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Isolation and Molecular Identification of Mycoplasma Hominis in Infertile Female and Male Reproductive System

BACKGROUND: Infection of urogenital system with Mycoplasma potentially affect reproductive system and increases infants mortalities. Therefore, detection of these organisms is an important issue that should be considered and appropriate diagnostic methods should be used to identify these microorgani...

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Autores principales: Jamalizadeh Bahaabadi, Samaneh, Mohseni Moghadam, Naeime, Kheirkhah, Babak, Farsinejad, Alireza, Habibzadeh, Victoria
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Kowsar 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4330675/
https://www.ncbi.nlm.nih.gov/pubmed/25738116
http://dx.doi.org/10.5812/numonthly.22390
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author Jamalizadeh Bahaabadi, Samaneh
Mohseni Moghadam, Naeime
Kheirkhah, Babak
Farsinejad, Alireza
Habibzadeh, Victoria
author_facet Jamalizadeh Bahaabadi, Samaneh
Mohseni Moghadam, Naeime
Kheirkhah, Babak
Farsinejad, Alireza
Habibzadeh, Victoria
author_sort Jamalizadeh Bahaabadi, Samaneh
collection PubMed
description BACKGROUND: Infection of urogenital system with Mycoplasma potentially affect reproductive system and increases infants mortalities. Therefore, detection of these organisms is an important issue that should be considered and appropriate diagnostic methods should be used to identify these microorganisms. In the female reproductive system, infection can affect different parts of the cervix, endometrium, and fallopian tube. The extent of this infection in different diseases and its pathogenesis might be related to anatomic site of involvement. Some infections can lead to infertility in both males and females. Genital infection with Mycoplasmas have devastating effects on reproductive organs and cause fertility disorders and mortality in infants. In recent years, many studies have been conducted to isolate these pathogens; however, the isolates have not been identified so far. OBJECTIVES: The aim of this study was to determine the molecular identity of Mycoplasma hominis isolated from infertile female and male reproductive system in the Infertility Center of Kerman. MATERIALS AND METHODS: This descriptive study was performed purposefully on 100 infertile females and 100 infertile males who were referred to the Infertility Center of Kerman during a six-month period. The collected samples of semen and vaginal swabs were examined for the presence of M. hominis by PCR. The samples with positive results in PCR were selected for molecular identification. Alignment of samples sequence was performed using MEGA 5 software through Neighbor-joining method. RESULTS: Among 100 samples from infertile males, the presence of genus Mycoplasma was confirmed in 45 cases of which 15 cases were infected with M. hominis. Among 100 samples from infertile female, the presence of genus Mycoplasma was confirmed in 43 cases of which 18 case were infected with M. hominis. The positive samples were sequenced and the phylogenetic tree was plotted. CONCLUSIONS: The results showed that 37.5% of infertile males and females were infected with M. hominis. Analysis of the nucleotide sequences of the study isolates indicates a particular variety among these isolates. In comparing the isolates in the study, a very little genotypic similarity was found among some of them.
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spelling pubmed-43306752015-03-03 Isolation and Molecular Identification of Mycoplasma Hominis in Infertile Female and Male Reproductive System Jamalizadeh Bahaabadi, Samaneh Mohseni Moghadam, Naeime Kheirkhah, Babak Farsinejad, Alireza Habibzadeh, Victoria Nephrourol Mon Research Article BACKGROUND: Infection of urogenital system with Mycoplasma potentially affect reproductive system and increases infants mortalities. Therefore, detection of these organisms is an important issue that should be considered and appropriate diagnostic methods should be used to identify these microorganisms. In the female reproductive system, infection can affect different parts of the cervix, endometrium, and fallopian tube. The extent of this infection in different diseases and its pathogenesis might be related to anatomic site of involvement. Some infections can lead to infertility in both males and females. Genital infection with Mycoplasmas have devastating effects on reproductive organs and cause fertility disorders and mortality in infants. In recent years, many studies have been conducted to isolate these pathogens; however, the isolates have not been identified so far. OBJECTIVES: The aim of this study was to determine the molecular identity of Mycoplasma hominis isolated from infertile female and male reproductive system in the Infertility Center of Kerman. MATERIALS AND METHODS: This descriptive study was performed purposefully on 100 infertile females and 100 infertile males who were referred to the Infertility Center of Kerman during a six-month period. The collected samples of semen and vaginal swabs were examined for the presence of M. hominis by PCR. The samples with positive results in PCR were selected for molecular identification. Alignment of samples sequence was performed using MEGA 5 software through Neighbor-joining method. RESULTS: Among 100 samples from infertile males, the presence of genus Mycoplasma was confirmed in 45 cases of which 15 cases were infected with M. hominis. Among 100 samples from infertile female, the presence of genus Mycoplasma was confirmed in 43 cases of which 18 case were infected with M. hominis. The positive samples were sequenced and the phylogenetic tree was plotted. CONCLUSIONS: The results showed that 37.5% of infertile males and females were infected with M. hominis. Analysis of the nucleotide sequences of the study isolates indicates a particular variety among these isolates. In comparing the isolates in the study, a very little genotypic similarity was found among some of them. Kowsar 2014-11-11 /pmc/articles/PMC4330675/ /pubmed/25738116 http://dx.doi.org/10.5812/numonthly.22390 Text en Copyright © 2014, Nephrology and Urology Research Center. http://creativecommons.org/licenses/by-nc/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International License (http://creativecommons.org/licenses/by-nc/4.0/) which permits copy and redistribute the material just in noncommercial usages, provided the original work is properly cited.
spellingShingle Research Article
Jamalizadeh Bahaabadi, Samaneh
Mohseni Moghadam, Naeime
Kheirkhah, Babak
Farsinejad, Alireza
Habibzadeh, Victoria
Isolation and Molecular Identification of Mycoplasma Hominis in Infertile Female and Male Reproductive System
title Isolation and Molecular Identification of Mycoplasma Hominis in Infertile Female and Male Reproductive System
title_full Isolation and Molecular Identification of Mycoplasma Hominis in Infertile Female and Male Reproductive System
title_fullStr Isolation and Molecular Identification of Mycoplasma Hominis in Infertile Female and Male Reproductive System
title_full_unstemmed Isolation and Molecular Identification of Mycoplasma Hominis in Infertile Female and Male Reproductive System
title_short Isolation and Molecular Identification of Mycoplasma Hominis in Infertile Female and Male Reproductive System
title_sort isolation and molecular identification of mycoplasma hominis in infertile female and male reproductive system
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4330675/
https://www.ncbi.nlm.nih.gov/pubmed/25738116
http://dx.doi.org/10.5812/numonthly.22390
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