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Anti-fibrotic effect of Holothuria arenicola extract against bile duct ligation in rats
BACKGROUND: Holothuria arenicola is the most important and abundant sea cucumber species in the Mediterranean Sea on the Egyptian coast. The present study aims to assess the anti-oxidative and anticholestatic effects of the sea cucumber Holothuria arenicola extract (HaE) in a model of bile duct liga...
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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BioMed Central
2015
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4328034/ https://www.ncbi.nlm.nih.gov/pubmed/25652675 http://dx.doi.org/10.1186/s12906-015-0533-7 |
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author | Fahmy, Sohair R |
author_facet | Fahmy, Sohair R |
author_sort | Fahmy, Sohair R |
collection | PubMed |
description | BACKGROUND: Holothuria arenicola is the most important and abundant sea cucumber species in the Mediterranean Sea on the Egyptian coast. The present study aims to assess the anti-oxidative and anticholestatic effects of the sea cucumber Holothuria arenicola extract (HaE) in a model of bile duct ligation in male albino rats. METHODS: Fifty four male Wistar albino rats were assigned into two main groups, the Sham-operated control and bile duct ligated (BDL) group. After 14 days of surgery, the animals of the group I (Sham control) received distilled water only for 7, 14 and 28 days. Second group (BDL group) was divided into 2 subgroups, animals of these subgroups treated for 7, 14 and 28 consecutive days as follow: subgroup I (BDL), rats of this subgroup administered distilled water orally. Subgroup II (HaE), animals of this subgroup treated orally with HaE (200 mg/kg body weight). RESULTS: The HaE revealed significant antifibrotic effect as evident by decreasing the levels of total conjugated and unconjugated bilirubin and the activities of serum aminotransferases (ASAT and ALAT) and alkaline phosphatase (ALP) as well as malondialdehyde (MDA) level, and increasing the serum albumin, glutathione reduced (GSH) levels. Treatment with HaE normalized the antioxidant enzyme, glutathione-S-transferase (GST), superoxide dismutase (SOD) and catalase (CAT) activities activities. CONCLUSION: The present prospective study correlated the antifibrotic effect of HaE to its direct antioxidant effect that can be related to its contents of phenolic compounds specially chlorogenic acid, pyrogallol, rutin and coumaric acid. |
format | Online Article Text |
id | pubmed-4328034 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2015 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-43280342015-02-15 Anti-fibrotic effect of Holothuria arenicola extract against bile duct ligation in rats Fahmy, Sohair R BMC Complement Altern Med Research Article BACKGROUND: Holothuria arenicola is the most important and abundant sea cucumber species in the Mediterranean Sea on the Egyptian coast. The present study aims to assess the anti-oxidative and anticholestatic effects of the sea cucumber Holothuria arenicola extract (HaE) in a model of bile duct ligation in male albino rats. METHODS: Fifty four male Wistar albino rats were assigned into two main groups, the Sham-operated control and bile duct ligated (BDL) group. After 14 days of surgery, the animals of the group I (Sham control) received distilled water only for 7, 14 and 28 days. Second group (BDL group) was divided into 2 subgroups, animals of these subgroups treated for 7, 14 and 28 consecutive days as follow: subgroup I (BDL), rats of this subgroup administered distilled water orally. Subgroup II (HaE), animals of this subgroup treated orally with HaE (200 mg/kg body weight). RESULTS: The HaE revealed significant antifibrotic effect as evident by decreasing the levels of total conjugated and unconjugated bilirubin and the activities of serum aminotransferases (ASAT and ALAT) and alkaline phosphatase (ALP) as well as malondialdehyde (MDA) level, and increasing the serum albumin, glutathione reduced (GSH) levels. Treatment with HaE normalized the antioxidant enzyme, glutathione-S-transferase (GST), superoxide dismutase (SOD) and catalase (CAT) activities activities. CONCLUSION: The present prospective study correlated the antifibrotic effect of HaE to its direct antioxidant effect that can be related to its contents of phenolic compounds specially chlorogenic acid, pyrogallol, rutin and coumaric acid. BioMed Central 2015-02-05 /pmc/articles/PMC4328034/ /pubmed/25652675 http://dx.doi.org/10.1186/s12906-015-0533-7 Text en © Fahmy; licensee BioMed Central. 2015 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated. |
spellingShingle | Research Article Fahmy, Sohair R Anti-fibrotic effect of Holothuria arenicola extract against bile duct ligation in rats |
title | Anti-fibrotic effect of Holothuria arenicola extract against bile duct ligation in rats |
title_full | Anti-fibrotic effect of Holothuria arenicola extract against bile duct ligation in rats |
title_fullStr | Anti-fibrotic effect of Holothuria arenicola extract against bile duct ligation in rats |
title_full_unstemmed | Anti-fibrotic effect of Holothuria arenicola extract against bile duct ligation in rats |
title_short | Anti-fibrotic effect of Holothuria arenicola extract against bile duct ligation in rats |
title_sort | anti-fibrotic effect of holothuria arenicola extract against bile duct ligation in rats |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4328034/ https://www.ncbi.nlm.nih.gov/pubmed/25652675 http://dx.doi.org/10.1186/s12906-015-0533-7 |
work_keys_str_mv | AT fahmysohairr antifibroticeffectofholothuriaarenicolaextractagainstbileductligationinrats |