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Establishment of new predictive markers for distant recurrence of colorectal cancer using lectin microarray analysis
We evaluated the clinical benefits of novel predictive markers for distant recurrence with colorectal cancer using lectin microarray analysis of cell surface glycan modifications. Glycoproteins were extracted from formalin-fixed, paraffin-embedded tumor specimens and normal epithelium from 53 consec...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BlackWell Publishing Ltd
2015
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4329012/ https://www.ncbi.nlm.nih.gov/pubmed/25355679 http://dx.doi.org/10.1002/cam4.342 |
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author | Nakajima, Kentaro Inomata, Masafumi Iha, Hidekatsu Hiratsuka, Takahiro Etoh, Tsuyoshi Shiraishi, Norio Kashima, Kenji Kitano, Seigo |
author_facet | Nakajima, Kentaro Inomata, Masafumi Iha, Hidekatsu Hiratsuka, Takahiro Etoh, Tsuyoshi Shiraishi, Norio Kashima, Kenji Kitano, Seigo |
author_sort | Nakajima, Kentaro |
collection | PubMed |
description | We evaluated the clinical benefits of novel predictive markers for distant recurrence with colorectal cancer using lectin microarray analysis of cell surface glycan modifications. Glycoproteins were extracted from formalin-fixed, paraffin-embedded tumor specimens and normal epithelium from 53 consecutive curatively resected stage I–III colorectal cancer cases and then subjected to lectin microarray to obtain lectin–glycan interaction (LGI) values. In addition, clinicopathological factors associated with distant recurrence were identified. LGI values that were associated with distant recurrence were validated with an additional 55 curatively resected stage II colorectal cancer cases. LGI values for Agaricus bisporus (ABA) lectin, prominent in cancer tissues, were statistically associated with distant recurrence. ABA lectin staining exhibited strikingly intense signals in the cytoplasm and apical surfaces of cancer cells, while weak staining was observed in the supranuclear regions of normal epithelium. This ABA tumor/normal LGI ratio may be a new predictive biomarker for distant recurrence of curatively resected colorectal cancer. |
format | Online Article Text |
id | pubmed-4329012 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2015 |
publisher | BlackWell Publishing Ltd |
record_format | MEDLINE/PubMed |
spelling | pubmed-43290122015-02-17 Establishment of new predictive markers for distant recurrence of colorectal cancer using lectin microarray analysis Nakajima, Kentaro Inomata, Masafumi Iha, Hidekatsu Hiratsuka, Takahiro Etoh, Tsuyoshi Shiraishi, Norio Kashima, Kenji Kitano, Seigo Cancer Med Cancer Prevention We evaluated the clinical benefits of novel predictive markers for distant recurrence with colorectal cancer using lectin microarray analysis of cell surface glycan modifications. Glycoproteins were extracted from formalin-fixed, paraffin-embedded tumor specimens and normal epithelium from 53 consecutive curatively resected stage I–III colorectal cancer cases and then subjected to lectin microarray to obtain lectin–glycan interaction (LGI) values. In addition, clinicopathological factors associated with distant recurrence were identified. LGI values that were associated with distant recurrence were validated with an additional 55 curatively resected stage II colorectal cancer cases. LGI values for Agaricus bisporus (ABA) lectin, prominent in cancer tissues, were statistically associated with distant recurrence. ABA lectin staining exhibited strikingly intense signals in the cytoplasm and apical surfaces of cancer cells, while weak staining was observed in the supranuclear regions of normal epithelium. This ABA tumor/normal LGI ratio may be a new predictive biomarker for distant recurrence of curatively resected colorectal cancer. BlackWell Publishing Ltd 2015-02 2014-10-30 /pmc/articles/PMC4329012/ /pubmed/25355679 http://dx.doi.org/10.1002/cam4.342 Text en © 2014 The Authors. Cancer Medicine published by John Wiley & Sons Ltd. http://creativecommons.org/licenses/by/3.0/ This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Cancer Prevention Nakajima, Kentaro Inomata, Masafumi Iha, Hidekatsu Hiratsuka, Takahiro Etoh, Tsuyoshi Shiraishi, Norio Kashima, Kenji Kitano, Seigo Establishment of new predictive markers for distant recurrence of colorectal cancer using lectin microarray analysis |
title | Establishment of new predictive markers for distant recurrence of colorectal cancer using lectin microarray analysis |
title_full | Establishment of new predictive markers for distant recurrence of colorectal cancer using lectin microarray analysis |
title_fullStr | Establishment of new predictive markers for distant recurrence of colorectal cancer using lectin microarray analysis |
title_full_unstemmed | Establishment of new predictive markers for distant recurrence of colorectal cancer using lectin microarray analysis |
title_short | Establishment of new predictive markers for distant recurrence of colorectal cancer using lectin microarray analysis |
title_sort | establishment of new predictive markers for distant recurrence of colorectal cancer using lectin microarray analysis |
topic | Cancer Prevention |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4329012/ https://www.ncbi.nlm.nih.gov/pubmed/25355679 http://dx.doi.org/10.1002/cam4.342 |
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