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Impaired Translocation of GLUT4 Results in Insulin Resistance of Atrophic Soleus Muscle

Whether or not the atrophic skeletal muscle induces insulin resistance and its mechanisms are not resolved now. The antigravity soleus muscle showed a progressive atrophy in 1-week, 2-week, and 4-week tail-suspended rats. Hyperinsulinemic-euglycemic clamp showed that the steady-state glucose infusio...

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Autores principales: Xu, Peng-Tao, Song, Zhen, Zhang, Wen-Cheng, Jiao, Bo, Yu, Zhi-Bin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi Publishing Corporation 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4332754/
https://www.ncbi.nlm.nih.gov/pubmed/25713812
http://dx.doi.org/10.1155/2015/291987
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author Xu, Peng-Tao
Song, Zhen
Zhang, Wen-Cheng
Jiao, Bo
Yu, Zhi-Bin
author_facet Xu, Peng-Tao
Song, Zhen
Zhang, Wen-Cheng
Jiao, Bo
Yu, Zhi-Bin
author_sort Xu, Peng-Tao
collection PubMed
description Whether or not the atrophic skeletal muscle induces insulin resistance and its mechanisms are not resolved now. The antigravity soleus muscle showed a progressive atrophy in 1-week, 2-week, and 4-week tail-suspended rats. Hyperinsulinemic-euglycemic clamp showed that the steady-state glucose infusion rate was lower in 4-week tail-suspended rats than that in the control rats. The glucose uptake rates under insulin- or contraction-stimulation were significantly decreased in 4-week unloaded soleus muscle. The key protein expressions of IRS-1, PI3K, and Akt on the insulin-dependent pathway and of AMPK, ERK, and p38 on the insulin-independent pathway were unchanged in unloaded soleus muscle. The unchanged phosphorylation of Akt and p38 suggested that the activity of two signal pathways was not altered in unloaded soleus muscle. The AS160 and GLUT4 expression on the common downstream pathway also was not changed in unloaded soleus muscle. But the GLUT4 translocation to sarcolemma was inhibited during insulin stimulation in unloaded soleus muscle. The above results suggest that hindlimb unloading in tail-suspended rat induces atrophy in antigravity soleus muscle. The impaired GLUT4 translocation to sarcolemma under insulin stimulation may mediate insulin resistance in unloaded soleus muscle and further affect the insulin sensitivity of whole body in tail-suspended rats.
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spelling pubmed-43327542015-02-24 Impaired Translocation of GLUT4 Results in Insulin Resistance of Atrophic Soleus Muscle Xu, Peng-Tao Song, Zhen Zhang, Wen-Cheng Jiao, Bo Yu, Zhi-Bin Biomed Res Int Research Article Whether or not the atrophic skeletal muscle induces insulin resistance and its mechanisms are not resolved now. The antigravity soleus muscle showed a progressive atrophy in 1-week, 2-week, and 4-week tail-suspended rats. Hyperinsulinemic-euglycemic clamp showed that the steady-state glucose infusion rate was lower in 4-week tail-suspended rats than that in the control rats. The glucose uptake rates under insulin- or contraction-stimulation were significantly decreased in 4-week unloaded soleus muscle. The key protein expressions of IRS-1, PI3K, and Akt on the insulin-dependent pathway and of AMPK, ERK, and p38 on the insulin-independent pathway were unchanged in unloaded soleus muscle. The unchanged phosphorylation of Akt and p38 suggested that the activity of two signal pathways was not altered in unloaded soleus muscle. The AS160 and GLUT4 expression on the common downstream pathway also was not changed in unloaded soleus muscle. But the GLUT4 translocation to sarcolemma was inhibited during insulin stimulation in unloaded soleus muscle. The above results suggest that hindlimb unloading in tail-suspended rat induces atrophy in antigravity soleus muscle. The impaired GLUT4 translocation to sarcolemma under insulin stimulation may mediate insulin resistance in unloaded soleus muscle and further affect the insulin sensitivity of whole body in tail-suspended rats. Hindawi Publishing Corporation 2015 2015-02-03 /pmc/articles/PMC4332754/ /pubmed/25713812 http://dx.doi.org/10.1155/2015/291987 Text en Copyright © 2015 Peng-Tao Xu et al. https://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Xu, Peng-Tao
Song, Zhen
Zhang, Wen-Cheng
Jiao, Bo
Yu, Zhi-Bin
Impaired Translocation of GLUT4 Results in Insulin Resistance of Atrophic Soleus Muscle
title Impaired Translocation of GLUT4 Results in Insulin Resistance of Atrophic Soleus Muscle
title_full Impaired Translocation of GLUT4 Results in Insulin Resistance of Atrophic Soleus Muscle
title_fullStr Impaired Translocation of GLUT4 Results in Insulin Resistance of Atrophic Soleus Muscle
title_full_unstemmed Impaired Translocation of GLUT4 Results in Insulin Resistance of Atrophic Soleus Muscle
title_short Impaired Translocation of GLUT4 Results in Insulin Resistance of Atrophic Soleus Muscle
title_sort impaired translocation of glut4 results in insulin resistance of atrophic soleus muscle
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4332754/
https://www.ncbi.nlm.nih.gov/pubmed/25713812
http://dx.doi.org/10.1155/2015/291987
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