Cargando…
Single Intramuscular-dose Toxicity of Anti-inflammatory Pharmacopuncture in Rats
OBJECTIVES: This study was performed to analyze the toxicity of the test substance, anti-inflammatory pharmacopuncture (AIP), when used as a single intramuscular-dose in 6-week-old, male and female Sprague-Dawley rats and to find the lethal dose. METHODS: The experiment was conducted at Biotoxtech a...
Autores principales: | , , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
KOREAN PHARMACOPUNCTURE INSTITUTE
2013
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4331978/ https://www.ncbi.nlm.nih.gov/pubmed/25780679 http://dx.doi.org/10.3831/KPI.2013.16.027 |
_version_ | 1782357829720473600 |
---|---|
author | Jung, Da-jung Kim, Sung-chul Lee, Hyung-geol Choi, Yoo-min Sin, Min-seop Choi, Seok-woo Hong, Seung-won Song, Beom-yong Kim, Jong-uk Yook, Tae-han |
author_facet | Jung, Da-jung Kim, Sung-chul Lee, Hyung-geol Choi, Yoo-min Sin, Min-seop Choi, Seok-woo Hong, Seung-won Song, Beom-yong Kim, Jong-uk Yook, Tae-han |
author_sort | Jung, Da-jung |
collection | PubMed |
description | OBJECTIVES: This study was performed to analyze the toxicity of the test substance, anti-inflammatory pharmacopuncture (AIP), when used as a single intramuscular-dose in 6-week-old, male and female Sprague-Dawley rats and to find the lethal dose. METHODS: The experiment was conducted at Biotoxtech according to Good Laboratory Practices. Twenty (20) female and 20 male Spague-Dawley rats were divided into 4 groups of five 5 female and 5 male animals per group. The rats in the three experimental groups received single intramuscular injections with 0.1-㎖, 0.5-㎖ and 1.0-㎖/animal doses of AIP, Groups 2, 3, and 4, respectively, and the control group, Group 1, received a single intramuscular injection with a 1.0-㎖ dose of normal saline. Clinical signs were observed and body weight measurements were carried out for 14 days following the injections. At the end of the observation period, hematology, clinical chemistry, histopathological tests and necropsy were performed on the injected parts. RESULTS: No deaths occurred in any of the groups. Also, histopathological tests showed that AIP had no effect on the injected parts in terms of clinical signs, body weight, hematology, clinical chemistry, and necropsy. CONCLUSIONS: As a result of single intramuscular-dose tests of the test substance AIP in 4 groups of rats, the lethal dose for both males and females exceeded 1.0㎖/animal. Therefore, AIP is a relatively safe pharmacopuncture that can be used for treatment, but further studies should be performed. |
format | Online Article Text |
id | pubmed-4331978 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2013 |
publisher | KOREAN PHARMACOPUNCTURE INSTITUTE |
record_format | MEDLINE/PubMed |
spelling | pubmed-43319782015-03-16 Single Intramuscular-dose Toxicity of Anti-inflammatory Pharmacopuncture in Rats Jung, Da-jung Kim, Sung-chul Lee, Hyung-geol Choi, Yoo-min Sin, Min-seop Choi, Seok-woo Hong, Seung-won Song, Beom-yong Kim, Jong-uk Yook, Tae-han J Pharmacopuncture Original Article OBJECTIVES: This study was performed to analyze the toxicity of the test substance, anti-inflammatory pharmacopuncture (AIP), when used as a single intramuscular-dose in 6-week-old, male and female Sprague-Dawley rats and to find the lethal dose. METHODS: The experiment was conducted at Biotoxtech according to Good Laboratory Practices. Twenty (20) female and 20 male Spague-Dawley rats were divided into 4 groups of five 5 female and 5 male animals per group. The rats in the three experimental groups received single intramuscular injections with 0.1-㎖, 0.5-㎖ and 1.0-㎖/animal doses of AIP, Groups 2, 3, and 4, respectively, and the control group, Group 1, received a single intramuscular injection with a 1.0-㎖ dose of normal saline. Clinical signs were observed and body weight measurements were carried out for 14 days following the injections. At the end of the observation period, hematology, clinical chemistry, histopathological tests and necropsy were performed on the injected parts. RESULTS: No deaths occurred in any of the groups. Also, histopathological tests showed that AIP had no effect on the injected parts in terms of clinical signs, body weight, hematology, clinical chemistry, and necropsy. CONCLUSIONS: As a result of single intramuscular-dose tests of the test substance AIP in 4 groups of rats, the lethal dose for both males and females exceeded 1.0㎖/animal. Therefore, AIP is a relatively safe pharmacopuncture that can be used for treatment, but further studies should be performed. KOREAN PHARMACOPUNCTURE INSTITUTE 2013-12 /pmc/articles/PMC4331978/ /pubmed/25780679 http://dx.doi.org/10.3831/KPI.2013.16.027 Text en Copyright ©2013, KOREAN PHARMACOPUNCTURE INSTITUTE http://creativecommons.org/licenses/by-nc/3.0/ This is an Open-Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/) which permits unrestricted noncommercial use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Original Article Jung, Da-jung Kim, Sung-chul Lee, Hyung-geol Choi, Yoo-min Sin, Min-seop Choi, Seok-woo Hong, Seung-won Song, Beom-yong Kim, Jong-uk Yook, Tae-han Single Intramuscular-dose Toxicity of Anti-inflammatory Pharmacopuncture in Rats |
title | Single Intramuscular-dose Toxicity of Anti-inflammatory Pharmacopuncture in Rats |
title_full | Single Intramuscular-dose Toxicity of Anti-inflammatory Pharmacopuncture in Rats |
title_fullStr | Single Intramuscular-dose Toxicity of Anti-inflammatory Pharmacopuncture in Rats |
title_full_unstemmed | Single Intramuscular-dose Toxicity of Anti-inflammatory Pharmacopuncture in Rats |
title_short | Single Intramuscular-dose Toxicity of Anti-inflammatory Pharmacopuncture in Rats |
title_sort | single intramuscular-dose toxicity of anti-inflammatory pharmacopuncture in rats |
topic | Original Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4331978/ https://www.ncbi.nlm.nih.gov/pubmed/25780679 http://dx.doi.org/10.3831/KPI.2013.16.027 |
work_keys_str_mv | AT jungdajung singleintramusculardosetoxicityofantiinflammatorypharmacopunctureinrats AT kimsungchul singleintramusculardosetoxicityofantiinflammatorypharmacopunctureinrats AT leehyunggeol singleintramusculardosetoxicityofantiinflammatorypharmacopunctureinrats AT choiyoomin singleintramusculardosetoxicityofantiinflammatorypharmacopunctureinrats AT sinminseop singleintramusculardosetoxicityofantiinflammatorypharmacopunctureinrats AT choiseokwoo singleintramusculardosetoxicityofantiinflammatorypharmacopunctureinrats AT hongseungwon singleintramusculardosetoxicityofantiinflammatorypharmacopunctureinrats AT songbeomyong singleintramusculardosetoxicityofantiinflammatorypharmacopunctureinrats AT kimjonguk singleintramusculardosetoxicityofantiinflammatorypharmacopunctureinrats AT yooktaehan singleintramusculardosetoxicityofantiinflammatorypharmacopunctureinrats |