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Associations among Sebox and Other MEGs and Its Effects on Early Embryogenesis

In a previous report, we identified Sebox as a new candidate maternal effect gene that is essential for embryonic development and primarily impacts the two-cell (2C) stage. The present study was conducted to determine the mechanism of action for Sebox in this capacity, as shown by changes in the exp...

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Autores principales: Park, Min-Woo, Kim, Kyeoung-Hwa, Kim, Eun-Young, Lee, Su-Yeon, Ko, Jung-Jae, Lee, Kyung-Ah
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4331730/
https://www.ncbi.nlm.nih.gov/pubmed/25679966
http://dx.doi.org/10.1371/journal.pone.0115050
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author Park, Min-Woo
Kim, Kyeoung-Hwa
Kim, Eun-Young
Lee, Su-Yeon
Ko, Jung-Jae
Lee, Kyung-Ah
author_facet Park, Min-Woo
Kim, Kyeoung-Hwa
Kim, Eun-Young
Lee, Su-Yeon
Ko, Jung-Jae
Lee, Kyung-Ah
author_sort Park, Min-Woo
collection PubMed
description In a previous report, we identified Sebox as a new candidate maternal effect gene that is essential for embryonic development and primarily impacts the two-cell (2C) stage. The present study was conducted to determine the mechanism of action for Sebox in this capacity, as shown by changes in the expression levels of other known MEG mRNAs after Sebox RNA interference (RNAi) in oocytes. Sebox-knockdown metaphase II (Mll) oocytes displayed normal morphology, but among the 23 MEGs monitored, 8 genes were upregulated, and 15 genes were unchanged. We hypothesized that the perturbed gene expression of these MEGs may cause the arrest of embryo development at the 2C stage and examined the expression of several marker genes for the degradation of maternal factors and zygotic genome activation. We found that some maternal mRNAs, c-mos, Gbx2, and Gdf9, were not fully degraded in Sebox-knockdown 2C embryos, and that several zygotic genome activation markers, Mt1a, Rpl23, Ube2a and Wee1, were not fully expressed in conjunction with diminished embryonic transcriptional activity. In addition, Sebox may be involved in the formation of the subcortical maternal complex through its regulation of the upstream regulator, Figla. Therefore, we concluded that Sebox is important in preparing oocytes for embryonic development by orchestrating the expression of other important MEGs.
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spelling pubmed-43317302015-02-24 Associations among Sebox and Other MEGs and Its Effects on Early Embryogenesis Park, Min-Woo Kim, Kyeoung-Hwa Kim, Eun-Young Lee, Su-Yeon Ko, Jung-Jae Lee, Kyung-Ah PLoS One Research Article In a previous report, we identified Sebox as a new candidate maternal effect gene that is essential for embryonic development and primarily impacts the two-cell (2C) stage. The present study was conducted to determine the mechanism of action for Sebox in this capacity, as shown by changes in the expression levels of other known MEG mRNAs after Sebox RNA interference (RNAi) in oocytes. Sebox-knockdown metaphase II (Mll) oocytes displayed normal morphology, but among the 23 MEGs monitored, 8 genes were upregulated, and 15 genes were unchanged. We hypothesized that the perturbed gene expression of these MEGs may cause the arrest of embryo development at the 2C stage and examined the expression of several marker genes for the degradation of maternal factors and zygotic genome activation. We found that some maternal mRNAs, c-mos, Gbx2, and Gdf9, were not fully degraded in Sebox-knockdown 2C embryos, and that several zygotic genome activation markers, Mt1a, Rpl23, Ube2a and Wee1, were not fully expressed in conjunction with diminished embryonic transcriptional activity. In addition, Sebox may be involved in the formation of the subcortical maternal complex through its regulation of the upstream regulator, Figla. Therefore, we concluded that Sebox is important in preparing oocytes for embryonic development by orchestrating the expression of other important MEGs. Public Library of Science 2015-02-13 /pmc/articles/PMC4331730/ /pubmed/25679966 http://dx.doi.org/10.1371/journal.pone.0115050 Text en © 2015 Park et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Park, Min-Woo
Kim, Kyeoung-Hwa
Kim, Eun-Young
Lee, Su-Yeon
Ko, Jung-Jae
Lee, Kyung-Ah
Associations among Sebox and Other MEGs and Its Effects on Early Embryogenesis
title Associations among Sebox and Other MEGs and Its Effects on Early Embryogenesis
title_full Associations among Sebox and Other MEGs and Its Effects on Early Embryogenesis
title_fullStr Associations among Sebox and Other MEGs and Its Effects on Early Embryogenesis
title_full_unstemmed Associations among Sebox and Other MEGs and Its Effects on Early Embryogenesis
title_short Associations among Sebox and Other MEGs and Its Effects on Early Embryogenesis
title_sort associations among sebox and other megs and its effects on early embryogenesis
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4331730/
https://www.ncbi.nlm.nih.gov/pubmed/25679966
http://dx.doi.org/10.1371/journal.pone.0115050
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