Imaging of Fluoride Ion in Living Cells and Tissues with a Two-Photon Ratiometric Fluorescence Probe

A reaction-based two-photon (TP) ratiometric fluorescence probe Z2 has been developed and successfully applied to detect and image fluoride ion in living cells and tissues. The Z2 probe was designed designed to utilize an ICT mechanism between n-butylnaphthalimide as a fluorophore and tert-butyldiph...

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Autores principales: Zhu, Xinyue, Wang, Jianxi, Zhang, Jianjian, Chen, Zhenjie, Zhang, Haixia, Zhang, Xiaoyu
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2015
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4327094/
https://www.ncbi.nlm.nih.gov/pubmed/25594597
http://dx.doi.org/10.3390/s150101611
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author Zhu, Xinyue
Wang, Jianxi
Zhang, Jianjian
Chen, Zhenjie
Zhang, Haixia
Zhang, Xiaoyu
author_facet Zhu, Xinyue
Wang, Jianxi
Zhang, Jianjian
Chen, Zhenjie
Zhang, Haixia
Zhang, Xiaoyu
author_sort Zhu, Xinyue
collection PubMed
description A reaction-based two-photon (TP) ratiometric fluorescence probe Z2 has been developed and successfully applied to detect and image fluoride ion in living cells and tissues. The Z2 probe was designed designed to utilize an ICT mechanism between n-butylnaphthalimide as a fluorophore and tert-butyldiphenylsilane (TBDPS) as a response group. Upon addition of fluoride ion, the Si-O bond in the Z2 would be cleaved, and then a stronger electron-donating group was released. The fluorescent changes at 450 and 540 nm, respectively, made it possible to achieve ratiometric fluorescence detection. The results indicated that the Z2 could ratiometrically detect and image fluoride ion in living cells and tissues in a depth of 250 μm by two-photon microscopy (TPM).
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spelling pubmed-43270942015-02-23 Imaging of Fluoride Ion in Living Cells and Tissues with a Two-Photon Ratiometric Fluorescence Probe Zhu, Xinyue Wang, Jianxi Zhang, Jianjian Chen, Zhenjie Zhang, Haixia Zhang, Xiaoyu Sensors (Basel) Article A reaction-based two-photon (TP) ratiometric fluorescence probe Z2 has been developed and successfully applied to detect and image fluoride ion in living cells and tissues. The Z2 probe was designed designed to utilize an ICT mechanism between n-butylnaphthalimide as a fluorophore and tert-butyldiphenylsilane (TBDPS) as a response group. Upon addition of fluoride ion, the Si-O bond in the Z2 would be cleaved, and then a stronger electron-donating group was released. The fluorescent changes at 450 and 540 nm, respectively, made it possible to achieve ratiometric fluorescence detection. The results indicated that the Z2 could ratiometrically detect and image fluoride ion in living cells and tissues in a depth of 250 μm by two-photon microscopy (TPM). MDPI 2015-01-14 /pmc/articles/PMC4327094/ /pubmed/25594597 http://dx.doi.org/10.3390/s150101611 Text en © 2015 by the authors; licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Zhu, Xinyue
Wang, Jianxi
Zhang, Jianjian
Chen, Zhenjie
Zhang, Haixia
Zhang, Xiaoyu
Imaging of Fluoride Ion in Living Cells and Tissues with a Two-Photon Ratiometric Fluorescence Probe
title Imaging of Fluoride Ion in Living Cells and Tissues with a Two-Photon Ratiometric Fluorescence Probe
title_full Imaging of Fluoride Ion in Living Cells and Tissues with a Two-Photon Ratiometric Fluorescence Probe
title_fullStr Imaging of Fluoride Ion in Living Cells and Tissues with a Two-Photon Ratiometric Fluorescence Probe
title_full_unstemmed Imaging of Fluoride Ion in Living Cells and Tissues with a Two-Photon Ratiometric Fluorescence Probe
title_short Imaging of Fluoride Ion in Living Cells and Tissues with a Two-Photon Ratiometric Fluorescence Probe
title_sort imaging of fluoride ion in living cells and tissues with a two-photon ratiometric fluorescence probe
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4327094/
https://www.ncbi.nlm.nih.gov/pubmed/25594597
http://dx.doi.org/10.3390/s150101611
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