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New insights into the QuikChange(TM) process guide the use of Phusion DNA polymerase for site-directed mutagenesis

The QuikChange(TM) site-directed mutagenesis method is popular but imperfect. An improvement by using partially overlapping primers has been reported several times; however, it is incompatible with the proposed mechanism. The QuikChange(TM) method using complementary primers is proposed to linearly...

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Detalles Bibliográficos
Autores principales:	Xia, Yongzhen, Chu, Wenqiao, Qi, Qingsheng, Xun, Luying
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Oxford University Press 2015
Materias:	Methods Online
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4333370/ https://www.ncbi.nlm.nih.gov/pubmed/25399421 http://dx.doi.org/10.1093/nar/gku1189

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