Visualization of Endogenous and Exogenous Hydrogen Peroxide Using A Lysosome-Targetable Fluorescent Probe

Reactive oxygen species (ROS) play crucial roles in diverse physiological processes; therefore, the efficient detection of ROS is very crucial. In this study, we report a boronate-based hydrogen peroxide (H(2)O(2)) probe having naphthalimide fluorophore. This probe also contained a morpholine moiety...

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Detalles Bibliográficos
Autores principales: Kim, Dabin, Kim, Gyoungmi, Nam, Sang-Jip, Yin, Jun, Yoon, Juyoung
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2015
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4329546/
https://www.ncbi.nlm.nih.gov/pubmed/25684681
http://dx.doi.org/10.1038/srep08488
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author Kim, Dabin
Kim, Gyoungmi
Nam, Sang-Jip
Yin, Jun
Yoon, Juyoung
author_facet Kim, Dabin
Kim, Gyoungmi
Nam, Sang-Jip
Yin, Jun
Yoon, Juyoung
author_sort Kim, Dabin
collection PubMed
description Reactive oxygen species (ROS) play crucial roles in diverse physiological processes; therefore, the efficient detection of ROS is very crucial. In this study, we report a boronate-based hydrogen peroxide (H(2)O(2)) probe having naphthalimide fluorophore. This probe also contained a morpholine moiety as a directing group for lysosome. The recognition property indicated that the probe exhibited high selectivity towards H(2)O(2) not only in the solution but also in the living cells. Furthermore, it was used to monitor the level of endogenous and exogenous H(2)O(2). These results support that the probe can function as an efficient indicator to detect H(2)O(2).
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spelling pubmed-43295462015-02-23 Visualization of Endogenous and Exogenous Hydrogen Peroxide Using A Lysosome-Targetable Fluorescent Probe Kim, Dabin Kim, Gyoungmi Nam, Sang-Jip Yin, Jun Yoon, Juyoung Sci Rep Article Reactive oxygen species (ROS) play crucial roles in diverse physiological processes; therefore, the efficient detection of ROS is very crucial. In this study, we report a boronate-based hydrogen peroxide (H(2)O(2)) probe having naphthalimide fluorophore. This probe also contained a morpholine moiety as a directing group for lysosome. The recognition property indicated that the probe exhibited high selectivity towards H(2)O(2) not only in the solution but also in the living cells. Furthermore, it was used to monitor the level of endogenous and exogenous H(2)O(2). These results support that the probe can function as an efficient indicator to detect H(2)O(2). Nature Publishing Group 2015-02-16 /pmc/articles/PMC4329546/ /pubmed/25684681 http://dx.doi.org/10.1038/srep08488 Text en Copyright © 2015, Macmillan Publishers Limited. All rights reserved http://creativecommons.org/licenses/by-nc-nd/4.0/ This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivs 4.0 International License. The images or other third party material in this article are included in the article's Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder in order to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by-nc-nd/4.0/
spellingShingle Article
Kim, Dabin
Kim, Gyoungmi
Nam, Sang-Jip
Yin, Jun
Yoon, Juyoung
Visualization of Endogenous and Exogenous Hydrogen Peroxide Using A Lysosome-Targetable Fluorescent Probe
title Visualization of Endogenous and Exogenous Hydrogen Peroxide Using A Lysosome-Targetable Fluorescent Probe
title_full Visualization of Endogenous and Exogenous Hydrogen Peroxide Using A Lysosome-Targetable Fluorescent Probe
title_fullStr Visualization of Endogenous and Exogenous Hydrogen Peroxide Using A Lysosome-Targetable Fluorescent Probe
title_full_unstemmed Visualization of Endogenous and Exogenous Hydrogen Peroxide Using A Lysosome-Targetable Fluorescent Probe
title_short Visualization of Endogenous and Exogenous Hydrogen Peroxide Using A Lysosome-Targetable Fluorescent Probe
title_sort visualization of endogenous and exogenous hydrogen peroxide using a lysosome-targetable fluorescent probe
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4329546/
https://www.ncbi.nlm.nih.gov/pubmed/25684681
http://dx.doi.org/10.1038/srep08488
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