SILAC-iPAC: A quantitative method for distinguishing genuine from non-specific components of protein complexes by parallel affinity capture

Pull-down assays can identify members of protein complexes but suffer from co-isolation of contaminants. The problem is particularly acute when the specifically interacting partners are of low-abundance and/or bind transiently with low affinity. To differentiate true interacting partners from contam...

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Detalles Bibliográficos
Autores principales: Rees, Johanna S., Lilley, Kathryn S., Jackson, Antony P.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2015
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4329988/
https://www.ncbi.nlm.nih.gov/pubmed/25534881
http://dx.doi.org/10.1016/j.jprot.2014.12.006

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4329988/
https://www.ncbi.nlm.nih.gov/pubmed/25534881
http://dx.doi.org/10.1016/j.jprot.2014.12.006

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