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Effect of Ozone on Intestinal Epithelial Homeostasis in a Rat Model

BACKGROUND: The positive effects of ozone therapy have been described in many gastrointestinal disorders. The mechanisms of this positive effect of ozone therapy are poorly understood. The purpose of the present study was to investigate whether the use of ozone may potentiate the gut intestinal muco...

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Autores principales: Sukhotnik, Igor, Starikov, Alona, Coran, Arnold G., Pollak, Yulia, Sohotnik, Rima, Shaoul, Ron
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Rambam Health Care Campus 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4327322/
https://www.ncbi.nlm.nih.gov/pubmed/25717388
http://dx.doi.org/10.5041/RMMJ.10181
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author Sukhotnik, Igor
Starikov, Alona
Coran, Arnold G.
Pollak, Yulia
Sohotnik, Rima
Shaoul, Ron
author_facet Sukhotnik, Igor
Starikov, Alona
Coran, Arnold G.
Pollak, Yulia
Sohotnik, Rima
Shaoul, Ron
author_sort Sukhotnik, Igor
collection PubMed
description BACKGROUND: The positive effects of ozone therapy have been described in many gastrointestinal disorders. The mechanisms of this positive effect of ozone therapy are poorly understood. The purpose of the present study was to investigate whether the use of ozone may potentiate the gut intestinal mucosal homeostasis in a rat model. METHODS: Adult rats weighing 250–280 g were randomly assigned to one of three experimental groups of 8 rats each: 1) Control rats were given 2 mL of water by gavage and intraperitoneally (IP) for 5 days; 2) O(3)-PO rats were treated with 2 mL of ozone/oxygen mixture by gavage and 2 mL of water IP for 5 days; 3) O(3)-IP rats were treated with 2 mL of water by gavage and 2 mL of ozone/oxygen mixture IP for 5 days. Rats were sacrificed on day 6. Bowel and mucosal weight, mucosal DNA and protein, villus height and crypt depth, and cell proliferation and apoptosis were evaluated following sacrifice. RESULTS: The group of O(3)-IP rats demonstrated a greater jejunal and ileal villus height and crypt depth, a greater enterocyte proliferation index in jejunum, and lower enterocyte apoptosis in ileum compared to control animals. Oral administration of the ozone/oxygen mixture resulted in a less significant effect on cell turnover. CONCLUSIONS: Treatment with an ozone/oxygen mixture stimulates intestinal cell turnover in a rat model. Intraperitoneal administration of ozone resulted in a more significant intestinal trophic effect than oral administration.
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spelling pubmed-43273222015-02-25 Effect of Ozone on Intestinal Epithelial Homeostasis in a Rat Model Sukhotnik, Igor Starikov, Alona Coran, Arnold G. Pollak, Yulia Sohotnik, Rima Shaoul, Ron Rambam Maimonides Med J Basic Research BACKGROUND: The positive effects of ozone therapy have been described in many gastrointestinal disorders. The mechanisms of this positive effect of ozone therapy are poorly understood. The purpose of the present study was to investigate whether the use of ozone may potentiate the gut intestinal mucosal homeostasis in a rat model. METHODS: Adult rats weighing 250–280 g were randomly assigned to one of three experimental groups of 8 rats each: 1) Control rats were given 2 mL of water by gavage and intraperitoneally (IP) for 5 days; 2) O(3)-PO rats were treated with 2 mL of ozone/oxygen mixture by gavage and 2 mL of water IP for 5 days; 3) O(3)-IP rats were treated with 2 mL of water by gavage and 2 mL of ozone/oxygen mixture IP for 5 days. Rats were sacrificed on day 6. Bowel and mucosal weight, mucosal DNA and protein, villus height and crypt depth, and cell proliferation and apoptosis were evaluated following sacrifice. RESULTS: The group of O(3)-IP rats demonstrated a greater jejunal and ileal villus height and crypt depth, a greater enterocyte proliferation index in jejunum, and lower enterocyte apoptosis in ileum compared to control animals. Oral administration of the ozone/oxygen mixture resulted in a less significant effect on cell turnover. CONCLUSIONS: Treatment with an ozone/oxygen mixture stimulates intestinal cell turnover in a rat model. Intraperitoneal administration of ozone resulted in a more significant intestinal trophic effect than oral administration. Rambam Health Care Campus 2015-01-29 /pmc/articles/PMC4327322/ /pubmed/25717388 http://dx.doi.org/10.5041/RMMJ.10181 Text en Copyright: © 2015 Sukhotnik et al. This is an open-access article. All its content, except where otherwise noted, is distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Basic Research
Sukhotnik, Igor
Starikov, Alona
Coran, Arnold G.
Pollak, Yulia
Sohotnik, Rima
Shaoul, Ron
Effect of Ozone on Intestinal Epithelial Homeostasis in a Rat Model
title Effect of Ozone on Intestinal Epithelial Homeostasis in a Rat Model
title_full Effect of Ozone on Intestinal Epithelial Homeostasis in a Rat Model
title_fullStr Effect of Ozone on Intestinal Epithelial Homeostasis in a Rat Model
title_full_unstemmed Effect of Ozone on Intestinal Epithelial Homeostasis in a Rat Model
title_short Effect of Ozone on Intestinal Epithelial Homeostasis in a Rat Model
title_sort effect of ozone on intestinal epithelial homeostasis in a rat model
topic Basic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4327322/
https://www.ncbi.nlm.nih.gov/pubmed/25717388
http://dx.doi.org/10.5041/RMMJ.10181
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