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The composition of the gut microbiota shapes the colon mucus barrier

Two C57BL/6 mice colonies maintained in two rooms of the same specific pathogen-free (SPF) facility were found to have different gut microbiota and a mucus phenotype that was specific for each colony. The thickness and growth of the colon mucus were similar in the two colonies. However, one colony h...

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Autores principales: Jakobsson, Hedvig E, Rodríguez-Piñeiro, Ana M, Schütte, André, Ermund, Anna, Boysen, Preben, Bemark, Mats, Sommer, Felix, Bäckhed, Fredrik, Hansson, Gunnar C, Johansson, Malin EV
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BlackWell Publishing Ltd 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4328744/
https://www.ncbi.nlm.nih.gov/pubmed/25525071
http://dx.doi.org/10.15252/embr.201439263
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author Jakobsson, Hedvig E
Rodríguez-Piñeiro, Ana M
Schütte, André
Ermund, Anna
Boysen, Preben
Bemark, Mats
Sommer, Felix
Bäckhed, Fredrik
Hansson, Gunnar C
Johansson, Malin EV
author_facet Jakobsson, Hedvig E
Rodríguez-Piñeiro, Ana M
Schütte, André
Ermund, Anna
Boysen, Preben
Bemark, Mats
Sommer, Felix
Bäckhed, Fredrik
Hansson, Gunnar C
Johansson, Malin EV
author_sort Jakobsson, Hedvig E
collection PubMed
description Two C57BL/6 mice colonies maintained in two rooms of the same specific pathogen-free (SPF) facility were found to have different gut microbiota and a mucus phenotype that was specific for each colony. The thickness and growth of the colon mucus were similar in the two colonies. However, one colony had mucus that was impenetrable to bacteria or beads the size of bacteria—which is comparable to what we observed in free-living wild mice—whereas the other colony had an inner mucus layer penetrable to bacteria and beads. The different properties of the mucus depended on the microbiota, as they were transmissible by transfer of caecal microbiota to germ-free mice. Mice with an impenetrable mucus layer had increased amounts of Erysipelotrichi, whereas mice with a penetrable mucus layer had higher levels of Proteobacteria and TM7 bacteria in the distal colon mucus. Thus, our study shows that bacteria and their community structure affect mucus barrier properties in ways that can have implications for health and disease. It also highlights that genetically identical animals housed in the same facility can have rather distinct microbiotas and barrier structures.
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spelling pubmed-43287442015-03-10 The composition of the gut microbiota shapes the colon mucus barrier Jakobsson, Hedvig E Rodríguez-Piñeiro, Ana M Schütte, André Ermund, Anna Boysen, Preben Bemark, Mats Sommer, Felix Bäckhed, Fredrik Hansson, Gunnar C Johansson, Malin EV EMBO Rep Scientific Reports Two C57BL/6 mice colonies maintained in two rooms of the same specific pathogen-free (SPF) facility were found to have different gut microbiota and a mucus phenotype that was specific for each colony. The thickness and growth of the colon mucus were similar in the two colonies. However, one colony had mucus that was impenetrable to bacteria or beads the size of bacteria—which is comparable to what we observed in free-living wild mice—whereas the other colony had an inner mucus layer penetrable to bacteria and beads. The different properties of the mucus depended on the microbiota, as they were transmissible by transfer of caecal microbiota to germ-free mice. Mice with an impenetrable mucus layer had increased amounts of Erysipelotrichi, whereas mice with a penetrable mucus layer had higher levels of Proteobacteria and TM7 bacteria in the distal colon mucus. Thus, our study shows that bacteria and their community structure affect mucus barrier properties in ways that can have implications for health and disease. It also highlights that genetically identical animals housed in the same facility can have rather distinct microbiotas and barrier structures. BlackWell Publishing Ltd 2015-02 2014-12-18 /pmc/articles/PMC4328744/ /pubmed/25525071 http://dx.doi.org/10.15252/embr.201439263 Text en © 2014 The Authors. Published under the terms of the CC BY NC ND 4.0 license http://creativecommons.org/licenses/by-nc-nd/4.0/ This is an open access article under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs 4.0 License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.
spellingShingle Scientific Reports
Jakobsson, Hedvig E
Rodríguez-Piñeiro, Ana M
Schütte, André
Ermund, Anna
Boysen, Preben
Bemark, Mats
Sommer, Felix
Bäckhed, Fredrik
Hansson, Gunnar C
Johansson, Malin EV
The composition of the gut microbiota shapes the colon mucus barrier
title The composition of the gut microbiota shapes the colon mucus barrier
title_full The composition of the gut microbiota shapes the colon mucus barrier
title_fullStr The composition of the gut microbiota shapes the colon mucus barrier
title_full_unstemmed The composition of the gut microbiota shapes the colon mucus barrier
title_short The composition of the gut microbiota shapes the colon mucus barrier
title_sort composition of the gut microbiota shapes the colon mucus barrier
topic Scientific Reports
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4328744/
https://www.ncbi.nlm.nih.gov/pubmed/25525071
http://dx.doi.org/10.15252/embr.201439263
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