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Fluralaner activity against life stages of ticks using Rhipicephalus sanguineus and Ornithodoros moubata IN in vitro contact and feeding assays
BACKGROUND: Fluralaner is a novel isoxazoline eliciting both acaricidal and insecticidal activity through potent blockage of GABA- and glutamate-gated chloride channels. The aim of the study was to investigate the susceptibility of juvenile stages of common tick species exposed to fluralaner through...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2015
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4326483/ https://www.ncbi.nlm.nih.gov/pubmed/25888750 http://dx.doi.org/10.1186/s13071-015-0704-x |
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author | Williams, Heike Zoller, Hartmut Roepke, Rainer KA Zschiesche, Eva Heckeroth, Anja R |
author_facet | Williams, Heike Zoller, Hartmut Roepke, Rainer KA Zschiesche, Eva Heckeroth, Anja R |
author_sort | Williams, Heike |
collection | PubMed |
description | BACKGROUND: Fluralaner is a novel isoxazoline eliciting both acaricidal and insecticidal activity through potent blockage of GABA- and glutamate-gated chloride channels. The aim of the study was to investigate the susceptibility of juvenile stages of common tick species exposed to fluralaner through either contact (Rhipicephalus sanguineus) or contact and feeding routes (Ornithodoros moubata). METHODS: Fluralaner acaricidal activity through both contact and feeding exposure was measured in vitro using two separate testing protocols. Acaricidal contact activity against Rhipicephalus sanguineus life stages was assessed using three minute immersion in fluralaner concentrations between 50 and 0.05 μg/mL (larvae) or between 1000 and 0.2 μg/mL (nymphs and adults). Contact and feeding activity against Ornithodoros moubata nymphs was assessed using fluralaner concentrations between 1000 to 10(−4) μg/mL (contact test) and 0.1 to 10(−10) μg/mL (feeding test). Activity was assessed 48 hours after exposure and all tests included vehicle and untreated negative control groups. RESULTS: Fluralaner lethal concentrations (LC(50), LC(90/95)) were defined as concentrations with either 50%, 90% or 95% killing effect in the tested sample population. After contact exposure of R. sanguineus life stages lethal concentrations were (μg/mL): larvae - LC(50) 0.7, LC(90) 2.4; nymphs - LC(50) 1.4, LC(90) 2.6; and adults - LC(50) 278, LC(90) 1973. After exposure of O. moubata nymphs to fluralaner lethal concentrations were (μg/mL): contact exposure - LC(50) 720(,) LC(95) 1133; and feeding exposure- LC(50) 0.00007, LC(95) 0.09. CONCLUSIONS: Fluralaner demonstrates potent in vitro acaricidal activity against all life stages of the brown dog tick, R.sanguineus. The testing of fluralaner contact and feeding routes using O. moubata nymphs demonstrates a high acaricidal activity in both exposure routes. |
format | Online Article Text |
id | pubmed-4326483 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2015 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-43264832015-02-14 Fluralaner activity against life stages of ticks using Rhipicephalus sanguineus and Ornithodoros moubata IN in vitro contact and feeding assays Williams, Heike Zoller, Hartmut Roepke, Rainer KA Zschiesche, Eva Heckeroth, Anja R Parasit Vectors Research BACKGROUND: Fluralaner is a novel isoxazoline eliciting both acaricidal and insecticidal activity through potent blockage of GABA- and glutamate-gated chloride channels. The aim of the study was to investigate the susceptibility of juvenile stages of common tick species exposed to fluralaner through either contact (Rhipicephalus sanguineus) or contact and feeding routes (Ornithodoros moubata). METHODS: Fluralaner acaricidal activity through both contact and feeding exposure was measured in vitro using two separate testing protocols. Acaricidal contact activity against Rhipicephalus sanguineus life stages was assessed using three minute immersion in fluralaner concentrations between 50 and 0.05 μg/mL (larvae) or between 1000 and 0.2 μg/mL (nymphs and adults). Contact and feeding activity against Ornithodoros moubata nymphs was assessed using fluralaner concentrations between 1000 to 10(−4) μg/mL (contact test) and 0.1 to 10(−10) μg/mL (feeding test). Activity was assessed 48 hours after exposure and all tests included vehicle and untreated negative control groups. RESULTS: Fluralaner lethal concentrations (LC(50), LC(90/95)) were defined as concentrations with either 50%, 90% or 95% killing effect in the tested sample population. After contact exposure of R. sanguineus life stages lethal concentrations were (μg/mL): larvae - LC(50) 0.7, LC(90) 2.4; nymphs - LC(50) 1.4, LC(90) 2.6; and adults - LC(50) 278, LC(90) 1973. After exposure of O. moubata nymphs to fluralaner lethal concentrations were (μg/mL): contact exposure - LC(50) 720(,) LC(95) 1133; and feeding exposure- LC(50) 0.00007, LC(95) 0.09. CONCLUSIONS: Fluralaner demonstrates potent in vitro acaricidal activity against all life stages of the brown dog tick, R.sanguineus. The testing of fluralaner contact and feeding routes using O. moubata nymphs demonstrates a high acaricidal activity in both exposure routes. BioMed Central 2015-02-08 /pmc/articles/PMC4326483/ /pubmed/25888750 http://dx.doi.org/10.1186/s13071-015-0704-x Text en © Williams et al.; licensee BioMed Central. 2015 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated. |
spellingShingle | Research Williams, Heike Zoller, Hartmut Roepke, Rainer KA Zschiesche, Eva Heckeroth, Anja R Fluralaner activity against life stages of ticks using Rhipicephalus sanguineus and Ornithodoros moubata IN in vitro contact and feeding assays |
title | Fluralaner activity against life stages of ticks using Rhipicephalus sanguineus and Ornithodoros moubata IN in vitro contact and feeding assays |
title_full | Fluralaner activity against life stages of ticks using Rhipicephalus sanguineus and Ornithodoros moubata IN in vitro contact and feeding assays |
title_fullStr | Fluralaner activity against life stages of ticks using Rhipicephalus sanguineus and Ornithodoros moubata IN in vitro contact and feeding assays |
title_full_unstemmed | Fluralaner activity against life stages of ticks using Rhipicephalus sanguineus and Ornithodoros moubata IN in vitro contact and feeding assays |
title_short | Fluralaner activity against life stages of ticks using Rhipicephalus sanguineus and Ornithodoros moubata IN in vitro contact and feeding assays |
title_sort | fluralaner activity against life stages of ticks using rhipicephalus sanguineus and ornithodoros moubata in in vitro contact and feeding assays |
topic | Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4326483/ https://www.ncbi.nlm.nih.gov/pubmed/25888750 http://dx.doi.org/10.1186/s13071-015-0704-x |
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