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Repetitive sequences based on genotyping of Candida albicans isolates obtained from Iranian patients with human immunodeficiency virus

OBJECTIVE(S): Candidiasis infection caused by Candida albicans has been known as a major problem in patients with immune disorders. The objective of this study was to genotype the C. albicans isolates obtained from oral cavity of patients with positive human immunodeficiency virus (HIV(+)) with or/a...

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Autores principales: Tamai, Iradj Ashrafi, Salehi, Taghi Zahraei, Sharifzadeh, Aghil, Shokri, Hojjatollah, Khosravi, Ali Reza
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Mashhad University of Medical Sciences 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4328090/
https://www.ncbi.nlm.nih.gov/pubmed/25691923
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author Tamai, Iradj Ashrafi
Salehi, Taghi Zahraei
Sharifzadeh, Aghil
Shokri, Hojjatollah
Khosravi, Ali Reza
author_facet Tamai, Iradj Ashrafi
Salehi, Taghi Zahraei
Sharifzadeh, Aghil
Shokri, Hojjatollah
Khosravi, Ali Reza
author_sort Tamai, Iradj Ashrafi
collection PubMed
description OBJECTIVE(S): Candidiasis infection caused by Candida albicans has been known as a major problem in patients with immune disorders. The objective of this study was to genotype the C. albicans isolates obtained from oral cavity of patients with positive human immunodeficiency virus (HIV(+)) with or/and without oropharyngeal candidiasis (OPC). MATERIALS AND METHODS: A total of 100 C. albicans isolates from Iranian HIV(+)patients were genotyped using specific PCR primers of the 25S rDNA and RPS genes. RESULTS: The frequencies of genotypes A, B and C which were achieved using 25S rDNA , were 66, 24 and 10 percent, respectively. In addition, genotypes D and E were not found in this study. Each C. albicans genotype was further classified into four subtypes (types 2, 3, 2/3 and 3/4) by PCR amplification targeting RPS sequence. CONCLUSION: In general, genotype A3 constituted the majority of understudy clinical isolates obtained from oral cavity of Iranian HIV(+) patients.
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spelling pubmed-43280902015-02-17 Repetitive sequences based on genotyping of Candida albicans isolates obtained from Iranian patients with human immunodeficiency virus Tamai, Iradj Ashrafi Salehi, Taghi Zahraei Sharifzadeh, Aghil Shokri, Hojjatollah Khosravi, Ali Reza Iran J Basic Med Sci Article OBJECTIVE(S): Candidiasis infection caused by Candida albicans has been known as a major problem in patients with immune disorders. The objective of this study was to genotype the C. albicans isolates obtained from oral cavity of patients with positive human immunodeficiency virus (HIV(+)) with or/and without oropharyngeal candidiasis (OPC). MATERIALS AND METHODS: A total of 100 C. albicans isolates from Iranian HIV(+)patients were genotyped using specific PCR primers of the 25S rDNA and RPS genes. RESULTS: The frequencies of genotypes A, B and C which were achieved using 25S rDNA , were 66, 24 and 10 percent, respectively. In addition, genotypes D and E were not found in this study. Each C. albicans genotype was further classified into four subtypes (types 2, 3, 2/3 and 3/4) by PCR amplification targeting RPS sequence. CONCLUSION: In general, genotype A3 constituted the majority of understudy clinical isolates obtained from oral cavity of Iranian HIV(+) patients. Mashhad University of Medical Sciences 2014-11 /pmc/articles/PMC4328090/ /pubmed/25691923 Text en © Iranian Journal of Basic Medical Sciences This is an open-access article distributed under the terms of the Creative Commons Attribution-Noncommercial-Share Alike 3.0 Unported, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Article
Tamai, Iradj Ashrafi
Salehi, Taghi Zahraei
Sharifzadeh, Aghil
Shokri, Hojjatollah
Khosravi, Ali Reza
Repetitive sequences based on genotyping of Candida albicans isolates obtained from Iranian patients with human immunodeficiency virus
title Repetitive sequences based on genotyping of Candida albicans isolates obtained from Iranian patients with human immunodeficiency virus
title_full Repetitive sequences based on genotyping of Candida albicans isolates obtained from Iranian patients with human immunodeficiency virus
title_fullStr Repetitive sequences based on genotyping of Candida albicans isolates obtained from Iranian patients with human immunodeficiency virus
title_full_unstemmed Repetitive sequences based on genotyping of Candida albicans isolates obtained from Iranian patients with human immunodeficiency virus
title_short Repetitive sequences based on genotyping of Candida albicans isolates obtained from Iranian patients with human immunodeficiency virus
title_sort repetitive sequences based on genotyping of candida albicans isolates obtained from iranian patients with human immunodeficiency virus
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4328090/
https://www.ncbi.nlm.nih.gov/pubmed/25691923
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