Microglial Content-Dependent Inhibitory Effects of Calcitonin Gene-Related Peptide (CGRP) on Murine Retroviral Infection of Glial Cells

C57BL/6 (B6) mice develop peripheral neuropathy post-LP-BM5 infection, a murine model of HIV-1 infection, along with the up-regulation of select spinal cord cytokines. We investigated if calcitonin gene-related peptide (CGRP) contributed to the development of peripheral neuropathy by stimulating gli...

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Autores principales: Malon, Jennifer, Grlickova-Duzevik, Eliza, Vaughn, James, Beaulac, Holly, Vunk, Tyler R., Cao, Ling
Formato: Online Artículo Texto
Lenguaje:English
Publicado: 2015
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4325278/
https://www.ncbi.nlm.nih.gov/pubmed/25670002
http://dx.doi.org/10.1016/j.jneuroim.2015.01.010
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author Malon, Jennifer
Grlickova-Duzevik, Eliza
Vaughn, James
Beaulac, Holly
Vunk, Tyler R.
Cao, Ling
author_facet Malon, Jennifer
Grlickova-Duzevik, Eliza
Vaughn, James
Beaulac, Holly
Vunk, Tyler R.
Cao, Ling
author_sort Malon, Jennifer
collection PubMed
description C57BL/6 (B6) mice develop peripheral neuropathy post-LP-BM5 infection, a murine model of HIV-1 infection, along with the up-regulation of select spinal cord cytokines. We investigated if calcitonin gene-related peptide (CGRP) contributed to the development of peripheral neuropathy by stimulating glial responses. An increased expression of lumbar spinal cord CGRP was observed in vivo, post-LP-BM5 infection. Consequently, in vitro CGRP co-treatments led to a microglial content-dependent attenuation of viral loads in spinal cord mixed glia infected with selected doses of LP-BM5. This inhibition was neither caused by the loss of glia nor induced via the direct inhibition of LP-BM5 by CGRP.
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spelling pubmed-43252782016-02-15 Microglial Content-Dependent Inhibitory Effects of Calcitonin Gene-Related Peptide (CGRP) on Murine Retroviral Infection of Glial Cells Malon, Jennifer Grlickova-Duzevik, Eliza Vaughn, James Beaulac, Holly Vunk, Tyler R. Cao, Ling J Neuroimmunol Article C57BL/6 (B6) mice develop peripheral neuropathy post-LP-BM5 infection, a murine model of HIV-1 infection, along with the up-regulation of select spinal cord cytokines. We investigated if calcitonin gene-related peptide (CGRP) contributed to the development of peripheral neuropathy by stimulating glial responses. An increased expression of lumbar spinal cord CGRP was observed in vivo, post-LP-BM5 infection. Consequently, in vitro CGRP co-treatments led to a microglial content-dependent attenuation of viral loads in spinal cord mixed glia infected with selected doses of LP-BM5. This inhibition was neither caused by the loss of glia nor induced via the direct inhibition of LP-BM5 by CGRP. 2015-01-27 2015-02-15 /pmc/articles/PMC4325278/ /pubmed/25670002 http://dx.doi.org/10.1016/j.jneuroim.2015.01.010 Text en © 2015 Published by Elsevier B.V. http://creativecommons.org/licenses/by-nc/4.0/ This manuscript version is made available under the CC BY-NC-ND 4.0 license.
spellingShingle Article
Malon, Jennifer
Grlickova-Duzevik, Eliza
Vaughn, James
Beaulac, Holly
Vunk, Tyler R.
Cao, Ling
Microglial Content-Dependent Inhibitory Effects of Calcitonin Gene-Related Peptide (CGRP) on Murine Retroviral Infection of Glial Cells
title Microglial Content-Dependent Inhibitory Effects of Calcitonin Gene-Related Peptide (CGRP) on Murine Retroviral Infection of Glial Cells
title_full Microglial Content-Dependent Inhibitory Effects of Calcitonin Gene-Related Peptide (CGRP) on Murine Retroviral Infection of Glial Cells
title_fullStr Microglial Content-Dependent Inhibitory Effects of Calcitonin Gene-Related Peptide (CGRP) on Murine Retroviral Infection of Glial Cells
title_full_unstemmed Microglial Content-Dependent Inhibitory Effects of Calcitonin Gene-Related Peptide (CGRP) on Murine Retroviral Infection of Glial Cells
title_short Microglial Content-Dependent Inhibitory Effects of Calcitonin Gene-Related Peptide (CGRP) on Murine Retroviral Infection of Glial Cells
title_sort microglial content-dependent inhibitory effects of calcitonin gene-related peptide (cgrp) on murine retroviral infection of glial cells
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4325278/
https://www.ncbi.nlm.nih.gov/pubmed/25670002
http://dx.doi.org/10.1016/j.jneuroim.2015.01.010
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