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Blood Vessel Matrix Seeded with Cells: A Better Alternative for Abdominal Wall Reconstruction—A Long-Term Study

Purpose. The aim of this study was to present abdominal wall reconstruction using a porcine vascular graft seeded with MSC (mesenchymal stem cells) on rat model. Material and Methods. Abdominal wall defect was prepared in 21 Wistar rats. Acellular porcine-vascular grafts taken from aorta and prepare...

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Autores principales: Nowacki, Maciej, Jundziłł, Arkadiusz, Nazarewski, Łukasz, Kotela, Andrzej, Kloskowski, Tomasz, Skopińska-Wisniewska, Joanna, Bodnar, Magdalena, Łukasiewicz, Aleksander, Nazarewski, Sławomir, Kotela, Ireneusz, Kucharzewski, Marek, Pokrywczyńska, Marta, Marszałek, Andrzej, Drewa, Tomasz
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi Publishing Corporation 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4326343/
https://www.ncbi.nlm.nih.gov/pubmed/25705696
http://dx.doi.org/10.1155/2015/890613
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author Nowacki, Maciej
Jundziłł, Arkadiusz
Nazarewski, Łukasz
Kotela, Andrzej
Kloskowski, Tomasz
Skopińska-Wisniewska, Joanna
Bodnar, Magdalena
Łukasiewicz, Aleksander
Nazarewski, Sławomir
Kotela, Ireneusz
Kucharzewski, Marek
Pokrywczyńska, Marta
Marszałek, Andrzej
Drewa, Tomasz
author_facet Nowacki, Maciej
Jundziłł, Arkadiusz
Nazarewski, Łukasz
Kotela, Andrzej
Kloskowski, Tomasz
Skopińska-Wisniewska, Joanna
Bodnar, Magdalena
Łukasiewicz, Aleksander
Nazarewski, Sławomir
Kotela, Ireneusz
Kucharzewski, Marek
Pokrywczyńska, Marta
Marszałek, Andrzej
Drewa, Tomasz
author_sort Nowacki, Maciej
collection PubMed
description Purpose. The aim of this study was to present abdominal wall reconstruction using a porcine vascular graft seeded with MSC (mesenchymal stem cells) on rat model. Material and Methods. Abdominal wall defect was prepared in 21 Wistar rats. Acellular porcine-vascular grafts taken from aorta and prepared with Triton X were used. 14 aortic grafts were implanted in place, of which 7 grafts were seeded with rat MSC cells (Group I), and 7 were acellular grafts (Group II). As a control, 7 standard polypropylene meshes were used for defect augmentation (Group III). The assessment method was performed by HE and CD31 staining after 6 months. The mechanical properties have been investigated by Zwick&Roell Z0.5. Results. The strongest angiogenesis and lowest inflammatory response were observed in Group I. Average capillaries density was 2.75, 0.75, and 1.53 and inflammatory effect was 0.29, 1.39, and 2.72 for Groups I, II, and III, respectively. The means of mechanical properties were 12.74 ± 1.48, 7.27 ± 1.56, and 14.4 ± 3.7 N/cm in Groups I and II and control, respectively. Conclusions. Cell-seeded grafts have better mechanical properties than acellular grafts but worse than polypropylene mesh. Cells improved mechanical and physiological properties of decellularized natural scaffolds.
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spelling pubmed-43263432015-02-22 Blood Vessel Matrix Seeded with Cells: A Better Alternative for Abdominal Wall Reconstruction—A Long-Term Study Nowacki, Maciej Jundziłł, Arkadiusz Nazarewski, Łukasz Kotela, Andrzej Kloskowski, Tomasz Skopińska-Wisniewska, Joanna Bodnar, Magdalena Łukasiewicz, Aleksander Nazarewski, Sławomir Kotela, Ireneusz Kucharzewski, Marek Pokrywczyńska, Marta Marszałek, Andrzej Drewa, Tomasz Biomed Res Int Research Article Purpose. The aim of this study was to present abdominal wall reconstruction using a porcine vascular graft seeded with MSC (mesenchymal stem cells) on rat model. Material and Methods. Abdominal wall defect was prepared in 21 Wistar rats. Acellular porcine-vascular grafts taken from aorta and prepared with Triton X were used. 14 aortic grafts were implanted in place, of which 7 grafts were seeded with rat MSC cells (Group I), and 7 were acellular grafts (Group II). As a control, 7 standard polypropylene meshes were used for defect augmentation (Group III). The assessment method was performed by HE and CD31 staining after 6 months. The mechanical properties have been investigated by Zwick&Roell Z0.5. Results. The strongest angiogenesis and lowest inflammatory response were observed in Group I. Average capillaries density was 2.75, 0.75, and 1.53 and inflammatory effect was 0.29, 1.39, and 2.72 for Groups I, II, and III, respectively. The means of mechanical properties were 12.74 ± 1.48, 7.27 ± 1.56, and 14.4 ± 3.7 N/cm in Groups I and II and control, respectively. Conclusions. Cell-seeded grafts have better mechanical properties than acellular grafts but worse than polypropylene mesh. Cells improved mechanical and physiological properties of decellularized natural scaffolds. Hindawi Publishing Corporation 2015 2015-02-05 /pmc/articles/PMC4326343/ /pubmed/25705696 http://dx.doi.org/10.1155/2015/890613 Text en Copyright © 2015 Maciej Nowacki et al. https://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Nowacki, Maciej
Jundziłł, Arkadiusz
Nazarewski, Łukasz
Kotela, Andrzej
Kloskowski, Tomasz
Skopińska-Wisniewska, Joanna
Bodnar, Magdalena
Łukasiewicz, Aleksander
Nazarewski, Sławomir
Kotela, Ireneusz
Kucharzewski, Marek
Pokrywczyńska, Marta
Marszałek, Andrzej
Drewa, Tomasz
Blood Vessel Matrix Seeded with Cells: A Better Alternative for Abdominal Wall Reconstruction—A Long-Term Study
title Blood Vessel Matrix Seeded with Cells: A Better Alternative for Abdominal Wall Reconstruction—A Long-Term Study
title_full Blood Vessel Matrix Seeded with Cells: A Better Alternative for Abdominal Wall Reconstruction—A Long-Term Study
title_fullStr Blood Vessel Matrix Seeded with Cells: A Better Alternative for Abdominal Wall Reconstruction—A Long-Term Study
title_full_unstemmed Blood Vessel Matrix Seeded with Cells: A Better Alternative for Abdominal Wall Reconstruction—A Long-Term Study
title_short Blood Vessel Matrix Seeded with Cells: A Better Alternative for Abdominal Wall Reconstruction—A Long-Term Study
title_sort blood vessel matrix seeded with cells: a better alternative for abdominal wall reconstruction—a long-term study
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4326343/
https://www.ncbi.nlm.nih.gov/pubmed/25705696
http://dx.doi.org/10.1155/2015/890613
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