Characterization of LpGPAT Gene in Lilium pensylvanicum and Response to Cold Stress

LpGPAT was obtained from L. pensylvanicum using RT-PCR and rapid amplification of cDNA ends. The cloned full-length cDNA was 1544 bp; it encoded 410 amino acids and had a molecular size of 46 KDa. The nucleic acid sequence analysis showed that it shared high homology with other known GPATs. SMAT res...

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Autores principales: Sun, Shao-kun, Yang, Ni-na, Chen, Li-jing, Irfan, Muhammad, Zhao, Xing-hua, Li, Tian-lai
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi Publishing Corporation 2015
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4331407/
https://www.ncbi.nlm.nih.gov/pubmed/25710023
http://dx.doi.org/10.1155/2015/792819
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author Sun, Shao-kun
Yang, Ni-na
Chen, Li-jing
Irfan, Muhammad
Zhao, Xing-hua
Li, Tian-lai
author_facet Sun, Shao-kun
Yang, Ni-na
Chen, Li-jing
Irfan, Muhammad
Zhao, Xing-hua
Li, Tian-lai
author_sort Sun, Shao-kun
collection PubMed
description LpGPAT was obtained from L. pensylvanicum using RT-PCR and rapid amplification of cDNA ends. The cloned full-length cDNA was 1544 bp; it encoded 410 amino acids and had a molecular size of 46 KDa. The nucleic acid sequence analysis showed that it shared high homology with other known GPATs. SMAT result suggests that there is a PlsC that exists in 176-322 amino acid sequence of LpGAPT; it means LpGPAT protein is a member of the family of acyltransferase and has acyltransferase enzymatic activity. Result of real-time quantitative PCR and semiquantitative PCR support LpGPAT gene is definitely induced by low temperature stress.
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spelling pubmed-43314072015-02-23 Characterization of LpGPAT Gene in Lilium pensylvanicum and Response to Cold Stress Sun, Shao-kun Yang, Ni-na Chen, Li-jing Irfan, Muhammad Zhao, Xing-hua Li, Tian-lai Biomed Res Int Research Article LpGPAT was obtained from L. pensylvanicum using RT-PCR and rapid amplification of cDNA ends. The cloned full-length cDNA was 1544 bp; it encoded 410 amino acids and had a molecular size of 46 KDa. The nucleic acid sequence analysis showed that it shared high homology with other known GPATs. SMAT result suggests that there is a PlsC that exists in 176-322 amino acid sequence of LpGAPT; it means LpGPAT protein is a member of the family of acyltransferase and has acyltransferase enzymatic activity. Result of real-time quantitative PCR and semiquantitative PCR support LpGPAT gene is definitely induced by low temperature stress. Hindawi Publishing Corporation 2015 2015-02-01 /pmc/articles/PMC4331407/ /pubmed/25710023 http://dx.doi.org/10.1155/2015/792819 Text en Copyright © 2015 Shao-kun Sun et al. https://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Sun, Shao-kun
Yang, Ni-na
Chen, Li-jing
Irfan, Muhammad
Zhao, Xing-hua
Li, Tian-lai
Characterization of LpGPAT Gene in Lilium pensylvanicum and Response to Cold Stress
title Characterization of LpGPAT Gene in Lilium pensylvanicum and Response to Cold Stress
title_full Characterization of LpGPAT Gene in Lilium pensylvanicum and Response to Cold Stress
title_fullStr Characterization of LpGPAT Gene in Lilium pensylvanicum and Response to Cold Stress
title_full_unstemmed Characterization of LpGPAT Gene in Lilium pensylvanicum and Response to Cold Stress
title_short Characterization of LpGPAT Gene in Lilium pensylvanicum and Response to Cold Stress
title_sort characterization of lpgpat gene in lilium pensylvanicum and response to cold stress
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4331407/
https://www.ncbi.nlm.nih.gov/pubmed/25710023
http://dx.doi.org/10.1155/2015/792819
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