An infected chicken kidney cell co-culture ELISpot for enhanced detection of T cell responses to avian influenza and vaccination

A better understanding of the immune responses of chickens to the influenza virus is essential for the development of new strategies of vaccination and control. We have developed a method incorporating infected chicken kidney cells (CKC) in culture with splenocytes in an IFNγ ELISpot assay to enumer...

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Autores principales: Ruiz-Hernandez, Raul, Peroval, Marylene, Boyd, Amy, Balkissoon, Devanand, Staines, Karen, Smith, Adrian, Butter, Colin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2015
Materias:
Research Paper
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4334094/
https://www.ncbi.nlm.nih.gov/pubmed/25450002
http://dx.doi.org/10.1016/j.jim.2014.10.012
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author Ruiz-Hernandez, Raul
Peroval, Marylene
Boyd, Amy
Balkissoon, Devanand
Staines, Karen
Smith, Adrian
Butter, Colin
author_facet Ruiz-Hernandez, Raul
Peroval, Marylene
Boyd, Amy
Balkissoon, Devanand
Staines, Karen
Smith, Adrian
Butter, Colin
author_sort Ruiz-Hernandez, Raul
collection PubMed
description A better understanding of the immune responses of chickens to the influenza virus is essential for the development of new strategies of vaccination and control. We have developed a method incorporating infected chicken kidney cells (CKC) in culture with splenocytes in an IFNγ ELISpot assay to enumerate ex vivo responses against influenza virus antigens. Splenocytes from birds challenged with influenza showed specific responses to the influenza virus, with responding cells being mainly CD8 positive. The utility of the assay was also demonstrated in the detection of an antigen specific enhancement of IFNγ producing cells from birds vaccinated with recombinant Fowlpox vectored influenza nucleoprotein and matrix protein.
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spelling pubmed-43340942015-03-03 An infected chicken kidney cell co-culture ELISpot for enhanced detection of T cell responses to avian influenza and vaccination Ruiz-Hernandez, Raul Peroval, Marylene Boyd, Amy Balkissoon, Devanand Staines, Karen Smith, Adrian Butter, Colin J Immunol Methods Research Paper A better understanding of the immune responses of chickens to the influenza virus is essential for the development of new strategies of vaccination and control. We have developed a method incorporating infected chicken kidney cells (CKC) in culture with splenocytes in an IFNγ ELISpot assay to enumerate ex vivo responses against influenza virus antigens. Splenocytes from birds challenged with influenza showed specific responses to the influenza virus, with responding cells being mainly CD8 positive. The utility of the assay was also demonstrated in the detection of an antigen specific enhancement of IFNγ producing cells from birds vaccinated with recombinant Fowlpox vectored influenza nucleoprotein and matrix protein. Elsevier 2015-01 /pmc/articles/PMC4334094/ /pubmed/25450002 http://dx.doi.org/10.1016/j.jim.2014.10.012 Text en © 2014 The Authors http://creativecommons.org/licenses/by/3.0/ This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/3.0/).
spellingShingle Research Paper
Ruiz-Hernandez, Raul
Peroval, Marylene
Boyd, Amy
Balkissoon, Devanand
Staines, Karen
Smith, Adrian
Butter, Colin
An infected chicken kidney cell co-culture ELISpot for enhanced detection of T cell responses to avian influenza and vaccination
title An infected chicken kidney cell co-culture ELISpot for enhanced detection of T cell responses to avian influenza and vaccination
title_full An infected chicken kidney cell co-culture ELISpot for enhanced detection of T cell responses to avian influenza and vaccination
title_fullStr An infected chicken kidney cell co-culture ELISpot for enhanced detection of T cell responses to avian influenza and vaccination
title_full_unstemmed An infected chicken kidney cell co-culture ELISpot for enhanced detection of T cell responses to avian influenza and vaccination
title_short An infected chicken kidney cell co-culture ELISpot for enhanced detection of T cell responses to avian influenza and vaccination
title_sort infected chicken kidney cell co-culture elispot for enhanced detection of t cell responses to avian influenza and vaccination
topic Research Paper
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4334094/
https://www.ncbi.nlm.nih.gov/pubmed/25450002
http://dx.doi.org/10.1016/j.jim.2014.10.012
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