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Titanium dioxide nanoparticles enhance production of superoxide anion and alter the antioxidant system in human osteoblast cells
Titanium dioxide (TiO(2)) nanoparticles (NPs) are manufactured worldwide for a variety of engineering and bioengineering applications. TiO(2)NPs are frequently used as a material for orthopedic implants. However, to the best of our knowledge, the biocompatibility of TiO(2)NPs and their effects on os...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Dove Medical Press
2015
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4327568/ https://www.ncbi.nlm.nih.gov/pubmed/25709434 http://dx.doi.org/10.2147/IJN.S73557 |
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author | Niska, Karolina Pyszka, Katarzyna Tukaj, Cecylia Wozniak, Michal Radomski, Marek Witold Inkielewicz-Stepniak, Iwona |
author_facet | Niska, Karolina Pyszka, Katarzyna Tukaj, Cecylia Wozniak, Michal Radomski, Marek Witold Inkielewicz-Stepniak, Iwona |
author_sort | Niska, Karolina |
collection | PubMed |
description | Titanium dioxide (TiO(2)) nanoparticles (NPs) are manufactured worldwide for a variety of engineering and bioengineering applications. TiO(2)NPs are frequently used as a material for orthopedic implants. However, to the best of our knowledge, the biocompatibility of TiO(2)NPs and their effects on osteoblast cells, which are responsible for the growth and remodeling of the human skeleton, have not been thoroughly investigated. In the research reported here, we studied the effects of exposing hFOB 1.19 human osteoblast cells to TiO(2)NPs (5–15 nm) for 24 and 48 hours. Cell viability, alkaline phosphatase (ALP) activity, cellular uptake of NPs, cell morphology, superoxide anion (O(2)(•−2)) generation, superoxide dismutase (SOD) activity and protein level, sirtuin 3 (SIR3) protein level, correlation between manganese (Mn) SOD and SIR, total antioxidant capacity, and malondialdehyde were measured following exposure of hFOB 1.19 cells to TiO(2)NPs. Exposure of hFOB 1.19 cells to TiO(2)NPs resulted in: (1) cellular uptake of NPs; (2) increased cytotoxicity and cell death in a time- and concentration-dependent manner; (3) ultrastructure changes; (4) decreased SOD and ALP activity; (5) decreased protein levels of SOD1, SOD2, and SIR3; (6) decreased total antioxidant capacity; (7) increased O(2)(•−) generation; and (8) enhanced lipid peroxidation (malondialdehyde level). The linear relationship between the protein level of MnSOD and SIR3 and between O(2)(•−) content and SIR3 protein level was observed. Importantly, the cytotoxic effects of TiO(2)NPs were attenuated by the pretreatment of hFOB 1.19 cells with SOD, indicating the significant role of O(2)(•−) in the cell damage and death observed. Thus, decreased expression of SOD leading to increased oxidizing stress may underlie the nanotoxic effects of TiO(2)NPs on human osteoblasts. |
format | Online Article Text |
id | pubmed-4327568 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2015 |
publisher | Dove Medical Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-43275682015-02-23 Titanium dioxide nanoparticles enhance production of superoxide anion and alter the antioxidant system in human osteoblast cells Niska, Karolina Pyszka, Katarzyna Tukaj, Cecylia Wozniak, Michal Radomski, Marek Witold Inkielewicz-Stepniak, Iwona Int J Nanomedicine Original Research Titanium dioxide (TiO(2)) nanoparticles (NPs) are manufactured worldwide for a variety of engineering and bioengineering applications. TiO(2)NPs are frequently used as a material for orthopedic implants. However, to the best of our knowledge, the biocompatibility of TiO(2)NPs and their effects on osteoblast cells, which are responsible for the growth and remodeling of the human skeleton, have not been thoroughly investigated. In the research reported here, we studied the effects of exposing hFOB 1.19 human osteoblast cells to TiO(2)NPs (5–15 nm) for 24 and 48 hours. Cell viability, alkaline phosphatase (ALP) activity, cellular uptake of NPs, cell morphology, superoxide anion (O(2)(•−2)) generation, superoxide dismutase (SOD) activity and protein level, sirtuin 3 (SIR3) protein level, correlation between manganese (Mn) SOD and SIR, total antioxidant capacity, and malondialdehyde were measured following exposure of hFOB 1.19 cells to TiO(2)NPs. Exposure of hFOB 1.19 cells to TiO(2)NPs resulted in: (1) cellular uptake of NPs; (2) increased cytotoxicity and cell death in a time- and concentration-dependent manner; (3) ultrastructure changes; (4) decreased SOD and ALP activity; (5) decreased protein levels of SOD1, SOD2, and SIR3; (6) decreased total antioxidant capacity; (7) increased O(2)(•−) generation; and (8) enhanced lipid peroxidation (malondialdehyde level). The linear relationship between the protein level of MnSOD and SIR3 and between O(2)(•−) content and SIR3 protein level was observed. Importantly, the cytotoxic effects of TiO(2)NPs were attenuated by the pretreatment of hFOB 1.19 cells with SOD, indicating the significant role of O(2)(•−) in the cell damage and death observed. Thus, decreased expression of SOD leading to increased oxidizing stress may underlie the nanotoxic effects of TiO(2)NPs on human osteoblasts. Dove Medical Press 2015-02-04 /pmc/articles/PMC4327568/ /pubmed/25709434 http://dx.doi.org/10.2147/IJN.S73557 Text en © 2015 Niska et al. This work is published by Dove Medical Press Limited, and licensed under Creative Commons Attribution – Non Commercial (unported, v3.0) License The full terms of the License are available at http://creativecommons.org/licenses/by-nc/3.0/. Non-commercial uses of the work are permitted without any further permission from Dove Medical Press Limited, provided the work is properly attributed. |
spellingShingle | Original Research Niska, Karolina Pyszka, Katarzyna Tukaj, Cecylia Wozniak, Michal Radomski, Marek Witold Inkielewicz-Stepniak, Iwona Titanium dioxide nanoparticles enhance production of superoxide anion and alter the antioxidant system in human osteoblast cells |
title | Titanium dioxide nanoparticles enhance production of superoxide anion and alter the antioxidant system in human osteoblast cells |
title_full | Titanium dioxide nanoparticles enhance production of superoxide anion and alter the antioxidant system in human osteoblast cells |
title_fullStr | Titanium dioxide nanoparticles enhance production of superoxide anion and alter the antioxidant system in human osteoblast cells |
title_full_unstemmed | Titanium dioxide nanoparticles enhance production of superoxide anion and alter the antioxidant system in human osteoblast cells |
title_short | Titanium dioxide nanoparticles enhance production of superoxide anion and alter the antioxidant system in human osteoblast cells |
title_sort | titanium dioxide nanoparticles enhance production of superoxide anion and alter the antioxidant system in human osteoblast cells |
topic | Original Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4327568/ https://www.ncbi.nlm.nih.gov/pubmed/25709434 http://dx.doi.org/10.2147/IJN.S73557 |
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