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CagA phosphorylation EPIYA-C motifs and the vacA i genotype in Helicobacter pylori strains of asymptomatic children from a high-risk gastric cancer area in northeastern Brazil
Helicobacter pylori infection is one of the most common infections worldwide and is associated with gastric diseases. Virulence factors such as VacA and CagA have been shown to increase the risk of these diseases. Studies have suggested a causal role of CagA EPIYA-C in gastric carcinogenesis and thi...
Autores principales: | , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Instituto Oswaldo Cruz, Ministério da Saúde
2014
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4325609/ https://www.ncbi.nlm.nih.gov/pubmed/25494468 http://dx.doi.org/10.1590/0074-0276140279 |
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author | Braga, Lucia Libanez Bessa Campelo de Oliveira, Maria Aparecida Alves Gonçalves, Maria Helane Rocha Batista Chaves, Fernando Kennedy Benigno, Tiago Gomes da Silva Gomes, Adriana Dias Silva, Cícero Igor Simões Moura Anacleto, Charles Batista, Sérgio de Assis Queiroz, Dulciene Maria Magalhães |
author_facet | Braga, Lucia Libanez Bessa Campelo de Oliveira, Maria Aparecida Alves Gonçalves, Maria Helane Rocha Batista Chaves, Fernando Kennedy Benigno, Tiago Gomes da Silva Gomes, Adriana Dias Silva, Cícero Igor Simões Moura Anacleto, Charles Batista, Sérgio de Assis Queiroz, Dulciene Maria Magalhães |
author_sort | Braga, Lucia Libanez Bessa Campelo |
collection | PubMed |
description | Helicobacter pylori infection is one of the most common infections worldwide and is associated with gastric diseases. Virulence factors such as VacA and CagA have been shown to increase the risk of these diseases. Studies have suggested a causal role of CagA EPIYA-C in gastric carcinogenesis and this factor has been shown to be geographically diverse. We investigated the number of CagA EPIYA motifs and the vacA i genotypes in H. pylori strains from asymptomatic children. We included samples from 40 infected children (18 females and 22 males), extracted DNA directly from the gastric mucus/juice (obtained using the string procedure) and analysed the DNA using polymerase chain reaction and DNA sequencing. The vacA i1 genotype was present in 30 (75%) samples, the i2 allele was present in nine (22.5%) samples and both alleles were present in one (2.5%) sample. The cagA-positive samples showed distinct patterns in the 3’ variable region of cagA and 18 of the 30 (60%) strains contained 1 EPIYA-C motif, whereas 12 (40%) strains contained two EPIYA-C motifs. We confirmed that the studied population was colonised early by the most virulent H. pylori strains, as demonstrated by the high frequency of the vacA i1 allele and the high number of EPIYA-C motifs. Therefore, asymptomatic children from an urban community in Fortaleza in northeastern Brazil are frequently colonised with the most virulent H. pylori strains. |
format | Online Article Text |
id | pubmed-4325609 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2014 |
publisher | Instituto Oswaldo Cruz, Ministério da Saúde |
record_format | MEDLINE/PubMed |
spelling | pubmed-43256092015-02-13 CagA phosphorylation EPIYA-C motifs and the vacA i genotype in Helicobacter pylori strains of asymptomatic children from a high-risk gastric cancer area in northeastern Brazil Braga, Lucia Libanez Bessa Campelo de Oliveira, Maria Aparecida Alves Gonçalves, Maria Helane Rocha Batista Chaves, Fernando Kennedy Benigno, Tiago Gomes da Silva Gomes, Adriana Dias Silva, Cícero Igor Simões Moura Anacleto, Charles Batista, Sérgio de Assis Queiroz, Dulciene Maria Magalhães Mem Inst Oswaldo Cruz Articles Helicobacter pylori infection is one of the most common infections worldwide and is associated with gastric diseases. Virulence factors such as VacA and CagA have been shown to increase the risk of these diseases. Studies have suggested a causal role of CagA EPIYA-C in gastric carcinogenesis and this factor has been shown to be geographically diverse. We investigated the number of CagA EPIYA motifs and the vacA i genotypes in H. pylori strains from asymptomatic children. We included samples from 40 infected children (18 females and 22 males), extracted DNA directly from the gastric mucus/juice (obtained using the string procedure) and analysed the DNA using polymerase chain reaction and DNA sequencing. The vacA i1 genotype was present in 30 (75%) samples, the i2 allele was present in nine (22.5%) samples and both alleles were present in one (2.5%) sample. The cagA-positive samples showed distinct patterns in the 3’ variable region of cagA and 18 of the 30 (60%) strains contained 1 EPIYA-C motif, whereas 12 (40%) strains contained two EPIYA-C motifs. We confirmed that the studied population was colonised early by the most virulent H. pylori strains, as demonstrated by the high frequency of the vacA i1 allele and the high number of EPIYA-C motifs. Therefore, asymptomatic children from an urban community in Fortaleza in northeastern Brazil are frequently colonised with the most virulent H. pylori strains. Instituto Oswaldo Cruz, Ministério da Saúde 2014-12 /pmc/articles/PMC4325609/ /pubmed/25494468 http://dx.doi.org/10.1590/0074-0276140279 Text en http://creativecommons.org/licenses/by-nc/3.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License, which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Articles Braga, Lucia Libanez Bessa Campelo de Oliveira, Maria Aparecida Alves Gonçalves, Maria Helane Rocha Batista Chaves, Fernando Kennedy Benigno, Tiago Gomes da Silva Gomes, Adriana Dias Silva, Cícero Igor Simões Moura Anacleto, Charles Batista, Sérgio de Assis Queiroz, Dulciene Maria Magalhães CagA phosphorylation EPIYA-C motifs and the vacA i genotype in Helicobacter pylori strains of asymptomatic children from a high-risk gastric cancer area in northeastern Brazil |
title | CagA phosphorylation EPIYA-C motifs and the vacA i
genotype in Helicobacter pylori strains of asymptomatic children
from a high-risk gastric cancer area in northeastern Brazil |
title_full | CagA phosphorylation EPIYA-C motifs and the vacA i
genotype in Helicobacter pylori strains of asymptomatic children
from a high-risk gastric cancer area in northeastern Brazil |
title_fullStr | CagA phosphorylation EPIYA-C motifs and the vacA i
genotype in Helicobacter pylori strains of asymptomatic children
from a high-risk gastric cancer area in northeastern Brazil |
title_full_unstemmed | CagA phosphorylation EPIYA-C motifs and the vacA i
genotype in Helicobacter pylori strains of asymptomatic children
from a high-risk gastric cancer area in northeastern Brazil |
title_short | CagA phosphorylation EPIYA-C motifs and the vacA i
genotype in Helicobacter pylori strains of asymptomatic children
from a high-risk gastric cancer area in northeastern Brazil |
title_sort | caga phosphorylation epiya-c motifs and the vaca i
genotype in helicobacter pylori strains of asymptomatic children
from a high-risk gastric cancer area in northeastern brazil |
topic | Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4325609/ https://www.ncbi.nlm.nih.gov/pubmed/25494468 http://dx.doi.org/10.1590/0074-0276140279 |
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