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Shengmaisan Regulates Pacemaker Potentials in Interstitial Cells of Cajal in Mice
OBJECTIVES: Shengmaisan (SMS) is a traditional Chinese medicine prescription widely used for the treatment of diverse organs in Korea. The interstitial cells of Cajal (ICCs) are pacemaker cells that play an important role in the generation of coordinated gastrointestinal (GI) motility. We have aimed...
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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KOREAN PHARMACOPUNCTURE INSTITUTE
2013
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4331980/ https://www.ncbi.nlm.nih.gov/pubmed/25780681 http://dx.doi.org/10.3831/KPI.2013.16.025 |
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author | Kim, Byung Joo |
author_facet | Kim, Byung Joo |
author_sort | Kim, Byung Joo |
collection | PubMed |
description | OBJECTIVES: Shengmaisan (SMS) is a traditional Chinese medicine prescription widely used for the treatment of diverse organs in Korea. The interstitial cells of Cajal (ICCs) are pacemaker cells that play an important role in the generation of coordinated gastrointestinal (GI) motility. We have aimed to investigate the effects of SMS in the ICCs in the mouse small intestine. METHODS: To dissociate the ICCs, we used enzymatic digestions from the small intestine in a mouse. After that, the ICCs were identified immunologically by using the anti-c-kit antibody. In the ICCs, the electrophysiological whole-cell patch-clamp configuration was used to record pacemaker potentials in the cultured ICCs. RESULTS: The ICCs generated pacemaker potentials in the mouse small intestine. SMS produced membrane depolarization with concentration-dependent manners in the current clamp mode. Pretreatment with a Ca(2+) free solution and thapsigargin, a Ca(2+)-ATPase inhibitor in the endoplasmic reticulum, stopped the generation of the pacemaker potentials. In the case of Ca(2+)-free solutions, SMS induced membrane depolarizations. However, when thapsigargin in a bath solution was applied, the membrane depolarization was not produced by SMS. The membrane depolarizations produced by SMS were inhibited by U-73122, an active phospholipase C (PLC) inhibitors. Furthermore, chelerythrine and calphostin C, a protein kinase C (PKC) inhibitors had no effects on SMS-induced membrane depolarizations. CONCLUSIONS: These results suggest that SMS might affect GI motility by modulating the pacemaker activity through an internal Ca(2+)- and PLC-dependent and PKC-independent pathway in the ICCs. |
format | Online Article Text |
id | pubmed-4331980 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2013 |
publisher | KOREAN PHARMACOPUNCTURE INSTITUTE |
record_format | MEDLINE/PubMed |
spelling | pubmed-43319802015-03-16 Shengmaisan Regulates Pacemaker Potentials in Interstitial Cells of Cajal in Mice Kim, Byung Joo J Pharmacopuncture Original Article OBJECTIVES: Shengmaisan (SMS) is a traditional Chinese medicine prescription widely used for the treatment of diverse organs in Korea. The interstitial cells of Cajal (ICCs) are pacemaker cells that play an important role in the generation of coordinated gastrointestinal (GI) motility. We have aimed to investigate the effects of SMS in the ICCs in the mouse small intestine. METHODS: To dissociate the ICCs, we used enzymatic digestions from the small intestine in a mouse. After that, the ICCs were identified immunologically by using the anti-c-kit antibody. In the ICCs, the electrophysiological whole-cell patch-clamp configuration was used to record pacemaker potentials in the cultured ICCs. RESULTS: The ICCs generated pacemaker potentials in the mouse small intestine. SMS produced membrane depolarization with concentration-dependent manners in the current clamp mode. Pretreatment with a Ca(2+) free solution and thapsigargin, a Ca(2+)-ATPase inhibitor in the endoplasmic reticulum, stopped the generation of the pacemaker potentials. In the case of Ca(2+)-free solutions, SMS induced membrane depolarizations. However, when thapsigargin in a bath solution was applied, the membrane depolarization was not produced by SMS. The membrane depolarizations produced by SMS were inhibited by U-73122, an active phospholipase C (PLC) inhibitors. Furthermore, chelerythrine and calphostin C, a protein kinase C (PKC) inhibitors had no effects on SMS-induced membrane depolarizations. CONCLUSIONS: These results suggest that SMS might affect GI motility by modulating the pacemaker activity through an internal Ca(2+)- and PLC-dependent and PKC-independent pathway in the ICCs. KOREAN PHARMACOPUNCTURE INSTITUTE 2013-12 /pmc/articles/PMC4331980/ /pubmed/25780681 http://dx.doi.org/10.3831/KPI.2013.16.025 Text en Copyright ©2013, KOREAN PHARMACOPUNCTURE INSTITUTE http://creativecommons.org/licenses/by-nc/3.0/ This is an Open-Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/) which permits unrestricted noncommercial use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Original Article Kim, Byung Joo Shengmaisan Regulates Pacemaker Potentials in Interstitial Cells of Cajal in Mice |
title | Shengmaisan Regulates Pacemaker Potentials in Interstitial Cells of Cajal in Mice |
title_full | Shengmaisan Regulates Pacemaker Potentials in Interstitial Cells of Cajal in Mice |
title_fullStr | Shengmaisan Regulates Pacemaker Potentials in Interstitial Cells of Cajal in Mice |
title_full_unstemmed | Shengmaisan Regulates Pacemaker Potentials in Interstitial Cells of Cajal in Mice |
title_short | Shengmaisan Regulates Pacemaker Potentials in Interstitial Cells of Cajal in Mice |
title_sort | shengmaisan regulates pacemaker potentials in interstitial cells of cajal in mice |
topic | Original Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4331980/ https://www.ncbi.nlm.nih.gov/pubmed/25780681 http://dx.doi.org/10.3831/KPI.2013.16.025 |
work_keys_str_mv | AT kimbyungjoo shengmaisanregulatespacemakerpotentialsininterstitialcellsofcajalinmice |