Cargando…

Thyroid hormone does not induce maturation of embryonic chicken cardiomyocytes in vitro

Fetal cardiac growth in mammalian models occurs primarily by cell proliferation (hyperplasia). However, most cardiomyocytes lose the ability to proliferate close to term and heart growth continues by increasing cell size (hypertrophy). In mammals, the thyroid hormone triiodothyronine (T(3)) is an im...

Descripción completa

Detalles Bibliográficos
Autores principales: Svensson Holm, Ann‐Charlotte B., Lindgren, Isa, Österman, Hanna, Altimiras, Jordi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Wiley Periodicals, Inc. 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4332193/
https://www.ncbi.nlm.nih.gov/pubmed/25501434
http://dx.doi.org/10.14814/phy2.12182
_version_ 1782357866031611904
author Svensson Holm, Ann‐Charlotte B.
Lindgren, Isa
Österman, Hanna
Altimiras, Jordi
author_facet Svensson Holm, Ann‐Charlotte B.
Lindgren, Isa
Österman, Hanna
Altimiras, Jordi
author_sort Svensson Holm, Ann‐Charlotte B.
collection PubMed
description Fetal cardiac growth in mammalian models occurs primarily by cell proliferation (hyperplasia). However, most cardiomyocytes lose the ability to proliferate close to term and heart growth continues by increasing cell size (hypertrophy). In mammals, the thyroid hormone triiodothyronine (T(3)) is an important driver of this process. Chicken cardiomyocytes, however, keep their proliferating ability long after hatching but little information is available on the mechanisms controlling cell growth and myocyte maturation in the chicken heart. Our aim was to study the role of T(3) on proliferation and differentiation of embryonic chicken cardiomyocytes (ECCM), enzymatically isolated from 19‐day‐old embryos and to compare the effects to those of insulin‐like growth factor‐1 (IGF‐1) and phenylephrine (PE). Hyperplasia was measured using a proliferation assay (MTS) and hypertrophy/multinucleation was analyzed morphologically by phalloidin staining of F‐actin and nuclear staining with DAPI. We show that IGF‐1 induces a significant increase in ECCM proliferation (30%) which is absent with T(3) and PE. PE induced both hypertrophy (61%) and multinucleation (41%) but IGF‐1 or T(3) did not. In conclusion, we show that T(3) does not induce maturation or proliferation of cardiomyocytes, while IGF‐1 induces cardiomyocyte proliferation and PE induces maturation of cardiomyocytes.
format Online
Article
Text
id pubmed-4332193
institution National Center for Biotechnology Information
language English
publishDate 2014
publisher Wiley Periodicals, Inc.
record_format MEDLINE/PubMed
spelling pubmed-43321932015-04-07 Thyroid hormone does not induce maturation of embryonic chicken cardiomyocytes in vitro Svensson Holm, Ann‐Charlotte B. Lindgren, Isa Österman, Hanna Altimiras, Jordi Physiol Rep Original Research Fetal cardiac growth in mammalian models occurs primarily by cell proliferation (hyperplasia). However, most cardiomyocytes lose the ability to proliferate close to term and heart growth continues by increasing cell size (hypertrophy). In mammals, the thyroid hormone triiodothyronine (T(3)) is an important driver of this process. Chicken cardiomyocytes, however, keep their proliferating ability long after hatching but little information is available on the mechanisms controlling cell growth and myocyte maturation in the chicken heart. Our aim was to study the role of T(3) on proliferation and differentiation of embryonic chicken cardiomyocytes (ECCM), enzymatically isolated from 19‐day‐old embryos and to compare the effects to those of insulin‐like growth factor‐1 (IGF‐1) and phenylephrine (PE). Hyperplasia was measured using a proliferation assay (MTS) and hypertrophy/multinucleation was analyzed morphologically by phalloidin staining of F‐actin and nuclear staining with DAPI. We show that IGF‐1 induces a significant increase in ECCM proliferation (30%) which is absent with T(3) and PE. PE induced both hypertrophy (61%) and multinucleation (41%) but IGF‐1 or T(3) did not. In conclusion, we show that T(3) does not induce maturation or proliferation of cardiomyocytes, while IGF‐1 induces cardiomyocyte proliferation and PE induces maturation of cardiomyocytes. Wiley Periodicals, Inc. 2014-12-11 /pmc/articles/PMC4332193/ /pubmed/25501434 http://dx.doi.org/10.14814/phy2.12182 Text en © 2014 The Authors. Physiological Reports published by Wiley Periodicals, Inc. on behalf of the American Physiological Society and The Physiological Society. http://creativecommons.org/licenses/by/4.0/ This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Research
Svensson Holm, Ann‐Charlotte B.
Lindgren, Isa
Österman, Hanna
Altimiras, Jordi
Thyroid hormone does not induce maturation of embryonic chicken cardiomyocytes in vitro
title Thyroid hormone does not induce maturation of embryonic chicken cardiomyocytes in vitro
title_full Thyroid hormone does not induce maturation of embryonic chicken cardiomyocytes in vitro
title_fullStr Thyroid hormone does not induce maturation of embryonic chicken cardiomyocytes in vitro
title_full_unstemmed Thyroid hormone does not induce maturation of embryonic chicken cardiomyocytes in vitro
title_short Thyroid hormone does not induce maturation of embryonic chicken cardiomyocytes in vitro
title_sort thyroid hormone does not induce maturation of embryonic chicken cardiomyocytes in vitro
topic Original Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4332193/
https://www.ncbi.nlm.nih.gov/pubmed/25501434
http://dx.doi.org/10.14814/phy2.12182
work_keys_str_mv AT svenssonholmanncharlotteb thyroidhormonedoesnotinducematurationofembryonicchickencardiomyocytesinvitro
AT lindgrenisa thyroidhormonedoesnotinducematurationofembryonicchickencardiomyocytesinvitro
AT ostermanhanna thyroidhormonedoesnotinducematurationofembryonicchickencardiomyocytesinvitro
AT altimirasjordi thyroidhormonedoesnotinducematurationofembryonicchickencardiomyocytesinvitro