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An infected chicken kidney cell co-culture ELISpot for enhanced detection of T cell responses to avian influenza and vaccination
A better understanding of the immune responses of chickens to the influenza virus is essential for the development of new strategies of vaccination and control. We have developed a method incorporating infected chicken kidney cells (CKC) in culture with splenocytes in an IFNγ ELISpot assay to enumer...
Autores principales: | Ruiz-Hernandez, Raul, Peroval, Marylene, Boyd, Amy, Balkissoon, Devanand, Staines, Karen, Smith, Adrian, Butter, Colin |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier
2015
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4334094/ https://www.ncbi.nlm.nih.gov/pubmed/25450002 http://dx.doi.org/10.1016/j.jim.2014.10.012 |
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