Cargando…
Antigen Targeting to Dendritic Cells Allows the Identification of a CD4 T-Cell Epitope within an Immunodominant Trypanosoma cruzi Antigen
Targeting antigens to dendritic cells (DCs) by using hybrid monoclonal antibodies (mAbs) directed against DC receptors is known to improve activation and support long-lasting T cell responses. In the present work, we used the mAb αDEC205 fused to the Trypanosoma cruzi amastigote surface protein 2 (A...
Autores principales: | , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2015
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4332658/ https://www.ncbi.nlm.nih.gov/pubmed/25679777 http://dx.doi.org/10.1371/journal.pone.0117778 |
_version_ | 1782357936229580800 |
---|---|
author | Rampazo, Eline V. Amorim, Kelly N. S. Yamamoto, Marcio M. Panatieri, Raquel Hoffmann Rodrigues, Mauricio M. Boscardin, Silvia B. |
author_facet | Rampazo, Eline V. Amorim, Kelly N. S. Yamamoto, Marcio M. Panatieri, Raquel Hoffmann Rodrigues, Mauricio M. Boscardin, Silvia B. |
author_sort | Rampazo, Eline V. |
collection | PubMed |
description | Targeting antigens to dendritic cells (DCs) by using hybrid monoclonal antibodies (mAbs) directed against DC receptors is known to improve activation and support long-lasting T cell responses. In the present work, we used the mAb αDEC205 fused to the Trypanosoma cruzi amastigote surface protein 2 (ASP-2) to identify a region of this protein recognized by specific T cells. The hybrid αDEC-ASP2 mAb was successfully generated and preserved its ability to bind the DEC205 receptor. Immunization of BALB/c mice with the recombinant mAb in the presence of polyriboinosinic: polyribocytidylic acid (poly (I:C)) specifically enhanced the number of IFN-γ producing cells and CD4+ T cell proliferation when compared to mice immunized with a mAb without receptor affinity or with the non-targeted ASP-2 protein. The strong immune response induced in mice immunized with the hybrid αDEC-ASP2 mAb allowed us to identify an ASP-2-specific CD4+ T cell epitope recognized by the BALB/c MHCII haplotype. We conclude that targeting parasite antigens to DCs is a useful strategy to enhance T cell mediated immune responses facilitating the identification of new T-cell epitopes. |
format | Online Article Text |
id | pubmed-4332658 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2015 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-43326582015-02-24 Antigen Targeting to Dendritic Cells Allows the Identification of a CD4 T-Cell Epitope within an Immunodominant Trypanosoma cruzi Antigen Rampazo, Eline V. Amorim, Kelly N. S. Yamamoto, Marcio M. Panatieri, Raquel Hoffmann Rodrigues, Mauricio M. Boscardin, Silvia B. PLoS One Research Article Targeting antigens to dendritic cells (DCs) by using hybrid monoclonal antibodies (mAbs) directed against DC receptors is known to improve activation and support long-lasting T cell responses. In the present work, we used the mAb αDEC205 fused to the Trypanosoma cruzi amastigote surface protein 2 (ASP-2) to identify a region of this protein recognized by specific T cells. The hybrid αDEC-ASP2 mAb was successfully generated and preserved its ability to bind the DEC205 receptor. Immunization of BALB/c mice with the recombinant mAb in the presence of polyriboinosinic: polyribocytidylic acid (poly (I:C)) specifically enhanced the number of IFN-γ producing cells and CD4+ T cell proliferation when compared to mice immunized with a mAb without receptor affinity or with the non-targeted ASP-2 protein. The strong immune response induced in mice immunized with the hybrid αDEC-ASP2 mAb allowed us to identify an ASP-2-specific CD4+ T cell epitope recognized by the BALB/c MHCII haplotype. We conclude that targeting parasite antigens to DCs is a useful strategy to enhance T cell mediated immune responses facilitating the identification of new T-cell epitopes. Public Library of Science 2015-02-13 /pmc/articles/PMC4332658/ /pubmed/25679777 http://dx.doi.org/10.1371/journal.pone.0117778 Text en © 2015 Rampazo et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited. |
spellingShingle | Research Article Rampazo, Eline V. Amorim, Kelly N. S. Yamamoto, Marcio M. Panatieri, Raquel Hoffmann Rodrigues, Mauricio M. Boscardin, Silvia B. Antigen Targeting to Dendritic Cells Allows the Identification of a CD4 T-Cell Epitope within an Immunodominant Trypanosoma cruzi Antigen |
title | Antigen Targeting to Dendritic Cells Allows the Identification of a CD4 T-Cell Epitope within an Immunodominant Trypanosoma cruzi Antigen |
title_full | Antigen Targeting to Dendritic Cells Allows the Identification of a CD4 T-Cell Epitope within an Immunodominant Trypanosoma cruzi Antigen |
title_fullStr | Antigen Targeting to Dendritic Cells Allows the Identification of a CD4 T-Cell Epitope within an Immunodominant Trypanosoma cruzi Antigen |
title_full_unstemmed | Antigen Targeting to Dendritic Cells Allows the Identification of a CD4 T-Cell Epitope within an Immunodominant Trypanosoma cruzi Antigen |
title_short | Antigen Targeting to Dendritic Cells Allows the Identification of a CD4 T-Cell Epitope within an Immunodominant Trypanosoma cruzi Antigen |
title_sort | antigen targeting to dendritic cells allows the identification of a cd4 t-cell epitope within an immunodominant trypanosoma cruzi antigen |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4332658/ https://www.ncbi.nlm.nih.gov/pubmed/25679777 http://dx.doi.org/10.1371/journal.pone.0117778 |
work_keys_str_mv | AT rampazoelinev antigentargetingtodendriticcellsallowstheidentificationofacd4tcellepitopewithinanimmunodominanttrypanosomacruziantigen AT amorimkellyns antigentargetingtodendriticcellsallowstheidentificationofacd4tcellepitopewithinanimmunodominanttrypanosomacruziantigen AT yamamotomarciom antigentargetingtodendriticcellsallowstheidentificationofacd4tcellepitopewithinanimmunodominanttrypanosomacruziantigen AT panatieriraquelhoffmann antigentargetingtodendriticcellsallowstheidentificationofacd4tcellepitopewithinanimmunodominanttrypanosomacruziantigen AT rodriguesmauriciom antigentargetingtodendriticcellsallowstheidentificationofacd4tcellepitopewithinanimmunodominanttrypanosomacruziantigen AT boscardinsilviab antigentargetingtodendriticcellsallowstheidentificationofacd4tcellepitopewithinanimmunodominanttrypanosomacruziantigen |