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Impaired Translocation of GLUT4 Results in Insulin Resistance of Atrophic Soleus Muscle
Whether or not the atrophic skeletal muscle induces insulin resistance and its mechanisms are not resolved now. The antigravity soleus muscle showed a progressive atrophy in 1-week, 2-week, and 4-week tail-suspended rats. Hyperinsulinemic-euglycemic clamp showed that the steady-state glucose infusio...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Hindawi Publishing Corporation
2015
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4332754/ https://www.ncbi.nlm.nih.gov/pubmed/25713812 http://dx.doi.org/10.1155/2015/291987 |
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author | Xu, Peng-Tao Song, Zhen Zhang, Wen-Cheng Jiao, Bo Yu, Zhi-Bin |
author_facet | Xu, Peng-Tao Song, Zhen Zhang, Wen-Cheng Jiao, Bo Yu, Zhi-Bin |
author_sort | Xu, Peng-Tao |
collection | PubMed |
description | Whether or not the atrophic skeletal muscle induces insulin resistance and its mechanisms are not resolved now. The antigravity soleus muscle showed a progressive atrophy in 1-week, 2-week, and 4-week tail-suspended rats. Hyperinsulinemic-euglycemic clamp showed that the steady-state glucose infusion rate was lower in 4-week tail-suspended rats than that in the control rats. The glucose uptake rates under insulin- or contraction-stimulation were significantly decreased in 4-week unloaded soleus muscle. The key protein expressions of IRS-1, PI3K, and Akt on the insulin-dependent pathway and of AMPK, ERK, and p38 on the insulin-independent pathway were unchanged in unloaded soleus muscle. The unchanged phosphorylation of Akt and p38 suggested that the activity of two signal pathways was not altered in unloaded soleus muscle. The AS160 and GLUT4 expression on the common downstream pathway also was not changed in unloaded soleus muscle. But the GLUT4 translocation to sarcolemma was inhibited during insulin stimulation in unloaded soleus muscle. The above results suggest that hindlimb unloading in tail-suspended rat induces atrophy in antigravity soleus muscle. The impaired GLUT4 translocation to sarcolemma under insulin stimulation may mediate insulin resistance in unloaded soleus muscle and further affect the insulin sensitivity of whole body in tail-suspended rats. |
format | Online Article Text |
id | pubmed-4332754 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2015 |
publisher | Hindawi Publishing Corporation |
record_format | MEDLINE/PubMed |
spelling | pubmed-43327542015-02-24 Impaired Translocation of GLUT4 Results in Insulin Resistance of Atrophic Soleus Muscle Xu, Peng-Tao Song, Zhen Zhang, Wen-Cheng Jiao, Bo Yu, Zhi-Bin Biomed Res Int Research Article Whether or not the atrophic skeletal muscle induces insulin resistance and its mechanisms are not resolved now. The antigravity soleus muscle showed a progressive atrophy in 1-week, 2-week, and 4-week tail-suspended rats. Hyperinsulinemic-euglycemic clamp showed that the steady-state glucose infusion rate was lower in 4-week tail-suspended rats than that in the control rats. The glucose uptake rates under insulin- or contraction-stimulation were significantly decreased in 4-week unloaded soleus muscle. The key protein expressions of IRS-1, PI3K, and Akt on the insulin-dependent pathway and of AMPK, ERK, and p38 on the insulin-independent pathway were unchanged in unloaded soleus muscle. The unchanged phosphorylation of Akt and p38 suggested that the activity of two signal pathways was not altered in unloaded soleus muscle. The AS160 and GLUT4 expression on the common downstream pathway also was not changed in unloaded soleus muscle. But the GLUT4 translocation to sarcolemma was inhibited during insulin stimulation in unloaded soleus muscle. The above results suggest that hindlimb unloading in tail-suspended rat induces atrophy in antigravity soleus muscle. The impaired GLUT4 translocation to sarcolemma under insulin stimulation may mediate insulin resistance in unloaded soleus muscle and further affect the insulin sensitivity of whole body in tail-suspended rats. Hindawi Publishing Corporation 2015 2015-02-03 /pmc/articles/PMC4332754/ /pubmed/25713812 http://dx.doi.org/10.1155/2015/291987 Text en Copyright © 2015 Peng-Tao Xu et al. https://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Article Xu, Peng-Tao Song, Zhen Zhang, Wen-Cheng Jiao, Bo Yu, Zhi-Bin Impaired Translocation of GLUT4 Results in Insulin Resistance of Atrophic Soleus Muscle |
title | Impaired Translocation of GLUT4 Results in Insulin Resistance of Atrophic Soleus Muscle |
title_full | Impaired Translocation of GLUT4 Results in Insulin Resistance of Atrophic Soleus Muscle |
title_fullStr | Impaired Translocation of GLUT4 Results in Insulin Resistance of Atrophic Soleus Muscle |
title_full_unstemmed | Impaired Translocation of GLUT4 Results in Insulin Resistance of Atrophic Soleus Muscle |
title_short | Impaired Translocation of GLUT4 Results in Insulin Resistance of Atrophic Soleus Muscle |
title_sort | impaired translocation of glut4 results in insulin resistance of atrophic soleus muscle |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4332754/ https://www.ncbi.nlm.nih.gov/pubmed/25713812 http://dx.doi.org/10.1155/2015/291987 |
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