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Advantages and applications of CAR-expressing natural killer cells
In contrast to donor T cells, natural killer (NK) cells are known to mediate anti-cancer effects without the risk of inducing graft-versus-host disease (GvHD). In order to improve cytotoxicity against resistant cancer cells, auspicious efforts have been made with chimeric antigen receptor (CAR) expr...
Autores principales: | , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Frontiers Media S.A.
2015
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4325659/ https://www.ncbi.nlm.nih.gov/pubmed/25729364 http://dx.doi.org/10.3389/fphar.2015.00021 |
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author | Glienke, Wolfgang Esser, Ruth Priesner, Christoph Suerth, Julia D. Schambach, Axel Wels, Winfried S. Grez, Manuel Kloess, Stephan Arseniev, Lubomir Koehl, Ulrike |
author_facet | Glienke, Wolfgang Esser, Ruth Priesner, Christoph Suerth, Julia D. Schambach, Axel Wels, Winfried S. Grez, Manuel Kloess, Stephan Arseniev, Lubomir Koehl, Ulrike |
author_sort | Glienke, Wolfgang |
collection | PubMed |
description | In contrast to donor T cells, natural killer (NK) cells are known to mediate anti-cancer effects without the risk of inducing graft-versus-host disease (GvHD). In order to improve cytotoxicity against resistant cancer cells, auspicious efforts have been made with chimeric antigen receptor (CAR) expressing T- and NK cells. These CAR-modified cells express antigen receptors against tumor-associated surface antigens, thus redirecting the effector cells and enhancing tumor-specific immunosurveillance. However, many cancer antigens are also expressed on healthy tissues, potentially leading to off tumor/on target toxicity by CAR-engineered cells. In order to control such potentially severe side effects, the insertion of suicide genes into CAR-modified effectors can provide a means for efficient depletion of these cells. While CAR-expressing T cells have entered successfully clinical trials, experience with CAR-engineered NK cells is mainly restricted to pre-clinical investigations and predominantly to NK cell lines. In this review we summarize the data on CAR expressing NK cells focusing on the possible advantage using these short-lived effector cells and discuss the necessity of suicide switches. Furthermore, we address the compliance of such modified NK cells with regulatory requirements as a new field in cellular immunotherapy. |
format | Online Article Text |
id | pubmed-4325659 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2015 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-43256592015-02-27 Advantages and applications of CAR-expressing natural killer cells Glienke, Wolfgang Esser, Ruth Priesner, Christoph Suerth, Julia D. Schambach, Axel Wels, Winfried S. Grez, Manuel Kloess, Stephan Arseniev, Lubomir Koehl, Ulrike Front Pharmacol Pharmacology In contrast to donor T cells, natural killer (NK) cells are known to mediate anti-cancer effects without the risk of inducing graft-versus-host disease (GvHD). In order to improve cytotoxicity against resistant cancer cells, auspicious efforts have been made with chimeric antigen receptor (CAR) expressing T- and NK cells. These CAR-modified cells express antigen receptors against tumor-associated surface antigens, thus redirecting the effector cells and enhancing tumor-specific immunosurveillance. However, many cancer antigens are also expressed on healthy tissues, potentially leading to off tumor/on target toxicity by CAR-engineered cells. In order to control such potentially severe side effects, the insertion of suicide genes into CAR-modified effectors can provide a means for efficient depletion of these cells. While CAR-expressing T cells have entered successfully clinical trials, experience with CAR-engineered NK cells is mainly restricted to pre-clinical investigations and predominantly to NK cell lines. In this review we summarize the data on CAR expressing NK cells focusing on the possible advantage using these short-lived effector cells and discuss the necessity of suicide switches. Furthermore, we address the compliance of such modified NK cells with regulatory requirements as a new field in cellular immunotherapy. Frontiers Media S.A. 2015-02-12 /pmc/articles/PMC4325659/ /pubmed/25729364 http://dx.doi.org/10.3389/fphar.2015.00021 Text en Copyright © 2015 Glienke, Esser, Priesner, Suerth, Schambach, Wels, Grez, Kloess, Arseniev and Koehl. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Pharmacology Glienke, Wolfgang Esser, Ruth Priesner, Christoph Suerth, Julia D. Schambach, Axel Wels, Winfried S. Grez, Manuel Kloess, Stephan Arseniev, Lubomir Koehl, Ulrike Advantages and applications of CAR-expressing natural killer cells |
title | Advantages and applications of CAR-expressing natural killer cells |
title_full | Advantages and applications of CAR-expressing natural killer cells |
title_fullStr | Advantages and applications of CAR-expressing natural killer cells |
title_full_unstemmed | Advantages and applications of CAR-expressing natural killer cells |
title_short | Advantages and applications of CAR-expressing natural killer cells |
title_sort | advantages and applications of car-expressing natural killer cells |
topic | Pharmacology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4325659/ https://www.ncbi.nlm.nih.gov/pubmed/25729364 http://dx.doi.org/10.3389/fphar.2015.00021 |
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