Cargando…

New insights into the QuikChange(TM) process guide the use of Phusion DNA polymerase for site-directed mutagenesis

The QuikChange(TM) site-directed mutagenesis method is popular but imperfect. An improvement by using partially overlapping primers has been reported several times; however, it is incompatible with the proposed mechanism. The QuikChange(TM) method using complementary primers is proposed to linearly...

Descripción completa

Detalles Bibliográficos
Autores principales:	Xia, Yongzhen, Chu, Wenqiao, Qi, Qingsheng, Xun, Luying
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Oxford University Press 2015
Materias:	Methods Online
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4333370/ https://www.ncbi.nlm.nih.gov/pubmed/25399421 http://dx.doi.org/10.1093/nar/gku1189

Ejemplares similares

T5 exonuclease-dependent assembly offers a low-cost method for efficient cloning and site-directed mutagenesis
por: Xia, Yongzhen, et al.
Publicado: (2019)

Darwin Assembly: fast, efficient, multi-site bespoke mutagenesis
por: Cozens, Christopher, et al.
Publicado: (2018)

A novel PCR strategy for high-efficiency, automated site-directed mutagenesis
por: Wu, Wu, et al.
Publicado: (2005)

Development of a novel site-specific mutagenesis assay using MALDI-ToF MS (SSMA-MS)
por: McLuckie, Keith I. E., et al.
Publicado: (2006)

Mutagenesis of diploid mammalian genes by gene entrapment
por: Lin, Qing, et al.
Publicado: (2006)

Computational identification of insertional mutagenesis targets for cancer gene discovery
por: de Jong, Johann, et al.
Publicado: (2011)

Targeted insertional mutagenesis libraries for deep domain insertion profiling
por: Coyote-Maestas, Willow, et al.
Publicado: (2020)

Towards systematic identification of Plasmodium essential genes by transposon shuttle mutagenesis
por: Sakamoto, Hiroshi, et al.
Publicado: (2005)

Restriction enzyme-free mutagenesis via the light regulation of DNA polymerization
por: Young, Douglas D., et al.
Publicado: (2009)

Harnessing mutagenic homologous recombination for targeted mutagenesis in vivo by TaGTEAM
por: Finney-Manchester, Shawn P., et al.
Publicado: (2013)

Gene-specific mutagenesis enables rapid continuous evolution of enzymes in vivo
por: Park, Hyojin, et al.
Publicado: (2021)

Targeted de novo phasing and long-range assembly by template mutagenesis
por: Li, Siran, et al.
Publicado: (2022)

Characterization of zebrafish Rad52 and replication protein A for oligonucleotide-mediated mutagenesis
por: Takahashi, Nobuhiro, et al.
Publicado: (2005)

Circular permutation profiling by deep sequencing libraries created using transposon mutagenesis
por: Atkinson, Joshua T, et al.
Publicado: (2018)

Noncanonical amino acid mutagenesis in response to recoding signal-enhanced quadruplet codons
por: Chen, Yan, et al.
Publicado: (2022)

TrimerDimer: an oligonucleotide-based saturation mutagenesis approach that removes redundant and stop codons
por: Gaytán, Paul, et al.
Publicado: (2009)

A piggyBac transposon-based mutagenesis system for the fission yeast Schizosaccharomyces pombe
por: Li, Jun, et al.
Publicado: (2011)

Expression-independent gene trap vectors for random and targeted mutagenesis in embryonic stem cells
por: Tsakiridis, Anestis, et al.
Publicado: (2009)

A simple method using Pyrosequencing(TM) to identify de novo SNPs in pooled DNA samples
por: Lin, Yeong-Shin, et al.
Publicado: (2011)

Massive functional mapping of a 5′-UTR by saturation mutagenesis, phenotypic sorting and deep sequencing
por: Holmqvist, Erik, et al.
Publicado: (2013)

Site-specific terminal and internal labeling of RNA by poly(A) polymerase tailing and copper-catalyzed or copper-free strain-promoted click chemistry
por: Winz, Marie-Luise, et al.
Publicado: (2012)

Slingshot: a PiggyBac based transposon system for tamoxifen-inducible ‘self-inactivating’ insertional mutagenesis
por: Kong, Jun, et al.
Publicado: (2010)

A polymerase engineered for bisulfite sequencing
por: Millar, Doug, et al.
Publicado: (2015)

Plasmid-based lacZα assay for DNA polymerase fidelity: application to archaeal family-B DNA polymerase
por: Jozwiakowski, Stanislaw K., et al.
Publicado: (2009)

SAMURAI (Solid-phase Assisted Mutagenesis by Uracil Restriction for Accurate Integration) for antibody affinity maturation and paratope mapping
por: Hu, Francis Jingxin, et al.
Publicado: (2019)

Transmicron: accurate prediction of insertion probabilities improves detection of cancer driver genes from transposon mutagenesis screens
por: Bredthauer, Carl, et al.
Publicado: (2023)

Molecular breeding of polymerases for resistance to environmental inhibitors
por: Baar, Claudia, et al.
Publicado: (2011)

Dissecting and tuning primer editing by proofreading polymerases
por: Gohl, Daryl M, et al.
Publicado: (2021)

Collection of homozygous mutant mouse embryonic stem cells arising from autodiploidization during haploid gene trap mutagenesis
por: Yamanishi, Ayako, et al.
Publicado: (2018)

Development of a selection assay for small guide RNAs that drive efficient site-directed RNA editing
por: Diaz Quiroz, Juan Felipe, et al.
Publicado: (2023)

Mapping DNA polymerase errors by single-molecule sequencing
por: Lee, David F., et al.
Publicado: (2016)

A method for mutagenesis of mouse mtDNA and a resource of mouse mtDNA mutations for modeling human pathological conditions
por: Fayzulin, Rafik Z., et al.
Publicado: (2015)

Directed adenovirus evolution using engineered mutator viral polymerases
por: Uil, Taco G., et al.
Publicado: (2011)

Synthesis of 2′-Fluoro RNA by Syn5 RNA polymerase
por: Zhu, Bin, et al.
Publicado: (2015)

3′-End labeling of nucleic acids by a polymerase ribozyme
por: Samanta, Biswajit, et al.
Publicado: (2018)

SeqTar: an effective method for identifying microRNA guided cleavage sites from degradome of polyadenylated transcripts in plants
por: Zheng, Yun, et al.
Publicado: (2012)

Short-hairpin RNAs synthesized by T7 phage polymerase do not induce interferon
por: Gondai, Takuma, et al.
Publicado: (2008)

A real-time fluorescence method for enzymatic characterization of specialized human DNA polymerases
por: Dorjsuren, Dorjbal, et al.
Publicado: (2009)

High-precision mapping of protein–protein interfaces: an integrated genetic strategy combining en masse mutagenesis and DNA-level parallel analysis on a yeast two-hybrid platform
por: Pajunen, Maria, et al.
Publicado: (2007)

An artificial processivity clamp made with streptavidin facilitates oriented attachment of polymerase–DNA complexes to surfaces
por: Williams, John G. K., et al.
Publicado: (2008)

Cannot write session to /tmp/vufind_sessions/sess_ml4d0oomk2a7p69cev2hq965da