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Apoptosis in capillary endothelial cells in ageing skeletal muscle
The age-related loss of skeletal muscle mass and function (sarcopenia) is a consistent hallmark of ageing. Apoptosis plays an important role in muscle atrophy, and the intent of this study was to specify whether apoptosis is restricted to myofibre nuclei (myonuclei) or occurs in satellite cells or s...
Autores principales: | , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BlackWell Publishing Ltd
2014
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4331780/ https://www.ncbi.nlm.nih.gov/pubmed/24245531 http://dx.doi.org/10.1111/acel.12169 |
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author | Wang, Huijuan Listrat, Anne Meunier, Bruno Gueugneau, Marine Coudy-Gandilhon, Cécile Combaret, Lydie Taillandier, Daniel Polge, Cécile Attaix, Didier Lethias, Claire Lee, Kijoon Goh, Kheng Lim Béchet, Daniel |
author_facet | Wang, Huijuan Listrat, Anne Meunier, Bruno Gueugneau, Marine Coudy-Gandilhon, Cécile Combaret, Lydie Taillandier, Daniel Polge, Cécile Attaix, Didier Lethias, Claire Lee, Kijoon Goh, Kheng Lim Béchet, Daniel |
author_sort | Wang, Huijuan |
collection | PubMed |
description | The age-related loss of skeletal muscle mass and function (sarcopenia) is a consistent hallmark of ageing. Apoptosis plays an important role in muscle atrophy, and the intent of this study was to specify whether apoptosis is restricted to myofibre nuclei (myonuclei) or occurs in satellite cells or stromal cells of extracellular matrix (ECM). Sarcopenia in mouse gastrocnemius muscle was characterized by myofibre atrophy, oxidative type grouping, delocalization of myonuclei and ECM fibrosis. Terminal deoxynucleotidyl transferase-mediated dUTP nick end-labelling (TUNEL) indicated a sharp rise in apoptosis during ageing. TUNEL coupled with immunostaining for dystrophin, paired box protein-7 (Pax7) or laminin-2α, respectively, was used to identify apoptosis in myonuclei, satellite cells and stromal cells. In adult muscle, apoptosis was not detected in myofibres, but was restricted to stromal cells. Moreover, the age-related rise in apoptotic nuclei was essentially due to stromal cells. Myofibre-associated apoptosis nevertheless occurred in old muscle, but represented < 20% of the total muscle apoptosis. Specifically, apoptosis in old muscle affected a small proportion (0.8%) of the myonuclei, but a large part (46%) of the Pax7(+) satellite cells. TUNEL coupled with CD31 immunostaining further attributed stromal apoptosis to capillary endothelial cells. Age-dependent rise in apoptotic capillary endothelial cells was concomitant with altered levels of key angiogenic regulators, perlecan and a perlecan domain V (endorepellin) proteolytic product. Collectively, our results indicate that sarcopenia is associated with apoptosis of satellite cells and impairment of capillary functions, which is likely to contribute to the decline in muscle mass and functionality during ageing. |
format | Online Article Text |
id | pubmed-4331780 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2014 |
publisher | BlackWell Publishing Ltd |
record_format | MEDLINE/PubMed |
spelling | pubmed-43317802015-02-19 Apoptosis in capillary endothelial cells in ageing skeletal muscle Wang, Huijuan Listrat, Anne Meunier, Bruno Gueugneau, Marine Coudy-Gandilhon, Cécile Combaret, Lydie Taillandier, Daniel Polge, Cécile Attaix, Didier Lethias, Claire Lee, Kijoon Goh, Kheng Lim Béchet, Daniel Aging Cell Original Articles The age-related loss of skeletal muscle mass and function (sarcopenia) is a consistent hallmark of ageing. Apoptosis plays an important role in muscle atrophy, and the intent of this study was to specify whether apoptosis is restricted to myofibre nuclei (myonuclei) or occurs in satellite cells or stromal cells of extracellular matrix (ECM). Sarcopenia in mouse gastrocnemius muscle was characterized by myofibre atrophy, oxidative type grouping, delocalization of myonuclei and ECM fibrosis. Terminal deoxynucleotidyl transferase-mediated dUTP nick end-labelling (TUNEL) indicated a sharp rise in apoptosis during ageing. TUNEL coupled with immunostaining for dystrophin, paired box protein-7 (Pax7) or laminin-2α, respectively, was used to identify apoptosis in myonuclei, satellite cells and stromal cells. In adult muscle, apoptosis was not detected in myofibres, but was restricted to stromal cells. Moreover, the age-related rise in apoptotic nuclei was essentially due to stromal cells. Myofibre-associated apoptosis nevertheless occurred in old muscle, but represented < 20% of the total muscle apoptosis. Specifically, apoptosis in old muscle affected a small proportion (0.8%) of the myonuclei, but a large part (46%) of the Pax7(+) satellite cells. TUNEL coupled with CD31 immunostaining further attributed stromal apoptosis to capillary endothelial cells. Age-dependent rise in apoptotic capillary endothelial cells was concomitant with altered levels of key angiogenic regulators, perlecan and a perlecan domain V (endorepellin) proteolytic product. Collectively, our results indicate that sarcopenia is associated with apoptosis of satellite cells and impairment of capillary functions, which is likely to contribute to the decline in muscle mass and functionality during ageing. BlackWell Publishing Ltd 2014-04 2013-11-19 /pmc/articles/PMC4331780/ /pubmed/24245531 http://dx.doi.org/10.1111/acel.12169 Text en © 2013 The Authors. Aging Cell published by the Anatomical Society and John Wiley & Sons Ltd. http://creativecommons.org/licenses/by/3.0/ This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Original Articles Wang, Huijuan Listrat, Anne Meunier, Bruno Gueugneau, Marine Coudy-Gandilhon, Cécile Combaret, Lydie Taillandier, Daniel Polge, Cécile Attaix, Didier Lethias, Claire Lee, Kijoon Goh, Kheng Lim Béchet, Daniel Apoptosis in capillary endothelial cells in ageing skeletal muscle |
title | Apoptosis in capillary endothelial cells in ageing skeletal muscle |
title_full | Apoptosis in capillary endothelial cells in ageing skeletal muscle |
title_fullStr | Apoptosis in capillary endothelial cells in ageing skeletal muscle |
title_full_unstemmed | Apoptosis in capillary endothelial cells in ageing skeletal muscle |
title_short | Apoptosis in capillary endothelial cells in ageing skeletal muscle |
title_sort | apoptosis in capillary endothelial cells in ageing skeletal muscle |
topic | Original Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4331780/ https://www.ncbi.nlm.nih.gov/pubmed/24245531 http://dx.doi.org/10.1111/acel.12169 |
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