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The effect of skin fatty acids on Staphylococcus aureus
Staphylococcus aureus is a commensal of the human nose and skin. Human skin fatty acids, in particular cis-6-hexadecenoic acid (C-6-H), have high antistaphylococcal activity and can inhibit virulence determinant production. Here, we show that sub-MIC levels of C-6-H result in induction of increased...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Springer Berlin Heidelberg
2014
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4326651/ https://www.ncbi.nlm.nih.gov/pubmed/25325933 http://dx.doi.org/10.1007/s00203-014-1048-1 |
_version_ | 1782356956411854848 |
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author | Neumann, Yvonne Ohlsen, Knut Donat, Stefanie Engelmann, Susanne Kusch, Harald Albrecht, Dirk Cartron, Michael Hurd, Alexander Foster, Simon J. |
author_facet | Neumann, Yvonne Ohlsen, Knut Donat, Stefanie Engelmann, Susanne Kusch, Harald Albrecht, Dirk Cartron, Michael Hurd, Alexander Foster, Simon J. |
author_sort | Neumann, Yvonne |
collection | PubMed |
description | Staphylococcus aureus is a commensal of the human nose and skin. Human skin fatty acids, in particular cis-6-hexadecenoic acid (C-6-H), have high antistaphylococcal activity and can inhibit virulence determinant production. Here, we show that sub-MIC levels of C-6-H result in induction of increased resistance. The mechanism(s) of C-6-H activity was investigated by combined transcriptome and proteome analyses. Proteome analysis demonstrated a pleiotropic effect of C-6-H on virulence determinant production. In response to C-6-H, transcriptomics revealed altered expression of over 500 genes, involved in many aspects of virulence and cellular physiology. The expression of toxins (hla, hlb, hlgBC) was reduced, whereas that of host defence evasion components (cap, sspAB, katA) was increased. In particular, members of the SaeRS regulon had highly reduced expression, and the use of specific mutants revealed that the effect on toxin production is likely mediated via SaeRS. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1007/s00203-014-1048-1) contains supplementary material, which is available to authorized users. |
format | Online Article Text |
id | pubmed-4326651 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2014 |
publisher | Springer Berlin Heidelberg |
record_format | MEDLINE/PubMed |
spelling | pubmed-43266512015-02-19 The effect of skin fatty acids on Staphylococcus aureus Neumann, Yvonne Ohlsen, Knut Donat, Stefanie Engelmann, Susanne Kusch, Harald Albrecht, Dirk Cartron, Michael Hurd, Alexander Foster, Simon J. Arch Microbiol Orginal Paper Staphylococcus aureus is a commensal of the human nose and skin. Human skin fatty acids, in particular cis-6-hexadecenoic acid (C-6-H), have high antistaphylococcal activity and can inhibit virulence determinant production. Here, we show that sub-MIC levels of C-6-H result in induction of increased resistance. The mechanism(s) of C-6-H activity was investigated by combined transcriptome and proteome analyses. Proteome analysis demonstrated a pleiotropic effect of C-6-H on virulence determinant production. In response to C-6-H, transcriptomics revealed altered expression of over 500 genes, involved in many aspects of virulence and cellular physiology. The expression of toxins (hla, hlb, hlgBC) was reduced, whereas that of host defence evasion components (cap, sspAB, katA) was increased. In particular, members of the SaeRS regulon had highly reduced expression, and the use of specific mutants revealed that the effect on toxin production is likely mediated via SaeRS. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1007/s00203-014-1048-1) contains supplementary material, which is available to authorized users. Springer Berlin Heidelberg 2014-10-18 2015 /pmc/articles/PMC4326651/ /pubmed/25325933 http://dx.doi.org/10.1007/s00203-014-1048-1 Text en © The Author(s) 2014 https://creativecommons.org/licenses/by/4.0/ Open AccessThis article is distributed under the terms of the Creative Commons Attribution License which permits any use, distribution, and reproduction in any medium, provided the original author(s) and the source are credited. |
spellingShingle | Orginal Paper Neumann, Yvonne Ohlsen, Knut Donat, Stefanie Engelmann, Susanne Kusch, Harald Albrecht, Dirk Cartron, Michael Hurd, Alexander Foster, Simon J. The effect of skin fatty acids on Staphylococcus aureus |
title | The effect of skin fatty acids on Staphylococcus aureus |
title_full | The effect of skin fatty acids on Staphylococcus aureus |
title_fullStr | The effect of skin fatty acids on Staphylococcus aureus |
title_full_unstemmed | The effect of skin fatty acids on Staphylococcus aureus |
title_short | The effect of skin fatty acids on Staphylococcus aureus |
title_sort | effect of skin fatty acids on staphylococcus aureus |
topic | Orginal Paper |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4326651/ https://www.ncbi.nlm.nih.gov/pubmed/25325933 http://dx.doi.org/10.1007/s00203-014-1048-1 |
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