Genetic Encoding of Photocaged Cysteine Allows Photoactivation of TEV Protease in Live Mammalian Cells

[Image: see text] We demonstrate the evolution of the PylRS/tRNA(CUA) pair for genetically encoding photocaged cysteine. By characterizing the incorporation in Escherichia coli and mammalian cells, and the photodeprotection process in vitro and in mammalian cells, we establish conditions for rapid e...

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Autores principales: Nguyen, Duy P., Mahesh, Mohan, Elsässer, Simon J., Hancock, Susan M., Uttamapinant, Chayasith, Chin, Jason W.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Chemical Society 2014
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4333589/
https://www.ncbi.nlm.nih.gov/pubmed/24479649
http://dx.doi.org/10.1021/ja412191m
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author Nguyen, Duy P.
Mahesh, Mohan
Elsässer, Simon J.
Hancock, Susan M.
Uttamapinant, Chayasith
Chin, Jason W.
author_facet Nguyen, Duy P.
Mahesh, Mohan
Elsässer, Simon J.
Hancock, Susan M.
Uttamapinant, Chayasith
Chin, Jason W.
author_sort Nguyen, Duy P.
collection PubMed
description [Image: see text] We demonstrate the evolution of the PylRS/tRNA(CUA) pair for genetically encoding photocaged cysteine. By characterizing the incorporation in Escherichia coli and mammalian cells, and the photodeprotection process in vitro and in mammalian cells, we establish conditions for rapid efficient photodeprotection to reveal native proteins in live cells. We demonstrate the utility of this approach by rapidly activating TEV protease following illumination of single cells.
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spelling pubmed-43335892015-02-19 Genetic Encoding of Photocaged Cysteine Allows Photoactivation of TEV Protease in Live Mammalian Cells Nguyen, Duy P. Mahesh, Mohan Elsässer, Simon J. Hancock, Susan M. Uttamapinant, Chayasith Chin, Jason W. J Am Chem Soc [Image: see text] We demonstrate the evolution of the PylRS/tRNA(CUA) pair for genetically encoding photocaged cysteine. By characterizing the incorporation in Escherichia coli and mammalian cells, and the photodeprotection process in vitro and in mammalian cells, we establish conditions for rapid efficient photodeprotection to reveal native proteins in live cells. We demonstrate the utility of this approach by rapidly activating TEV protease following illumination of single cells. American Chemical Society 2014-01-30 2014-02-12 /pmc/articles/PMC4333589/ /pubmed/24479649 http://dx.doi.org/10.1021/ja412191m Text en Copyright © 2014 American Chemical Society This is an open access article published under a Creative Commons Attribution (CC-BY) License (http://pubs.acs.org/page/policy/authorchoice_ccby_termsofuse.html) , which permits unrestricted use, distribution and reproduction in any medium, provided the author and source are cited.
spellingShingle Nguyen, Duy P.
Mahesh, Mohan
Elsässer, Simon J.
Hancock, Susan M.
Uttamapinant, Chayasith
Chin, Jason W.
Genetic Encoding of Photocaged Cysteine Allows Photoactivation of TEV Protease in Live Mammalian Cells
title Genetic Encoding of Photocaged Cysteine Allows Photoactivation of TEV Protease in Live Mammalian Cells
title_full Genetic Encoding of Photocaged Cysteine Allows Photoactivation of TEV Protease in Live Mammalian Cells
title_fullStr Genetic Encoding of Photocaged Cysteine Allows Photoactivation of TEV Protease in Live Mammalian Cells
title_full_unstemmed Genetic Encoding of Photocaged Cysteine Allows Photoactivation of TEV Protease in Live Mammalian Cells
title_short Genetic Encoding of Photocaged Cysteine Allows Photoactivation of TEV Protease in Live Mammalian Cells
title_sort genetic encoding of photocaged cysteine allows photoactivation of tev protease in live mammalian cells
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4333589/
https://www.ncbi.nlm.nih.gov/pubmed/24479649
http://dx.doi.org/10.1021/ja412191m
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