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Detection of Encephalitozoon spp. from Human Diarrheal Stool and Farm Soil Samples in Korea

Microsporidia are eukaryotic organisms that cause zoonosis and are major opportunistic pathogens in HIV-positive patients. However, there is increasing evidence that these organisms can also cause gastrointestinal and ocular infections in immunocompetent individuals. In Korea, there have been no rep...

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Autores principales: Kim, Kyungjin, Yoon, Sejoung, Cheun, Hyeng-Il, Kim, Jae-Hwan, Sim, Seobo, Yu, Jae-Ran
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Korean Academy of Medical Sciences 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4330474/
https://www.ncbi.nlm.nih.gov/pubmed/25729242
http://dx.doi.org/10.3346/jkms.2015.30.3.227
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author Kim, Kyungjin
Yoon, Sejoung
Cheun, Hyeng-Il
Kim, Jae-Hwan
Sim, Seobo
Yu, Jae-Ran
author_facet Kim, Kyungjin
Yoon, Sejoung
Cheun, Hyeng-Il
Kim, Jae-Hwan
Sim, Seobo
Yu, Jae-Ran
author_sort Kim, Kyungjin
collection PubMed
description Microsporidia are eukaryotic organisms that cause zoonosis and are major opportunistic pathogens in HIV-positive patients. However, there is increasing evidence that these organisms can also cause gastrointestinal and ocular infections in immunocompetent individuals. In Korea, there have been no reports on human infections with microsporidia to date. In the present study, we used real-time PCR and nucleotide sequencing to detect Encephalitozoon intestinalis infection in seven of 139 human diarrheal stool specimens (5%) and Encephalitozoon hellem in three of 34 farm soil samples (8.8%). Genotype analysis of the E. hellem isolates based on the internal transcribed spacer 1 and polar tube protein genes showed that all isolates were genotype 1B. To our knowledge, this is the first report on human E. intestinalis infection in Korea and the first report revealing farm soil samples as a source of E. hellem infection. Because microsporidia are an important public health issue, further large-scale epidemiological studies are warranted. GRAPHICAL ABSTRACT: [Image: see text]
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spelling pubmed-43304742015-03-01 Detection of Encephalitozoon spp. from Human Diarrheal Stool and Farm Soil Samples in Korea Kim, Kyungjin Yoon, Sejoung Cheun, Hyeng-Il Kim, Jae-Hwan Sim, Seobo Yu, Jae-Ran J Korean Med Sci Original Article Microsporidia are eukaryotic organisms that cause zoonosis and are major opportunistic pathogens in HIV-positive patients. However, there is increasing evidence that these organisms can also cause gastrointestinal and ocular infections in immunocompetent individuals. In Korea, there have been no reports on human infections with microsporidia to date. In the present study, we used real-time PCR and nucleotide sequencing to detect Encephalitozoon intestinalis infection in seven of 139 human diarrheal stool specimens (5%) and Encephalitozoon hellem in three of 34 farm soil samples (8.8%). Genotype analysis of the E. hellem isolates based on the internal transcribed spacer 1 and polar tube protein genes showed that all isolates were genotype 1B. To our knowledge, this is the first report on human E. intestinalis infection in Korea and the first report revealing farm soil samples as a source of E. hellem infection. Because microsporidia are an important public health issue, further large-scale epidemiological studies are warranted. GRAPHICAL ABSTRACT: [Image: see text] The Korean Academy of Medical Sciences 2015-03 2015-02-16 /pmc/articles/PMC4330474/ /pubmed/25729242 http://dx.doi.org/10.3346/jkms.2015.30.3.227 Text en © 2015 The Korean Academy of Medical Sciences. http://creativecommons.org/licenses/by-nc/4.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Article
Kim, Kyungjin
Yoon, Sejoung
Cheun, Hyeng-Il
Kim, Jae-Hwan
Sim, Seobo
Yu, Jae-Ran
Detection of Encephalitozoon spp. from Human Diarrheal Stool and Farm Soil Samples in Korea
title Detection of Encephalitozoon spp. from Human Diarrheal Stool and Farm Soil Samples in Korea
title_full Detection of Encephalitozoon spp. from Human Diarrheal Stool and Farm Soil Samples in Korea
title_fullStr Detection of Encephalitozoon spp. from Human Diarrheal Stool and Farm Soil Samples in Korea
title_full_unstemmed Detection of Encephalitozoon spp. from Human Diarrheal Stool and Farm Soil Samples in Korea
title_short Detection of Encephalitozoon spp. from Human Diarrheal Stool and Farm Soil Samples in Korea
title_sort detection of encephalitozoon spp. from human diarrheal stool and farm soil samples in korea
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4330474/
https://www.ncbi.nlm.nih.gov/pubmed/25729242
http://dx.doi.org/10.3346/jkms.2015.30.3.227
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