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Loading‐associated expression of TRIM72 and caveolin‐3 in antigravitational soleus muscle in mice

Effects of mechanical loading on the expression level of tripartite motif‐containing 72 (TRIM72) and caveolin‐3 (Cav‐3) in mouse soleus muscle were investigated. Mice were subjected to (1) continuous hindlimb suspension (HS) for 2 weeks followed by 1‐week ambulation recovery or (2) functional overlo...

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Autores principales: Ohno, Yoshitaka, Sugiura, Takao, Ohira, Yoshinobu, Yoshioka, Toshitada, Goto, Katsumasa
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Wiley Periodicals, Inc. 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4332229/
https://www.ncbi.nlm.nih.gov/pubmed/25539835
http://dx.doi.org/10.14814/phy2.12259
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author Ohno, Yoshitaka
Sugiura, Takao
Ohira, Yoshinobu
Yoshioka, Toshitada
Goto, Katsumasa
author_facet Ohno, Yoshitaka
Sugiura, Takao
Ohira, Yoshinobu
Yoshioka, Toshitada
Goto, Katsumasa
author_sort Ohno, Yoshitaka
collection PubMed
description Effects of mechanical loading on the expression level of tripartite motif‐containing 72 (TRIM72) and caveolin‐3 (Cav‐3) in mouse soleus muscle were investigated. Mice were subjected to (1) continuous hindlimb suspension (HS) for 2 weeks followed by 1‐week ambulation recovery or (2) functional overloading (FO) on the soleus by cutting the distal tendons of the plantaris and gastrocnemius muscles. Soleus muscle atrophy was induced by 2‐week hindlimb suspension (HS). Reloading‐associated regrowth of atrophied soleus muscle was observed by 1‐week reloading following HS. HS also depressed the expression level of insulin receptor substrate‐1 (IRS‐1) mRNA, TRIM72, Cav‐3, and phosphorylated Akt (p‐Akt)/total Akt (t‐Akt), but increased the phosphorylated level of p38 mitogen‐activated protein kinase (p‐p38MAPK) in soleus muscle. Thereafter, the expression level of MyoD mRNA, TRIM72 (mRNA, and protein), and Cav‐3 was significantly increased and recovered to the basal level during 1‐week reloading after HS. Although IRS‐1 expression was also upregulated by reloading, the expression level was significantly lower than that before HS. Significant increase in p‐Akt and phosphorylated p70 S6 kinase (p‐p70S6K) was observed by 1‐day reloading. On the other hand, 1‐week functional overloading (FO) induced soleus muscle hypertrophy. In FO‐associated hypertrophied soleus muscle, the expression level of IRS‐1 mRNA, MyoD mRNA, TRIM72 mRNA, p‐Akt, and p‐p70S6K was increased, but the expression of Cav‐3 and p‐p38MAPK was decreased. FO had no effect on the protein expression level of TRIM72. These observations suggest that the loading‐associated upregulation of TRIM72 protein in skeletal muscle may depress the regrowth of atrophied muscle via a partial suppression of IRS‐1. In addition, downregulation of Cav‐3 in skeletal muscle may depress overloading‐induced muscle hypertrophy.
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spelling pubmed-43322292015-04-07 Loading‐associated expression of TRIM72 and caveolin‐3 in antigravitational soleus muscle in mice Ohno, Yoshitaka Sugiura, Takao Ohira, Yoshinobu Yoshioka, Toshitada Goto, Katsumasa Physiol Rep Original Research Effects of mechanical loading on the expression level of tripartite motif‐containing 72 (TRIM72) and caveolin‐3 (Cav‐3) in mouse soleus muscle were investigated. Mice were subjected to (1) continuous hindlimb suspension (HS) for 2 weeks followed by 1‐week ambulation recovery or (2) functional overloading (FO) on the soleus by cutting the distal tendons of the plantaris and gastrocnemius muscles. Soleus muscle atrophy was induced by 2‐week hindlimb suspension (HS). Reloading‐associated regrowth of atrophied soleus muscle was observed by 1‐week reloading following HS. HS also depressed the expression level of insulin receptor substrate‐1 (IRS‐1) mRNA, TRIM72, Cav‐3, and phosphorylated Akt (p‐Akt)/total Akt (t‐Akt), but increased the phosphorylated level of p38 mitogen‐activated protein kinase (p‐p38MAPK) in soleus muscle. Thereafter, the expression level of MyoD mRNA, TRIM72 (mRNA, and protein), and Cav‐3 was significantly increased and recovered to the basal level during 1‐week reloading after HS. Although IRS‐1 expression was also upregulated by reloading, the expression level was significantly lower than that before HS. Significant increase in p‐Akt and phosphorylated p70 S6 kinase (p‐p70S6K) was observed by 1‐day reloading. On the other hand, 1‐week functional overloading (FO) induced soleus muscle hypertrophy. In FO‐associated hypertrophied soleus muscle, the expression level of IRS‐1 mRNA, MyoD mRNA, TRIM72 mRNA, p‐Akt, and p‐p70S6K was increased, but the expression of Cav‐3 and p‐p38MAPK was decreased. FO had no effect on the protein expression level of TRIM72. These observations suggest that the loading‐associated upregulation of TRIM72 protein in skeletal muscle may depress the regrowth of atrophied muscle via a partial suppression of IRS‐1. In addition, downregulation of Cav‐3 in skeletal muscle may depress overloading‐induced muscle hypertrophy. Wiley Periodicals, Inc. 2014-12-24 /pmc/articles/PMC4332229/ /pubmed/25539835 http://dx.doi.org/10.14814/phy2.12259 Text en © 2014 The Authors. Physiological Reports published by Wiley Periodicals, Inc. on behalf of the American Physiological Society and The Physiological Society. http://creativecommons.org/licenses/by/4.0/ This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Research
Ohno, Yoshitaka
Sugiura, Takao
Ohira, Yoshinobu
Yoshioka, Toshitada
Goto, Katsumasa
Loading‐associated expression of TRIM72 and caveolin‐3 in antigravitational soleus muscle in mice
title Loading‐associated expression of TRIM72 and caveolin‐3 in antigravitational soleus muscle in mice
title_full Loading‐associated expression of TRIM72 and caveolin‐3 in antigravitational soleus muscle in mice
title_fullStr Loading‐associated expression of TRIM72 and caveolin‐3 in antigravitational soleus muscle in mice
title_full_unstemmed Loading‐associated expression of TRIM72 and caveolin‐3 in antigravitational soleus muscle in mice
title_short Loading‐associated expression of TRIM72 and caveolin‐3 in antigravitational soleus muscle in mice
title_sort loading‐associated expression of trim72 and caveolin‐3 in antigravitational soleus muscle in mice
topic Original Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4332229/
https://www.ncbi.nlm.nih.gov/pubmed/25539835
http://dx.doi.org/10.14814/phy2.12259
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