A Simple Method for Encapsulating Single Cells in Alginate Microspheres Allows for Direct PCR and Whole Genome Amplification

Microdroplets are an effective platform for segregating individual cells and amplifying DNA. However, a key challenge is to recover the contents of individual droplets for downstream analysis. This paper offers a method for embedding cells in alginate microspheres and performing multiple serial oper...

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Detalles Bibliográficos
Autores principales: Bigdeli, Saharnaz, Dettloff, Roger O., Frank, Curtis W., Davis, Ronald W., Crosby, Laurel D.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2015
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4331554/
https://www.ncbi.nlm.nih.gov/pubmed/25689864
http://dx.doi.org/10.1371/journal.pone.0117738

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4331554/
https://www.ncbi.nlm.nih.gov/pubmed/25689864
http://dx.doi.org/10.1371/journal.pone.0117738

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