Bactobolin A Binds to a Site on the 70S Ribosome Distinct from Previously Seen Antibiotics

The ribosome is the target of a large number of antibiotics. Here, we report a 3.4-Å-resolution crystal structure of bactobolin A bound to 70S ribosome–tRNA complex. The antibiotic binds at a previously unseen site in the 50S subunit and displaces tRNA bound at the P-site. It thus likely has a simil...

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Detalles Bibliográficos
Autores principales: Amunts, Alexey, Fiedorczuk, Karol, Truong, Thao T., Chandler, Josephine, Peter Greenberg, E., Ramakrishnan, V.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2015
Materias:
Brevia
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4332689/
https://www.ncbi.nlm.nih.gov/pubmed/25562208
http://dx.doi.org/10.1016/j.jmb.2014.12.018
Descripción
Sumario:The ribosome is the target of a large number of antibiotics. Here, we report a 3.4-Å-resolution crystal structure of bactobolin A bound to 70S ribosome–tRNA complex. The antibiotic binds at a previously unseen site in the 50S subunit and displaces tRNA bound at the P-site. It thus likely has a similar mechanism of action as blasticidin S despite binding to a different site. The structure also rationalizes previously identified resistance mutations.

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