Cargando…

Characterization of one sheep border disease virus in China

BACKGROUND: Border disease virus (BDV) causes border disease (BD) affecting mainly sheep and goats worldwide. BDV in goat herds suffering diarrhea was recently reported in China, however, infection in sheep was undetermined. Here, BDV infections of sheep herds in Jiangsu, China were screened; a BDV...

Descripción completa

Detalles Bibliográficos
Autores principales: Mao, Li, Liu, Xia, Li, Wenliang, Yang, Leilei, Zhang, Wenwen, Jiang, Jieyuan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4329205/
https://www.ncbi.nlm.nih.gov/pubmed/25890073
http://dx.doi.org/10.1186/s12985-014-0217-9
_version_ 1782357401661341696
author Mao, Li
Liu, Xia
Li, Wenliang
Yang, Leilei
Zhang, Wenwen
Jiang, Jieyuan
author_facet Mao, Li
Liu, Xia
Li, Wenliang
Yang, Leilei
Zhang, Wenwen
Jiang, Jieyuan
author_sort Mao, Li
collection PubMed
description BACKGROUND: Border disease virus (BDV) causes border disease (BD) affecting mainly sheep and goats worldwide. BDV in goat herds suffering diarrhea was recently reported in China, however, infection in sheep was undetermined. Here, BDV infections of sheep herds in Jiangsu, China were screened; a BDV strain was isolated and identified from the sheep flocks in China. The genomic characteristics and pathogenesis of this new isolate were studied. RESULTS: In 2012, samples from 160 animals in 5 regions of Jiangsu province of China were screened for the presence of BDV genomic RNA and antibody by RT-PCR and ELISA, respectively. 44.4% of the sera were detected positively, and one slowly grown sheep was analyzed to be pestivirus RNA positive and antibody-negative. The sheep kept virus positive and antibody negative in the next 6 months of whole fattening period, and was defined as persistent infection (PI). The virus was isolated in MDBK cells without cytopathic effect (CPE) and named as JSLS12-01. Near-full-length genome sequenced was 12,227 nucleotides (nt). Phylogenetic analysis based on 5'-UTR and N(pro) fragments showed that the strain belonged to genotype 3, and shared varied homology with the other 3 BDV strains previously isolated from Chinese goats. The genome sequence of JSLS12-01 also had the highest homology with genotype BDV-3 (the strain Gifhorn). Experimental infections of sheep had mild clinical signs as depression and short-period mild fever (5 days). Viremia was detected in 1–7 days post-infection (dpi), and seroconversion began after 14 dpi. CONCLUSIONS: This study reported the genomic and pathogenesis characterizations of one sheep BDV strain, which confirmed the occurrence of BDV infection in Chinese sheep. This sheep derived BDV strain was classified as BDV-3, together with the goat derived strains in China. These results might be helpful for further understanding of BDV infection in China and useful for prevention and control of BDV infections in the future.
format Online
Article
Text
id pubmed-4329205
institution National Center for Biotechnology Information
language English
publishDate 2015
publisher BioMed Central
record_format MEDLINE/PubMed
spelling pubmed-43292052015-02-16 Characterization of one sheep border disease virus in China Mao, Li Liu, Xia Li, Wenliang Yang, Leilei Zhang, Wenwen Jiang, Jieyuan Virol J Research BACKGROUND: Border disease virus (BDV) causes border disease (BD) affecting mainly sheep and goats worldwide. BDV in goat herds suffering diarrhea was recently reported in China, however, infection in sheep was undetermined. Here, BDV infections of sheep herds in Jiangsu, China were screened; a BDV strain was isolated and identified from the sheep flocks in China. The genomic characteristics and pathogenesis of this new isolate were studied. RESULTS: In 2012, samples from 160 animals in 5 regions of Jiangsu province of China were screened for the presence of BDV genomic RNA and antibody by RT-PCR and ELISA, respectively. 44.4% of the sera were detected positively, and one slowly grown sheep was analyzed to be pestivirus RNA positive and antibody-negative. The sheep kept virus positive and antibody negative in the next 6 months of whole fattening period, and was defined as persistent infection (PI). The virus was isolated in MDBK cells without cytopathic effect (CPE) and named as JSLS12-01. Near-full-length genome sequenced was 12,227 nucleotides (nt). Phylogenetic analysis based on 5'-UTR and N(pro) fragments showed that the strain belonged to genotype 3, and shared varied homology with the other 3 BDV strains previously isolated from Chinese goats. The genome sequence of JSLS12-01 also had the highest homology with genotype BDV-3 (the strain Gifhorn). Experimental infections of sheep had mild clinical signs as depression and short-period mild fever (5 days). Viremia was detected in 1–7 days post-infection (dpi), and seroconversion began after 14 dpi. CONCLUSIONS: This study reported the genomic and pathogenesis characterizations of one sheep BDV strain, which confirmed the occurrence of BDV infection in Chinese sheep. This sheep derived BDV strain was classified as BDV-3, together with the goat derived strains in China. These results might be helpful for further understanding of BDV infection in China and useful for prevention and control of BDV infections in the future. BioMed Central 2015-02-08 /pmc/articles/PMC4329205/ /pubmed/25890073 http://dx.doi.org/10.1186/s12985-014-0217-9 Text en © Mao et al.; licensee BioMed Central. 2015 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Research
Mao, Li
Liu, Xia
Li, Wenliang
Yang, Leilei
Zhang, Wenwen
Jiang, Jieyuan
Characterization of one sheep border disease virus in China
title Characterization of one sheep border disease virus in China
title_full Characterization of one sheep border disease virus in China
title_fullStr Characterization of one sheep border disease virus in China
title_full_unstemmed Characterization of one sheep border disease virus in China
title_short Characterization of one sheep border disease virus in China
title_sort characterization of one sheep border disease virus in china
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4329205/
https://www.ncbi.nlm.nih.gov/pubmed/25890073
http://dx.doi.org/10.1186/s12985-014-0217-9
work_keys_str_mv AT maoli characterizationofonesheepborderdiseasevirusinchina
AT liuxia characterizationofonesheepborderdiseasevirusinchina
AT liwenliang characterizationofonesheepborderdiseasevirusinchina
AT yangleilei characterizationofonesheepborderdiseasevirusinchina
AT zhangwenwen characterizationofonesheepborderdiseasevirusinchina
AT jiangjieyuan characterizationofonesheepborderdiseasevirusinchina