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A metagenomic approach to characterize temperate bacteriophage populations from Cystic Fibrosis and non-Cystic Fibrosis bronchiectasis patients
Pseudomonas aeruginosa (Pa), normally a soil commensal, is an important opportunistic pathogen in Cystic Fibrosis (CF) and non-Cystic Fibrosis Bronchiectasis (nCFBR). Persistent infection correlates with accelerated decline in lung function and early mortality. The horizontal transfer of DNA by temp...
Autores principales: | , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Frontiers Media S.A.
2015
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4332376/ https://www.ncbi.nlm.nih.gov/pubmed/25741327 http://dx.doi.org/10.3389/fmicb.2015.00097 |
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author | Tariq, Mohammad A. Everest, Francesca L. C. Cowley, Lauren A. De Soyza, Anthony Holt, Giles S. Bridge, Simon H. Perry, Audrey Perry, John D. Bourke, Stephen J. Cummings, Stephen P. Lanyon, Clare V. Barr, Jeremy J. Smith, Darren L. |
author_facet | Tariq, Mohammad A. Everest, Francesca L. C. Cowley, Lauren A. De Soyza, Anthony Holt, Giles S. Bridge, Simon H. Perry, Audrey Perry, John D. Bourke, Stephen J. Cummings, Stephen P. Lanyon, Clare V. Barr, Jeremy J. Smith, Darren L. |
author_sort | Tariq, Mohammad A. |
collection | PubMed |
description | Pseudomonas aeruginosa (Pa), normally a soil commensal, is an important opportunistic pathogen in Cystic Fibrosis (CF) and non-Cystic Fibrosis Bronchiectasis (nCFBR). Persistent infection correlates with accelerated decline in lung function and early mortality. The horizontal transfer of DNA by temperate bacteriophages can add gene function and selective advantages to their bacterial host within the constrained environment of the lower lung. In this study, we chemically induce temperate bacteriophages from clonal cultures of Pa and identify their mixed viral communities employing metagenomic approaches. We compared 92 temperate phage metagenomes stratified from these clinical backgrounds (47 CF and 45 nCFBR Pa isolates) using MG-RAST and GeneWise2. KEGG analysis shows the complexity of temperate phage accessory gene carriage increases with duration and severity of the disease. Furthermore, we identify the presence of Ig-like motifs within phage structural genes linked to bacterial adhesion and carbohydrate binding including Big_2, He_Pig, and Fn3. This study provides the first clinical support to the proposed bacteriophage adherence to mucus (BAM) model and the evolution of phages interacting at these mucosal surfaces over time. |
format | Online Article Text |
id | pubmed-4332376 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2015 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-43323762015-03-04 A metagenomic approach to characterize temperate bacteriophage populations from Cystic Fibrosis and non-Cystic Fibrosis bronchiectasis patients Tariq, Mohammad A. Everest, Francesca L. C. Cowley, Lauren A. De Soyza, Anthony Holt, Giles S. Bridge, Simon H. Perry, Audrey Perry, John D. Bourke, Stephen J. Cummings, Stephen P. Lanyon, Clare V. Barr, Jeremy J. Smith, Darren L. Front Microbiol Microbiology Pseudomonas aeruginosa (Pa), normally a soil commensal, is an important opportunistic pathogen in Cystic Fibrosis (CF) and non-Cystic Fibrosis Bronchiectasis (nCFBR). Persistent infection correlates with accelerated decline in lung function and early mortality. The horizontal transfer of DNA by temperate bacteriophages can add gene function and selective advantages to their bacterial host within the constrained environment of the lower lung. In this study, we chemically induce temperate bacteriophages from clonal cultures of Pa and identify their mixed viral communities employing metagenomic approaches. We compared 92 temperate phage metagenomes stratified from these clinical backgrounds (47 CF and 45 nCFBR Pa isolates) using MG-RAST and GeneWise2. KEGG analysis shows the complexity of temperate phage accessory gene carriage increases with duration and severity of the disease. Furthermore, we identify the presence of Ig-like motifs within phage structural genes linked to bacterial adhesion and carbohydrate binding including Big_2, He_Pig, and Fn3. This study provides the first clinical support to the proposed bacteriophage adherence to mucus (BAM) model and the evolution of phages interacting at these mucosal surfaces over time. Frontiers Media S.A. 2015-02-18 /pmc/articles/PMC4332376/ /pubmed/25741327 http://dx.doi.org/10.3389/fmicb.2015.00097 Text en Copyright © 2015 Tariq, Everest, Cowley, De Soyza, Holt, Bridge, Perry, Perry, Bourke, Cummings, Lanyon, Barr and Smith. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Microbiology Tariq, Mohammad A. Everest, Francesca L. C. Cowley, Lauren A. De Soyza, Anthony Holt, Giles S. Bridge, Simon H. Perry, Audrey Perry, John D. Bourke, Stephen J. Cummings, Stephen P. Lanyon, Clare V. Barr, Jeremy J. Smith, Darren L. A metagenomic approach to characterize temperate bacteriophage populations from Cystic Fibrosis and non-Cystic Fibrosis bronchiectasis patients |
title | A metagenomic approach to characterize temperate bacteriophage populations from Cystic Fibrosis and non-Cystic Fibrosis bronchiectasis patients |
title_full | A metagenomic approach to characterize temperate bacteriophage populations from Cystic Fibrosis and non-Cystic Fibrosis bronchiectasis patients |
title_fullStr | A metagenomic approach to characterize temperate bacteriophage populations from Cystic Fibrosis and non-Cystic Fibrosis bronchiectasis patients |
title_full_unstemmed | A metagenomic approach to characterize temperate bacteriophage populations from Cystic Fibrosis and non-Cystic Fibrosis bronchiectasis patients |
title_short | A metagenomic approach to characterize temperate bacteriophage populations from Cystic Fibrosis and non-Cystic Fibrosis bronchiectasis patients |
title_sort | metagenomic approach to characterize temperate bacteriophage populations from cystic fibrosis and non-cystic fibrosis bronchiectasis patients |
topic | Microbiology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4332376/ https://www.ncbi.nlm.nih.gov/pubmed/25741327 http://dx.doi.org/10.3389/fmicb.2015.00097 |
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