New insights into the QuikChange(TM) process guide the use of Phusion DNA polymerase for site-directed mutagenesis

The QuikChange(TM) site-directed mutagenesis method is popular but imperfect. An improvement by using partially overlapping primers has been reported several times; however, it is incompatible with the proposed mechanism. The QuikChange(TM) method using complementary primers is proposed to linearly...

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Detalles Bibliográficos
Autores principales: Xia, Yongzhen, Chu, Wenqiao, Qi, Qingsheng, Xun, Luying
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2015
Materias:
Methods Online
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4333370/
https://www.ncbi.nlm.nih.gov/pubmed/25399421
http://dx.doi.org/10.1093/nar/gku1189

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4333370/
https://www.ncbi.nlm.nih.gov/pubmed/25399421
http://dx.doi.org/10.1093/nar/gku1189

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