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Efficient Multisite Unnatural Amino Acid Incorporation in Mammalian Cells via Optimized Pyrrolysyl tRNA Synthetase/tRNA Expression and Engineered eRF1

[Image: see text] The efficient, site-specific introduction of unnatural amino acids into proteins in mammalian cells is an outstanding challenge in realizing the potential of genetic code expansion approaches. Addressing this challenge will allow the synthesis of modified recombinant proteins and a...

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Detalles Bibliográficos
Autores principales:	Schmied, Wolfgang H., Elsässer, Simon J., Uttamapinant, Chayasith, Chin, Jason W.
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	American Chemical Society 2014
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4333590/ https://www.ncbi.nlm.nih.gov/pubmed/25350841 http://dx.doi.org/10.1021/ja5069728

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