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Effects of homogenization process parameters on physicochemical properties of astaxanthin nanodispersions prepared using a solvent-diffusion technique

Nanodispersion systems allow incorporation of lipophilic bioactives, such as astaxanthin (a fat soluble carotenoid) into aqueous systems, which can improve their solubility, bioavailability, and stability, and widen their uses in water-based pharmaceutical and food products. In this study, response...

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Autores principales: Anarjan, Navideh, Jafarizadeh-Malmiri, Hoda, Nehdi, Imededdine Arbi, Sbihi, Hassen Mohamed, Al-Resayes, Saud Ibrahim, Tan, Chin Ping
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Dove Medical Press 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4327565/
https://www.ncbi.nlm.nih.gov/pubmed/25709435
http://dx.doi.org/10.2147/IJN.S72835
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author Anarjan, Navideh
Jafarizadeh-Malmiri, Hoda
Nehdi, Imededdine Arbi
Sbihi, Hassen Mohamed
Al-Resayes, Saud Ibrahim
Tan, Chin Ping
author_facet Anarjan, Navideh
Jafarizadeh-Malmiri, Hoda
Nehdi, Imededdine Arbi
Sbihi, Hassen Mohamed
Al-Resayes, Saud Ibrahim
Tan, Chin Ping
author_sort Anarjan, Navideh
collection PubMed
description Nanodispersion systems allow incorporation of lipophilic bioactives, such as astaxanthin (a fat soluble carotenoid) into aqueous systems, which can improve their solubility, bioavailability, and stability, and widen their uses in water-based pharmaceutical and food products. In this study, response surface methodology was used to investigate the influences of homogenization time (0.5–20 minutes) and speed (1,000–9,000 rpm) in the formation of astaxanthin nanodispersions via the solvent-diffusion process. The product was characterized for particle size and astaxanthin concentration using laser diffraction particle size analysis and high performance liquid chromatography, respectively. Relatively high determination coefficients (ranging from 0.896 to 0.969) were obtained for all suggested polynomial regression models. The overall optimal homogenization conditions were determined by multiple response optimization analysis to be 6,000 rpm for 7 minutes. In vitro cellular uptake of astaxanthin from the suggested individual and multiple optimized astaxanthin nanodispersions was also evaluated. The cellular uptake of astaxanthin was found to be considerably increased (by more than five times) as it became incorporated into optimum nanodispersion systems. The lack of a significant difference between predicted and experimental values confirms the suitability of the regression equations connecting the response variables studied to the independent parameters.
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spelling pubmed-43275652015-02-23 Effects of homogenization process parameters on physicochemical properties of astaxanthin nanodispersions prepared using a solvent-diffusion technique Anarjan, Navideh Jafarizadeh-Malmiri, Hoda Nehdi, Imededdine Arbi Sbihi, Hassen Mohamed Al-Resayes, Saud Ibrahim Tan, Chin Ping Int J Nanomedicine Original Research Nanodispersion systems allow incorporation of lipophilic bioactives, such as astaxanthin (a fat soluble carotenoid) into aqueous systems, which can improve their solubility, bioavailability, and stability, and widen their uses in water-based pharmaceutical and food products. In this study, response surface methodology was used to investigate the influences of homogenization time (0.5–20 minutes) and speed (1,000–9,000 rpm) in the formation of astaxanthin nanodispersions via the solvent-diffusion process. The product was characterized for particle size and astaxanthin concentration using laser diffraction particle size analysis and high performance liquid chromatography, respectively. Relatively high determination coefficients (ranging from 0.896 to 0.969) were obtained for all suggested polynomial regression models. The overall optimal homogenization conditions were determined by multiple response optimization analysis to be 6,000 rpm for 7 minutes. In vitro cellular uptake of astaxanthin from the suggested individual and multiple optimized astaxanthin nanodispersions was also evaluated. The cellular uptake of astaxanthin was found to be considerably increased (by more than five times) as it became incorporated into optimum nanodispersion systems. The lack of a significant difference between predicted and experimental values confirms the suitability of the regression equations connecting the response variables studied to the independent parameters. Dove Medical Press 2015-02-04 /pmc/articles/PMC4327565/ /pubmed/25709435 http://dx.doi.org/10.2147/IJN.S72835 Text en © 2015 Anarjan et al. This work is published by Dove Medical Press Limited, and licensed under Creative Commons Attribution – Non Commercial (unported, v3.0) License The full terms of the License are available at http://creativecommons.org/licenses/by-nc/3.0/. Non-commercial uses of the work are permitted without any further permission from Dove Medical Press Limited, provided the work is properly attributed.
spellingShingle Original Research
Anarjan, Navideh
Jafarizadeh-Malmiri, Hoda
Nehdi, Imededdine Arbi
Sbihi, Hassen Mohamed
Al-Resayes, Saud Ibrahim
Tan, Chin Ping
Effects of homogenization process parameters on physicochemical properties of astaxanthin nanodispersions prepared using a solvent-diffusion technique
title Effects of homogenization process parameters on physicochemical properties of astaxanthin nanodispersions prepared using a solvent-diffusion technique
title_full Effects of homogenization process parameters on physicochemical properties of astaxanthin nanodispersions prepared using a solvent-diffusion technique
title_fullStr Effects of homogenization process parameters on physicochemical properties of astaxanthin nanodispersions prepared using a solvent-diffusion technique
title_full_unstemmed Effects of homogenization process parameters on physicochemical properties of astaxanthin nanodispersions prepared using a solvent-diffusion technique
title_short Effects of homogenization process parameters on physicochemical properties of astaxanthin nanodispersions prepared using a solvent-diffusion technique
title_sort effects of homogenization process parameters on physicochemical properties of astaxanthin nanodispersions prepared using a solvent-diffusion technique
topic Original Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4327565/
https://www.ncbi.nlm.nih.gov/pubmed/25709435
http://dx.doi.org/10.2147/IJN.S72835
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