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ZAP70 Expression Within del6q21, del11q13 and del17p13 Cytogenetic Subgroups of Iranian Patients with Chronic Lymphocytic Leukemia

BACKGROUND: B-cell chronic lymphocytic leukemia (B-CLL) is the most common form of leukemia in adults. Some reports showed that expression of ZAP70 gene and chromosomal abnormality are two prognostic factors in management of B-CLL OBJECTIVES: In this study, we determined ZAP70 mRNA expression level...

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Autores principales: Jafari Ghahfarokhi, Hamideh, Ashoori, Saeede, Akbari, Mohamad Taghi, Lotfizadeh, Masoud, Karimi, Ali, Teimori, Hossein
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Kowsar 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4329929/
https://www.ncbi.nlm.nih.gov/pubmed/25763225
http://dx.doi.org/10.5812/ircmj.22528
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author Jafari Ghahfarokhi, Hamideh
Ashoori, Saeede
Akbari, Mohamad Taghi
Lotfizadeh, Masoud
Karimi, Ali
Teimori, Hossein
author_facet Jafari Ghahfarokhi, Hamideh
Ashoori, Saeede
Akbari, Mohamad Taghi
Lotfizadeh, Masoud
Karimi, Ali
Teimori, Hossein
author_sort Jafari Ghahfarokhi, Hamideh
collection PubMed
description BACKGROUND: B-cell chronic lymphocytic leukemia (B-CLL) is the most common form of leukemia in adults. Some reports showed that expression of ZAP70 gene and chromosomal abnormality are two prognostic factors in management of B-CLL OBJECTIVES: In this study, we determined ZAP70 mRNA expression level in the del17p13, del6q21 and del11q13 subgroups of Iranian B-CLL patients to investigate prognostic value of ZAP70 expression. PATIENTS AND METHODS: In this cross-sectional study, fluorescence in situ hybridization analysis was carried out on 66 Iranian B-CLL patients. Zap70 mRNA expression was evaluated by using Real Time RT-PCR. RESULTS: Molecular analysis showed that ZAP70 expression increased 2.46 fold in the del11q13 subgroup, 2.87 fold in the del17p13 and 1.87 fold in the del6q21, compared to the 15 patients in the control group. Comparison of standard deviation and mean of the ZAP70 expression profile within the subgroups showed more variability among the cases of the del11q13 and del17p13 versus tight clustering for the del6q21. Therefore, there is a relation between del6q21 aberrations; which has good prognosis with normal levels of ZAP70 expression. CONCLUSIONS: The results of ANOVA test showed that ZAP70 expression gene was significantly increased in del17p13 and del11q13 subgroups compared to control group. Thus, ZAP70 may play an important role in the prognosis of B-CLL patients.
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spelling pubmed-43299292015-03-11 ZAP70 Expression Within del6q21, del11q13 and del17p13 Cytogenetic Subgroups of Iranian Patients with Chronic Lymphocytic Leukemia Jafari Ghahfarokhi, Hamideh Ashoori, Saeede Akbari, Mohamad Taghi Lotfizadeh, Masoud Karimi, Ali Teimori, Hossein Iran Red Crescent Med J Research Article BACKGROUND: B-cell chronic lymphocytic leukemia (B-CLL) is the most common form of leukemia in adults. Some reports showed that expression of ZAP70 gene and chromosomal abnormality are two prognostic factors in management of B-CLL OBJECTIVES: In this study, we determined ZAP70 mRNA expression level in the del17p13, del6q21 and del11q13 subgroups of Iranian B-CLL patients to investigate prognostic value of ZAP70 expression. PATIENTS AND METHODS: In this cross-sectional study, fluorescence in situ hybridization analysis was carried out on 66 Iranian B-CLL patients. Zap70 mRNA expression was evaluated by using Real Time RT-PCR. RESULTS: Molecular analysis showed that ZAP70 expression increased 2.46 fold in the del11q13 subgroup, 2.87 fold in the del17p13 and 1.87 fold in the del6q21, compared to the 15 patients in the control group. Comparison of standard deviation and mean of the ZAP70 expression profile within the subgroups showed more variability among the cases of the del11q13 and del17p13 versus tight clustering for the del6q21. Therefore, there is a relation between del6q21 aberrations; which has good prognosis with normal levels of ZAP70 expression. CONCLUSIONS: The results of ANOVA test showed that ZAP70 expression gene was significantly increased in del17p13 and del11q13 subgroups compared to control group. Thus, ZAP70 may play an important role in the prognosis of B-CLL patients. Kowsar 2014-11-16 /pmc/articles/PMC4329929/ /pubmed/25763225 http://dx.doi.org/10.5812/ircmj.22528 Text en Copyright © 2014, Iranian Red Crescent Medical Journal. http://creativecommons.org/licenses/by-nc/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International License (http://creativecommons.org/licenses/by-nc/4.0/) which permits copy and redistribute the material just in noncommercial usages, provided the original work is properly cited.
spellingShingle Research Article
Jafari Ghahfarokhi, Hamideh
Ashoori, Saeede
Akbari, Mohamad Taghi
Lotfizadeh, Masoud
Karimi, Ali
Teimori, Hossein
ZAP70 Expression Within del6q21, del11q13 and del17p13 Cytogenetic Subgroups of Iranian Patients with Chronic Lymphocytic Leukemia
title ZAP70 Expression Within del6q21, del11q13 and del17p13 Cytogenetic Subgroups of Iranian Patients with Chronic Lymphocytic Leukemia
title_full ZAP70 Expression Within del6q21, del11q13 and del17p13 Cytogenetic Subgroups of Iranian Patients with Chronic Lymphocytic Leukemia
title_fullStr ZAP70 Expression Within del6q21, del11q13 and del17p13 Cytogenetic Subgroups of Iranian Patients with Chronic Lymphocytic Leukemia
title_full_unstemmed ZAP70 Expression Within del6q21, del11q13 and del17p13 Cytogenetic Subgroups of Iranian Patients with Chronic Lymphocytic Leukemia
title_short ZAP70 Expression Within del6q21, del11q13 and del17p13 Cytogenetic Subgroups of Iranian Patients with Chronic Lymphocytic Leukemia
title_sort zap70 expression within del6q21, del11q13 and del17p13 cytogenetic subgroups of iranian patients with chronic lymphocytic leukemia
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4329929/
https://www.ncbi.nlm.nih.gov/pubmed/25763225
http://dx.doi.org/10.5812/ircmj.22528
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