetic states that were maintained through life. Integrated analysis of gene expression, DNA methylation, and histone modification profiles revealed cell-type- and reproductive-stage-specific changes. We identified p27 and TGFβ signaling as key regulators of CD24(+)CD61(+)CD29(lo) cell proliferation, based on their expression patterns and results from mammary gland explant cultures. Our results suggest that relatively minor changes in DNA methylation occur during luminal differentiation compared with the effects of pregnancy on CD24(+)CD61(+)CD29(hi) and CD24(+)CD61(+)CD29(lo) cells. |
format | Online Article Text |
id | pubmed-4325231 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2015 |
publisher | Elsevier |
record_format | MEDLINE/PubMed |
spelling | pubmed-43252312015-02-14 Age- and Pregnancy-Associated DNA Methylation Changes in Mammary Epithelial Cells Huh, Sung Jin Clement, Kendell Jee, David Merlini, Alessandra Choudhury, Sibgat Maruyama, Reo Yoo, Ronnie Chytil, Anna Boyle, Patrick Ran, Fei Ann Moses, Harold L. Barcellos-Hoff, Mary Helen Jackson-Grusby, Laurie Meissner, Alexander Polyak, Kornelia Stem Cell Reports Resource Postnatal mammary gland development and differentiation occur during puberty and pregnancy. To explore the role of DNA methylation in these processes, we determined the genome-wide DNA methylation and gene expression profiles of CD24(+)CD61(+)CD29(hi), CD24(+)CD61(+)CD29(lo), and CD24(+)CD61(−)CD29(lo) cell populations that were previously associated with distinct biological properties at different ages and reproductive stages. We found that pregnancy had the most significant effects on CD24(+)CD61(+)CD29(hi) and CD24(+)CD61(+)CD29(lo) cells, inducing distinct epigenetic states that were maintained through life. Integrated analysis of gene expression, DNA methylation, and histone modification profiles revealed cell-type- and reproductive-stage-specific changes. We identified p27 and TGFβ signaling as key regulators of CD24(+)CD61(+)CD29(lo) cell proliferation, based on their expression patterns and results from mammary gland explant cultures. Our results suggest that relatively minor changes in DNA methylation occur during luminal differentiation compared with the effects of pregnancy on CD24(+)CD61(+)CD29(hi) and CD24(+)CD61(+)CD29(lo) cells. Elsevier 2015-01-22 /pmc/articles/PMC4325231/ /pubmed/25619437 http://dx.doi.org/10.1016/j.stemcr.2014.12.009 Text en © 2015 The Authors http://creativecommons.org/licenses/by-nc-nd/3.0/ This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/3.0/). |
spellingShingle | Resource Huh, Sung Jin Clement, Kendell Jee, David Merlini, Alessandra Choudhury, Sibgat Maruyama, Reo Yoo, Ronnie Chytil, Anna Boyle, Patrick Ran, Fei Ann Moses, Harold L. Barcellos-Hoff, Mary Helen Jackson-Grusby, Laurie Meissner, Alexander Polyak, Kornelia Age- and Pregnancy-Associated DNA Methylation Changes in Mammary Epithelial Cells |
title | Age- and Pregnancy-Associated DNA Methylation Changes in Mammary Epithelial Cells |
title_full | Age- and Pregnancy-Associated DNA Methylation Changes in Mammary Epithelial Cells |
title_fullStr | Age- and Pregnancy-Associated DNA Methylation Changes in Mammary Epithelial Cells |
title_full_unstemmed | Age- and Pregnancy-Associated DNA Methylation Changes in Mammary Epithelial Cells |
title_short | Age- and Pregnancy-Associated DNA Methylation Changes in Mammary Epithelial Cells |
title_sort | age- and pregnancy-associated dna methylation changes in mammary epithelial cells |
topic | Resource |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4325231/ https://www.ncbi.nlm.nih.gov/pubmed/25619437 http://dx.doi.org/10.1016/j.stemcr.2014.12.009 |
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