Cargando…

Terahertz in-line digital holography of human hepatocellular carcinoma tissue

Terahertz waves provide a better contrast in imaging soft biomedical tissues than X-rays, and unlike X-rays, they cause no ionisation damage, making them a good option for biomedical imaging. Terahertz absorption imaging has conventionally been used for cancer diagnosis. However, the absorption prop...

Descripción completa

Detalles Bibliográficos
Autores principales: Rong, Lu, Latychevskaia, Tatiana, Chen, Chunhai, Wang, Dayong, Yu, Zhengping, Zhou, Xun, Li, Zeyu, Huang, Haochong, Wang, Yunxin, Zhou, Zhou
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4326957/
https://www.ncbi.nlm.nih.gov/pubmed/25676705
http://dx.doi.org/10.1038/srep08445
_version_ 1782356981043953664
author Rong, Lu
Latychevskaia, Tatiana
Chen, Chunhai
Wang, Dayong
Yu, Zhengping
Zhou, Xun
Li, Zeyu
Huang, Haochong
Wang, Yunxin
Zhou, Zhou
author_facet Rong, Lu
Latychevskaia, Tatiana
Chen, Chunhai
Wang, Dayong
Yu, Zhengping
Zhou, Xun
Li, Zeyu
Huang, Haochong
Wang, Yunxin
Zhou, Zhou
author_sort Rong, Lu
collection PubMed
description Terahertz waves provide a better contrast in imaging soft biomedical tissues than X-rays, and unlike X-rays, they cause no ionisation damage, making them a good option for biomedical imaging. Terahertz absorption imaging has conventionally been used for cancer diagnosis. However, the absorption properties of a cancerous sample are influenced by two opposing factors: an increase in absorption due to a higher degree of hydration and a decrease in absorption due to structural changes. It is therefore difficult to diagnose cancer from an absorption image. Phase imaging can thus be critical for diagnostics. We demonstrate imaging of the absorption and phase-shift distributions of 3.2 mm × 2.3 mm × 30-μm-thick human hepatocellular carcinoma tissue by continuous-wave terahertz digital in-line holography. The acquisition time of a few seconds for a single in-line hologram is much shorter than that of other terahertz diagnostic techniques, and future detectors will allow acquisition of meaningful holograms without sample dehydration. The resolution of the reconstructions was enhanced by sub-pixel shifting and extrapolation. Another advantage of this technique is its relaxed minimal sample size limitation. The fibrosis indicated in the phase distribution demonstrates the potential of terahertz holographic imaging to obtain a more objective, early diagnosis of cancer.
format Online
Article
Text
id pubmed-4326957
institution National Center for Biotechnology Information
language English
publishDate 2015
publisher Nature Publishing Group
record_format MEDLINE/PubMed
spelling pubmed-43269572015-04-08 Terahertz in-line digital holography of human hepatocellular carcinoma tissue Rong, Lu Latychevskaia, Tatiana Chen, Chunhai Wang, Dayong Yu, Zhengping Zhou, Xun Li, Zeyu Huang, Haochong Wang, Yunxin Zhou, Zhou Sci Rep Article Terahertz waves provide a better contrast in imaging soft biomedical tissues than X-rays, and unlike X-rays, they cause no ionisation damage, making them a good option for biomedical imaging. Terahertz absorption imaging has conventionally been used for cancer diagnosis. However, the absorption properties of a cancerous sample are influenced by two opposing factors: an increase in absorption due to a higher degree of hydration and a decrease in absorption due to structural changes. It is therefore difficult to diagnose cancer from an absorption image. Phase imaging can thus be critical for diagnostics. We demonstrate imaging of the absorption and phase-shift distributions of 3.2 mm × 2.3 mm × 30-μm-thick human hepatocellular carcinoma tissue by continuous-wave terahertz digital in-line holography. The acquisition time of a few seconds for a single in-line hologram is much shorter than that of other terahertz diagnostic techniques, and future detectors will allow acquisition of meaningful holograms without sample dehydration. The resolution of the reconstructions was enhanced by sub-pixel shifting and extrapolation. Another advantage of this technique is its relaxed minimal sample size limitation. The fibrosis indicated in the phase distribution demonstrates the potential of terahertz holographic imaging to obtain a more objective, early diagnosis of cancer. Nature Publishing Group 2015-02-13 /pmc/articles/PMC4326957/ /pubmed/25676705 http://dx.doi.org/10.1038/srep08445 Text en Copyright © 2015, Macmillan Publishers Limited. All rights reserved http://creativecommons.org/licenses/by/4.0/ This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article's Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder in order to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/
spellingShingle Article
Rong, Lu
Latychevskaia, Tatiana
Chen, Chunhai
Wang, Dayong
Yu, Zhengping
Zhou, Xun
Li, Zeyu
Huang, Haochong
Wang, Yunxin
Zhou, Zhou
Terahertz in-line digital holography of human hepatocellular carcinoma tissue
title Terahertz in-line digital holography of human hepatocellular carcinoma tissue
title_full Terahertz in-line digital holography of human hepatocellular carcinoma tissue
title_fullStr Terahertz in-line digital holography of human hepatocellular carcinoma tissue
title_full_unstemmed Terahertz in-line digital holography of human hepatocellular carcinoma tissue
title_short Terahertz in-line digital holography of human hepatocellular carcinoma tissue
title_sort terahertz in-line digital holography of human hepatocellular carcinoma tissue
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4326957/
https://www.ncbi.nlm.nih.gov/pubmed/25676705
http://dx.doi.org/10.1038/srep08445
work_keys_str_mv AT ronglu terahertzinlinedigitalholographyofhumanhepatocellularcarcinomatissue
AT latychevskaiatatiana terahertzinlinedigitalholographyofhumanhepatocellularcarcinomatissue
AT chenchunhai terahertzinlinedigitalholographyofhumanhepatocellularcarcinomatissue
AT wangdayong terahertzinlinedigitalholographyofhumanhepatocellularcarcinomatissue
AT yuzhengping terahertzinlinedigitalholographyofhumanhepatocellularcarcinomatissue
AT zhouxun terahertzinlinedigitalholographyofhumanhepatocellularcarcinomatissue
AT lizeyu terahertzinlinedigitalholographyofhumanhepatocellularcarcinomatissue
AT huanghaochong terahertzinlinedigitalholographyofhumanhepatocellularcarcinomatissue
AT wangyunxin terahertzinlinedigitalholographyofhumanhepatocellularcarcinomatissue
AT zhouzhou terahertzinlinedigitalholographyofhumanhepatocellularcarcinomatissue