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Sustained-release nanoART formulation for the treatment of neuroAIDS
A novel approach was developed for the coencapsulation of an anti-HIV drug (tenofovir) and a latency-breaking agent (vorinostat), using magnetically guided layer-by-layer (LbL) assembled nanocarriers for the treatment of neuroAIDS. Ultrasmall iron oxide (Fe(3)O(4)) nanoparticles (10±3 nm) were synth...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Dove Medical Press
2015
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4327567/ https://www.ncbi.nlm.nih.gov/pubmed/25709433 http://dx.doi.org/10.2147/IJN.S76517 |
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author | Jayant, Rahul Dev Atluri, Venkata SR Agudelo, Marisela Sagar, Vidya Kaushik, Ajeet Nair, Madhavan |
author_facet | Jayant, Rahul Dev Atluri, Venkata SR Agudelo, Marisela Sagar, Vidya Kaushik, Ajeet Nair, Madhavan |
author_sort | Jayant, Rahul Dev |
collection | PubMed |
description | A novel approach was developed for the coencapsulation of an anti-HIV drug (tenofovir) and a latency-breaking agent (vorinostat), using magnetically guided layer-by-layer (LbL) assembled nanocarriers for the treatment of neuroAIDS. Ultrasmall iron oxide (Fe(3)O(4)) nanoparticles (10±3 nm) were synthesized and characterized. The LbL technique was used to achieve a sustained release profile, and application of 2 bilayers ([tenofovir+dextran sulphate](2)+vorinostat) to magnetic nanoparticles resulted in a 2.8 times increase in drug (tenofovir) loading and also resulted in an increase in the drug release period by 30-fold, with 100% drug release in sustained manner over a period of 5 days with the simultaneous stimulation of latent HIV expression. Nanoformulation showed a good blood–brain barrier transmigration ability (37.95%±1.5%) with good in vitro antiviral efficacy (~33% reduction of p24 level) over a period of 5 days after HIV infection in primary human astrocytes, with good cell viability (>90%). Hence, LbL arrangements of drugs on magnetic nanoparticles provides sustained release and, therefore, may improve the patient’s adherence to therapy and lead to better compliance. |
format | Online Article Text |
id | pubmed-4327567 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2015 |
publisher | Dove Medical Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-43275672015-02-23 Sustained-release nanoART formulation for the treatment of neuroAIDS Jayant, Rahul Dev Atluri, Venkata SR Agudelo, Marisela Sagar, Vidya Kaushik, Ajeet Nair, Madhavan Int J Nanomedicine Original Research A novel approach was developed for the coencapsulation of an anti-HIV drug (tenofovir) and a latency-breaking agent (vorinostat), using magnetically guided layer-by-layer (LbL) assembled nanocarriers for the treatment of neuroAIDS. Ultrasmall iron oxide (Fe(3)O(4)) nanoparticles (10±3 nm) were synthesized and characterized. The LbL technique was used to achieve a sustained release profile, and application of 2 bilayers ([tenofovir+dextran sulphate](2)+vorinostat) to magnetic nanoparticles resulted in a 2.8 times increase in drug (tenofovir) loading and also resulted in an increase in the drug release period by 30-fold, with 100% drug release in sustained manner over a period of 5 days with the simultaneous stimulation of latent HIV expression. Nanoformulation showed a good blood–brain barrier transmigration ability (37.95%±1.5%) with good in vitro antiviral efficacy (~33% reduction of p24 level) over a period of 5 days after HIV infection in primary human astrocytes, with good cell viability (>90%). Hence, LbL arrangements of drugs on magnetic nanoparticles provides sustained release and, therefore, may improve the patient’s adherence to therapy and lead to better compliance. Dove Medical Press 2015-02-04 /pmc/articles/PMC4327567/ /pubmed/25709433 http://dx.doi.org/10.2147/IJN.S76517 Text en © 2015 Jayant et al. This work is published by Dove Medical Press Limited, and licensed under Creative Commons Attribution – Non Commercial (unported, v3.0) License The full terms of the License are available at http://creativecommons.org/licenses/by-nc/3.0/. Non-commercial uses of the work are permitted without any further permission from Dove Medical Press Limited, provided the work is properly attributed. |
spellingShingle | Original Research Jayant, Rahul Dev Atluri, Venkata SR Agudelo, Marisela Sagar, Vidya Kaushik, Ajeet Nair, Madhavan Sustained-release nanoART formulation for the treatment of neuroAIDS |
title | Sustained-release nanoART formulation for the treatment of neuroAIDS |
title_full | Sustained-release nanoART formulation for the treatment of neuroAIDS |
title_fullStr | Sustained-release nanoART formulation for the treatment of neuroAIDS |
title_full_unstemmed | Sustained-release nanoART formulation for the treatment of neuroAIDS |
title_short | Sustained-release nanoART formulation for the treatment of neuroAIDS |
title_sort | sustained-release nanoart formulation for the treatment of neuroaids |
topic | Original Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4327567/ https://www.ncbi.nlm.nih.gov/pubmed/25709433 http://dx.doi.org/10.2147/IJN.S76517 |
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