Cargando…

Anti-fibrotic effect of Holothuria arenicola extract against bile duct ligation in rats

BACKGROUND: Holothuria arenicola is the most important and abundant sea cucumber species in the Mediterranean Sea on the Egyptian coast. The present study aims to assess the anti-oxidative and anticholestatic effects of the sea cucumber Holothuria arenicola extract (HaE) in a model of bile duct liga...

Descripción completa

Detalles Bibliográficos
Autor principal: Fahmy, Sohair R
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4328034/
https://www.ncbi.nlm.nih.gov/pubmed/25652675
http://dx.doi.org/10.1186/s12906-015-0533-7
_version_ 1782357171577552896
author Fahmy, Sohair R
author_facet Fahmy, Sohair R
author_sort Fahmy, Sohair R
collection PubMed
description BACKGROUND: Holothuria arenicola is the most important and abundant sea cucumber species in the Mediterranean Sea on the Egyptian coast. The present study aims to assess the anti-oxidative and anticholestatic effects of the sea cucumber Holothuria arenicola extract (HaE) in a model of bile duct ligation in male albino rats. METHODS: Fifty four male Wistar albino rats were assigned into two main groups, the Sham-operated control and bile duct ligated (BDL) group. After 14 days of surgery, the animals of the group I (Sham control) received distilled water only for 7, 14 and 28 days. Second group (BDL group) was divided into 2 subgroups, animals of these subgroups treated for 7, 14 and 28 consecutive days as follow: subgroup I (BDL), rats of this subgroup administered distilled water orally. Subgroup II (HaE), animals of this subgroup treated orally with HaE (200 mg/kg body weight). RESULTS: The HaE revealed significant antifibrotic effect as evident by decreasing the levels of total conjugated and unconjugated bilirubin and the activities of serum aminotransferases (ASAT and ALAT) and alkaline phosphatase (ALP) as well as malondialdehyde (MDA) level, and increasing the serum albumin, glutathione reduced (GSH) levels. Treatment with HaE normalized the antioxidant enzyme, glutathione-S-transferase (GST), superoxide dismutase (SOD) and catalase (CAT) activities activities. CONCLUSION: The present prospective study correlated the antifibrotic effect of HaE to its direct antioxidant effect that can be related to its contents of phenolic compounds specially chlorogenic acid, pyrogallol, rutin and coumaric acid.
format Online
Article
Text
id pubmed-4328034
institution National Center for Biotechnology Information
language English
publishDate 2015
publisher BioMed Central
record_format MEDLINE/PubMed
spelling pubmed-43280342015-02-15 Anti-fibrotic effect of Holothuria arenicola extract against bile duct ligation in rats Fahmy, Sohair R BMC Complement Altern Med Research Article BACKGROUND: Holothuria arenicola is the most important and abundant sea cucumber species in the Mediterranean Sea on the Egyptian coast. The present study aims to assess the anti-oxidative and anticholestatic effects of the sea cucumber Holothuria arenicola extract (HaE) in a model of bile duct ligation in male albino rats. METHODS: Fifty four male Wistar albino rats were assigned into two main groups, the Sham-operated control and bile duct ligated (BDL) group. After 14 days of surgery, the animals of the group I (Sham control) received distilled water only for 7, 14 and 28 days. Second group (BDL group) was divided into 2 subgroups, animals of these subgroups treated for 7, 14 and 28 consecutive days as follow: subgroup I (BDL), rats of this subgroup administered distilled water orally. Subgroup II (HaE), animals of this subgroup treated orally with HaE (200 mg/kg body weight). RESULTS: The HaE revealed significant antifibrotic effect as evident by decreasing the levels of total conjugated and unconjugated bilirubin and the activities of serum aminotransferases (ASAT and ALAT) and alkaline phosphatase (ALP) as well as malondialdehyde (MDA) level, and increasing the serum albumin, glutathione reduced (GSH) levels. Treatment with HaE normalized the antioxidant enzyme, glutathione-S-transferase (GST), superoxide dismutase (SOD) and catalase (CAT) activities activities. CONCLUSION: The present prospective study correlated the antifibrotic effect of HaE to its direct antioxidant effect that can be related to its contents of phenolic compounds specially chlorogenic acid, pyrogallol, rutin and coumaric acid. BioMed Central 2015-02-05 /pmc/articles/PMC4328034/ /pubmed/25652675 http://dx.doi.org/10.1186/s12906-015-0533-7 Text en © Fahmy; licensee BioMed Central. 2015 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Research Article
Fahmy, Sohair R
Anti-fibrotic effect of Holothuria arenicola extract against bile duct ligation in rats
title Anti-fibrotic effect of Holothuria arenicola extract against bile duct ligation in rats
title_full Anti-fibrotic effect of Holothuria arenicola extract against bile duct ligation in rats
title_fullStr Anti-fibrotic effect of Holothuria arenicola extract against bile duct ligation in rats
title_full_unstemmed Anti-fibrotic effect of Holothuria arenicola extract against bile duct ligation in rats
title_short Anti-fibrotic effect of Holothuria arenicola extract against bile duct ligation in rats
title_sort anti-fibrotic effect of holothuria arenicola extract against bile duct ligation in rats
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4328034/
https://www.ncbi.nlm.nih.gov/pubmed/25652675
http://dx.doi.org/10.1186/s12906-015-0533-7
work_keys_str_mv AT fahmysohairr antifibroticeffectofholothuriaarenicolaextractagainstbileductligationinrats