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Single Intramuscular-dose Toxicity of Water soluble Carthmi-Flos herbal acupuncture (WCF) in Sprague-Dawley Rats

OBJECTIVES: This experiment was conducted to examine the toxicity of Water soluble Carthmi-Flos herbal acupuncture (WCF) by administering a single intramuscular dose of WCF in 6-week-old, male and female Sprague-Dawley rats and to find the lethality dose for WCF. METHODS: The experiment was conducte...

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Autores principales: Lee, Hyung-geol, Kim, Sungchul, Jung, Da-jung, Choi, Yoo-min, Sin, Min-seop, Choi, Seok-Woo, Song, Beom-yong, Kim, Jong-uk, Hong, Seung-won, Yook, Tae-han
Formato: Online Artículo Texto
Lenguaje:English
Publicado: KOREAN PHARMACOPUNCTURE INSTITUTE 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4331990/
https://www.ncbi.nlm.nih.gov/pubmed/25780687
http://dx.doi.org/10.3831/KPI.2014.17.004
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author Lee, Hyung-geol
Kim, Sungchul
Jung, Da-jung
Choi, Yoo-min
Sin, Min-seop
Choi, Seok-Woo
Song, Beom-yong
Kim, Jong-uk
Hong, Seung-won
Yook, Tae-han
author_facet Lee, Hyung-geol
Kim, Sungchul
Jung, Da-jung
Choi, Yoo-min
Sin, Min-seop
Choi, Seok-Woo
Song, Beom-yong
Kim, Jong-uk
Hong, Seung-won
Yook, Tae-han
author_sort Lee, Hyung-geol
collection PubMed
description OBJECTIVES: This experiment was conducted to examine the toxicity of Water soluble Carthmi-Flos herbal acupuncture (WCF) by administering a single intramuscular dose of WCF in 6-week-old, male and female Sprague-Dawley rats and to find the lethality dose for WCF. METHODS: The experiment was conducted at Biotoxtech according to Good Laboratory Practices under a request by the Korean Pharmacopuncture Institute. This experiment was performed based on the testing standards of “Toxicity Test Standards for Drugs” by the Ministry of Food and Drug Safety. Subjects were divided into 4 groups: 1 control group in which normal saline was administered and 3 test groups in which 0.1, 0.5 or 1.0 mL of WCF was administered; a single intramuscular dose was injected into 5 males and 5 females in each group. General symptoms and body weights were observed/measured for 14 days after injection. At the end of the observation period, hematological and clinical chemistry tests were performed, followed by necropsy and histopathological examinations of the injected sections. RESULTS: No mortalities were observed in any group. Also, symptoms, body weight, hematology, clinical chemistry and necropsy were not affected. However, histopathological examination of the injected part in one female in the 1.0-mL group showed infiltration of mononuclear cells and a multi-nucleated giant cell around eosinophilic material. CONCLUSION: Administration of single intramuscular doses of WCF in 3 groups of rats showed that the approximate lethal dose of WCF for all rats was in excess of 1.0 mL, as no mortalities were observed for injections up to and including 1.0 mL.
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spelling pubmed-43319902015-03-16 Single Intramuscular-dose Toxicity of Water soluble Carthmi-Flos herbal acupuncture (WCF) in Sprague-Dawley Rats Lee, Hyung-geol Kim, Sungchul Jung, Da-jung Choi, Yoo-min Sin, Min-seop Choi, Seok-Woo Song, Beom-yong Kim, Jong-uk Hong, Seung-won Yook, Tae-han J Pharmacopuncture Original Article OBJECTIVES: This experiment was conducted to examine the toxicity of Water soluble Carthmi-Flos herbal acupuncture (WCF) by administering a single intramuscular dose of WCF in 6-week-old, male and female Sprague-Dawley rats and to find the lethality dose for WCF. METHODS: The experiment was conducted at Biotoxtech according to Good Laboratory Practices under a request by the Korean Pharmacopuncture Institute. This experiment was performed based on the testing standards of “Toxicity Test Standards for Drugs” by the Ministry of Food and Drug Safety. Subjects were divided into 4 groups: 1 control group in which normal saline was administered and 3 test groups in which 0.1, 0.5 or 1.0 mL of WCF was administered; a single intramuscular dose was injected into 5 males and 5 females in each group. General symptoms and body weights were observed/measured for 14 days after injection. At the end of the observation period, hematological and clinical chemistry tests were performed, followed by necropsy and histopathological examinations of the injected sections. RESULTS: No mortalities were observed in any group. Also, symptoms, body weight, hematology, clinical chemistry and necropsy were not affected. However, histopathological examination of the injected part in one female in the 1.0-mL group showed infiltration of mononuclear cells and a multi-nucleated giant cell around eosinophilic material. CONCLUSION: Administration of single intramuscular doses of WCF in 3 groups of rats showed that the approximate lethal dose of WCF for all rats was in excess of 1.0 mL, as no mortalities were observed for injections up to and including 1.0 mL. KOREAN PHARMACOPUNCTURE INSTITUTE 2014-03 /pmc/articles/PMC4331990/ /pubmed/25780687 http://dx.doi.org/10.3831/KPI.2014.17.004 Text en Copyright ©2014, KOREAN PHARMACOPUNCTURE INSTITUTE http://creativecommons.org/licenses/by-nc/3.0/ This is an Open-Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/) which permits unrestricted noncommercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Article
Lee, Hyung-geol
Kim, Sungchul
Jung, Da-jung
Choi, Yoo-min
Sin, Min-seop
Choi, Seok-Woo
Song, Beom-yong
Kim, Jong-uk
Hong, Seung-won
Yook, Tae-han
Single Intramuscular-dose Toxicity of Water soluble Carthmi-Flos herbal acupuncture (WCF) in Sprague-Dawley Rats
title Single Intramuscular-dose Toxicity of Water soluble Carthmi-Flos herbal acupuncture (WCF) in Sprague-Dawley Rats
title_full Single Intramuscular-dose Toxicity of Water soluble Carthmi-Flos herbal acupuncture (WCF) in Sprague-Dawley Rats
title_fullStr Single Intramuscular-dose Toxicity of Water soluble Carthmi-Flos herbal acupuncture (WCF) in Sprague-Dawley Rats
title_full_unstemmed Single Intramuscular-dose Toxicity of Water soluble Carthmi-Flos herbal acupuncture (WCF) in Sprague-Dawley Rats
title_short Single Intramuscular-dose Toxicity of Water soluble Carthmi-Flos herbal acupuncture (WCF) in Sprague-Dawley Rats
title_sort single intramuscular-dose toxicity of water soluble carthmi-flos herbal acupuncture (wcf) in sprague-dawley rats
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4331990/
https://www.ncbi.nlm.nih.gov/pubmed/25780687
http://dx.doi.org/10.3831/KPI.2014.17.004
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