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Cortical Contractility Triggers a Stochastic Switch to Fast Amoeboid Cell Motility

3D amoeboid cell migration is central to many developmental and disease-related processes such as cancer metastasis. Here, we identify a unique prototypic amoeboid cell migration mode in early zebrafish embryos, termed stable-bleb migration. Stable-bleb cells display an invariant polarized balloon-l...

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Detalles Bibliográficos
Autores principales: Ruprecht, Verena, Wieser, Stefan, Callan-Jones, Andrew, Smutny, Michael, Morita, Hitoshi, Sako, Keisuke, Barone, Vanessa, Ritsch-Marte, Monika, Sixt, Michael, Voituriez, Raphaël, Heisenberg, Carl-Philipp
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Cell Press 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4328143/
https://www.ncbi.nlm.nih.gov/pubmed/25679761
http://dx.doi.org/10.1016/j.cell.2015.01.008
Descripción
Sumario:3D amoeboid cell migration is central to many developmental and disease-related processes such as cancer metastasis. Here, we identify a unique prototypic amoeboid cell migration mode in early zebrafish embryos, termed stable-bleb migration. Stable-bleb cells display an invariant polarized balloon-like shape with exceptional migration speed and persistence. Progenitor cells can be reversibly transformed into stable-bleb cells irrespective of their primary fate and motile characteristics by increasing myosin II activity through biochemical or mechanical stimuli. Using a combination of theory and experiments, we show that, in stable-bleb cells, cortical contractility fluctuations trigger a stochastic switch into amoeboid motility, and a positive feedback between cortical flows and gradients in contractility maintains stable-bleb cell polarization. We further show that rearward cortical flows drive stable-bleb cell migration in various adhesive and non-adhesive environments, unraveling a highly versatile amoeboid migration phenotype.