The Biophysical Properties of Basal Lamina Gels Depend on the Biochemical Composition of the Gel

The migration of cells within a three-dimensional extracellular matrix (ECM) depends sensitively on the biochemical and biophysical properties of the matrix. An example for a biological ECM is given by reconstituted basal lamina gels purified from the Engelbreth-Holm-Swarm sarcoma of mice. Here, we...

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Autores principales: Arends, Fabienna, Nowald, Constantin, Pflieger, Kerstin, Boettcher, Kathrin, Zahler, Stefan, Lieleg, Oliver
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2015
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4331274/
https://www.ncbi.nlm.nih.gov/pubmed/25689062
http://dx.doi.org/10.1371/journal.pone.0118090
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author Arends, Fabienna
Nowald, Constantin
Pflieger, Kerstin
Boettcher, Kathrin
Zahler, Stefan
Lieleg, Oliver
author_facet Arends, Fabienna
Nowald, Constantin
Pflieger, Kerstin
Boettcher, Kathrin
Zahler, Stefan
Lieleg, Oliver
author_sort Arends, Fabienna
collection PubMed
description The migration of cells within a three-dimensional extracellular matrix (ECM) depends sensitively on the biochemical and biophysical properties of the matrix. An example for a biological ECM is given by reconstituted basal lamina gels purified from the Engelbreth-Holm-Swarm sarcoma of mice. Here, we compare four different commercial variants of this ECM, which have all been purified according to the same protocol. Nevertheless, in those gels, we detect strong differences in the migration behavior of leukocyte cells as well as in the Brownian motion of nanoparticles. We show that these differences correlate with the mechanical properties and the microarchitecture of the gels which in turn arise from small variations in their biochemical composition.
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spelling pubmed-43312742015-02-24 The Biophysical Properties of Basal Lamina Gels Depend on the Biochemical Composition of the Gel Arends, Fabienna Nowald, Constantin Pflieger, Kerstin Boettcher, Kathrin Zahler, Stefan Lieleg, Oliver PLoS One Research Article The migration of cells within a three-dimensional extracellular matrix (ECM) depends sensitively on the biochemical and biophysical properties of the matrix. An example for a biological ECM is given by reconstituted basal lamina gels purified from the Engelbreth-Holm-Swarm sarcoma of mice. Here, we compare four different commercial variants of this ECM, which have all been purified according to the same protocol. Nevertheless, in those gels, we detect strong differences in the migration behavior of leukocyte cells as well as in the Brownian motion of nanoparticles. We show that these differences correlate with the mechanical properties and the microarchitecture of the gels which in turn arise from small variations in their biochemical composition. Public Library of Science 2015-02-17 /pmc/articles/PMC4331274/ /pubmed/25689062 http://dx.doi.org/10.1371/journal.pone.0118090 Text en © 2015 Arends et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Arends, Fabienna
Nowald, Constantin
Pflieger, Kerstin
Boettcher, Kathrin
Zahler, Stefan
Lieleg, Oliver
The Biophysical Properties of Basal Lamina Gels Depend on the Biochemical Composition of the Gel
title The Biophysical Properties of Basal Lamina Gels Depend on the Biochemical Composition of the Gel
title_full The Biophysical Properties of Basal Lamina Gels Depend on the Biochemical Composition of the Gel
title_fullStr The Biophysical Properties of Basal Lamina Gels Depend on the Biochemical Composition of the Gel
title_full_unstemmed The Biophysical Properties of Basal Lamina Gels Depend on the Biochemical Composition of the Gel
title_short The Biophysical Properties of Basal Lamina Gels Depend on the Biochemical Composition of the Gel
title_sort biophysical properties of basal lamina gels depend on the biochemical composition of the gel
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4331274/
https://www.ncbi.nlm.nih.gov/pubmed/25689062
http://dx.doi.org/10.1371/journal.pone.0118090
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