A MODEL SYSTEM TO STUDY ANTIMICROBIAL STRATEGIES IN ENDODONTIC BIOFILMS

The purpose of this work was to develop a model system to study antimicrobial strategies in endodontic biofilms. Enterococcus faecalis suspension was colonized in 10 human root canals. Five milliliters of Brain Heart Infusion (BHI) were mixed with 5 mL of the bacterial inoculums (E. faecalis) and in...

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Detalles Bibliográficos
Autores principales: Estrela, Carlos, Sydney, Gilson Blitzkow, Figueiredo, José Antonio Poli, Estrela, Cyntia Rodrigues de Araújo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Faculdade de Odontologia de Bauru da Universidade de São Paulo 2009
Materias:
Original Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4327582/
https://www.ncbi.nlm.nih.gov/pubmed/19274391
http://dx.doi.org/10.1590/S1678-77572009000200003
Descripción
Sumario:The purpose of this work was to develop a model system to study antimicrobial strategies in endodontic biofilms. Enterococcus faecalis suspension was colonized in 10 human root canals. Five milliliters of Brain Heart Infusion (BHI) were mixed with 5 mL of the bacterial inoculums (E. faecalis) and inoculated with sufficient volume to fill the root canal during 60 days. This procedure was repeated every 72 h, always using 24-h pure culture prepared and adjusted to No. 1 MacFarland turbidity standard. Biofilm formation was analyzed by scanning electron microscopy (SEM). E. faecalis consistently adhered to collagen structure, colonized dentin surface, progressed towards the dentinal tubules and formed a biofilm. The proposed biofilm model seems to be viable for studies on antimicrobial strategies, and allows for a satisfactory colonization time of selected bacterial species with virulence and adherence properties.

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