Isolation and characterization of 22 EST-SSR markers for the genus Thujopsis (Cupressaceae)(1)

• Premise of the study: Expressed sequence tag–simple sequence repeat (EST-SSR) markers were developed from Thujopsis dolabrata var. hondae (Cupressaceae) using Illumina sequencing to investigate the genetic diversity and population structure of the genus Thujopsis. • Methods and Results: Twenty-two...

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Autores principales: Sato, Miyako, Hasegawa, Yoichi, Mishima, Kentaro, Takata, Katsuhiko
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Botanical Society of America 2015
Materias:
Primer Note
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4332144/
https://www.ncbi.nlm.nih.gov/pubmed/25699219
http://dx.doi.org/10.3732/apps.1400101
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author Sato, Miyako
Hasegawa, Yoichi
Mishima, Kentaro
Takata, Katsuhiko
author_facet Sato, Miyako
Hasegawa, Yoichi
Mishima, Kentaro
Takata, Katsuhiko
author_sort Sato, Miyako
collection PubMed
description • Premise of the study: Expressed sequence tag–simple sequence repeat (EST-SSR) markers were developed from Thujopsis dolabrata var. hondae (Cupressaceae) using Illumina sequencing to investigate the genetic diversity and population structure of the genus Thujopsis. • Methods and Results: Twenty-two primer pairs were developed from ESTs of T. dolabrata var. hondae. The primers amplified di- and trinucleotide repeat-containing sequences. Polymorphisms were assessed in 81 individuals from two T. dolabrata var. hondae populations and one T. dolabrata population. The number of alleles ranged from one to 17 per locus. The observed and expected heterozygosities ranged from 0.000 to 1.000 and from 0.000 to 0.926, respectively. • Conclusions: These new EST-SSR markers will be useful in analyses of the genetic diversity and population structure of the genus Thujopsis.
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spelling pubmed-43321442015-02-19 Isolation and characterization of 22 EST-SSR markers for the genus Thujopsis (Cupressaceae)(1) Sato, Miyako Hasegawa, Yoichi Mishima, Kentaro Takata, Katsuhiko Appl Plant Sci Primer Note • Premise of the study: Expressed sequence tag–simple sequence repeat (EST-SSR) markers were developed from Thujopsis dolabrata var. hondae (Cupressaceae) using Illumina sequencing to investigate the genetic diversity and population structure of the genus Thujopsis. • Methods and Results: Twenty-two primer pairs were developed from ESTs of T. dolabrata var. hondae. The primers amplified di- and trinucleotide repeat-containing sequences. Polymorphisms were assessed in 81 individuals from two T. dolabrata var. hondae populations and one T. dolabrata population. The number of alleles ranged from one to 17 per locus. The observed and expected heterozygosities ranged from 0.000 to 1.000 and from 0.000 to 0.926, respectively. • Conclusions: These new EST-SSR markers will be useful in analyses of the genetic diversity and population structure of the genus Thujopsis. Botanical Society of America 2015-01-30 /pmc/articles/PMC4332144/ /pubmed/25699219 http://dx.doi.org/10.3732/apps.1400101 Text en © 2015 Sato et al. Published by the Botanical Society of America http://creativecommons.org/licenses/by-nc/4.0/ This work is licensed under a Creative Commons Attribution License (CC-BY-NC-SA).
spellingShingle Primer Note
Sato, Miyako
Hasegawa, Yoichi
Mishima, Kentaro
Takata, Katsuhiko
Isolation and characterization of 22 EST-SSR markers for the genus Thujopsis (Cupressaceae)(1)
title Isolation and characterization of 22 EST-SSR markers for the genus Thujopsis (Cupressaceae)(1)
title_full Isolation and characterization of 22 EST-SSR markers for the genus Thujopsis (Cupressaceae)(1)
title_fullStr Isolation and characterization of 22 EST-SSR markers for the genus Thujopsis (Cupressaceae)(1)
title_full_unstemmed Isolation and characterization of 22 EST-SSR markers for the genus Thujopsis (Cupressaceae)(1)
title_short Isolation and characterization of 22 EST-SSR markers for the genus Thujopsis (Cupressaceae)(1)
title_sort isolation and characterization of 22 est-ssr markers for the genus thujopsis (cupressaceae)(1)
topic Primer Note
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4332144/
https://www.ncbi.nlm.nih.gov/pubmed/25699219
http://dx.doi.org/10.3732/apps.1400101
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