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Comparison of three magnetic nanoparticle tracers for sentinel lymph node biopsy in an in vivo porcine model
INTRODUCTION: Breast cancer staging with sentinel lymph node biopsy relies on the use of radioisotopes, which limits the availability of the procedure worldwide. The use of a magnetic nanoparticle tracer and a handheld magnetometer provides a radiation-free alternative, which was recently evaluated...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Dove Medical Press
2015
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4334341/ https://www.ncbi.nlm.nih.gov/pubmed/25709445 http://dx.doi.org/10.2147/IJN.S76962 |
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author | Pouw, Joost J Ahmed, Muneer Anninga, Bauke Schuurman, Kimberley Pinder, Sarah E Van Hemelrijck, Mieke Pankhurst, Quentin A Douek, Michael ten Haken, Bennie |
author_facet | Pouw, Joost J Ahmed, Muneer Anninga, Bauke Schuurman, Kimberley Pinder, Sarah E Van Hemelrijck, Mieke Pankhurst, Quentin A Douek, Michael ten Haken, Bennie |
author_sort | Pouw, Joost J |
collection | PubMed |
description | INTRODUCTION: Breast cancer staging with sentinel lymph node biopsy relies on the use of radioisotopes, which limits the availability of the procedure worldwide. The use of a magnetic nanoparticle tracer and a handheld magnetometer provides a radiation-free alternative, which was recently evaluated in two clinical trials. The hydrodynamic particle size of the used magnetic tracer differs substantially from the radioisotope tracer and could therefore benefit from optimization. The aim of this study was to assess the performance of three different-sized magnetic nanoparticle tracers for sentinel lymph node biopsy within an in vivo porcine model. MATERIALS AND METHODS: Sentinel lymph node biopsy was performed within a validated porcine model using three magnetic nanoparticle tracers, approved for use in humans (ferumoxytol, with hydrodynamic diameter d(H) =32 nm; Sienna+(®), d(H) =59 nm; and ferumoxide, d(H) =111 nm), and a handheld magnetometer. Magnetometer counts (transcutaneous and ex vivo), iron quantification (vibrating sample magnetometry), and histopathological assessments were performed on all ex vivo nodes. RESULTS: Transcutaneous “hotspots” were present in 12/12 cases within 30 minutes of injection for the 59 nm tracer, compared to 7/12 for the 32 nm tracer and 8/12 for the 111 nm tracer, at the same time point. Ex vivo magnetometer counts were significantly greater for the 59 nm tracer than for the other tracers. Significantly more nodes per basin were excised for the 32 nm tracer compared to other tracers, indicating poor retention of the 32 nm tracer. Using the 59 nm tracer resulted in a significantly higher iron accumulation compared to the 32 nm tracer. CONCLUSION: The 59 nm tracer demonstrated rapid lymphatic uptake, retention in the first nodes reached, and accumulation in high concentration, making it the most suitable tracer for intraoperative sentinel lymph node localization. |
format | Online Article Text |
id | pubmed-4334341 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2015 |
publisher | Dove Medical Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-43343412015-02-23 Comparison of three magnetic nanoparticle tracers for sentinel lymph node biopsy in an in vivo porcine model Pouw, Joost J Ahmed, Muneer Anninga, Bauke Schuurman, Kimberley Pinder, Sarah E Van Hemelrijck, Mieke Pankhurst, Quentin A Douek, Michael ten Haken, Bennie Int J Nanomedicine Original Research INTRODUCTION: Breast cancer staging with sentinel lymph node biopsy relies on the use of radioisotopes, which limits the availability of the procedure worldwide. The use of a magnetic nanoparticle tracer and a handheld magnetometer provides a radiation-free alternative, which was recently evaluated in two clinical trials. The hydrodynamic particle size of the used magnetic tracer differs substantially from the radioisotope tracer and could therefore benefit from optimization. The aim of this study was to assess the performance of three different-sized magnetic nanoparticle tracers for sentinel lymph node biopsy within an in vivo porcine model. MATERIALS AND METHODS: Sentinel lymph node biopsy was performed within a validated porcine model using three magnetic nanoparticle tracers, approved for use in humans (ferumoxytol, with hydrodynamic diameter d(H) =32 nm; Sienna+(®), d(H) =59 nm; and ferumoxide, d(H) =111 nm), and a handheld magnetometer. Magnetometer counts (transcutaneous and ex vivo), iron quantification (vibrating sample magnetometry), and histopathological assessments were performed on all ex vivo nodes. RESULTS: Transcutaneous “hotspots” were present in 12/12 cases within 30 minutes of injection for the 59 nm tracer, compared to 7/12 for the 32 nm tracer and 8/12 for the 111 nm tracer, at the same time point. Ex vivo magnetometer counts were significantly greater for the 59 nm tracer than for the other tracers. Significantly more nodes per basin were excised for the 32 nm tracer compared to other tracers, indicating poor retention of the 32 nm tracer. Using the 59 nm tracer resulted in a significantly higher iron accumulation compared to the 32 nm tracer. CONCLUSION: The 59 nm tracer demonstrated rapid lymphatic uptake, retention in the first nodes reached, and accumulation in high concentration, making it the most suitable tracer for intraoperative sentinel lymph node localization. Dove Medical Press 2015-02-11 /pmc/articles/PMC4334341/ /pubmed/25709445 http://dx.doi.org/10.2147/IJN.S76962 Text en © 2015 Pouw et al. This work is published by Dove Medical Press Limited, and licensed under Creative Commons Attribution – Non Commercial (unported, v3.0) License The full terms of the License are available at http://creativecommons.org/licenses/by-nc/3.0/. Non-commercial uses of the work are permitted without any further permission from Dove Medical Press Limited, provided the work is properly attributed. |
spellingShingle | Original Research Pouw, Joost J Ahmed, Muneer Anninga, Bauke Schuurman, Kimberley Pinder, Sarah E Van Hemelrijck, Mieke Pankhurst, Quentin A Douek, Michael ten Haken, Bennie Comparison of three magnetic nanoparticle tracers for sentinel lymph node biopsy in an in vivo porcine model |
title | Comparison of three magnetic nanoparticle tracers for sentinel lymph node biopsy in an in vivo porcine model |
title_full | Comparison of three magnetic nanoparticle tracers for sentinel lymph node biopsy in an in vivo porcine model |
title_fullStr | Comparison of three magnetic nanoparticle tracers for sentinel lymph node biopsy in an in vivo porcine model |
title_full_unstemmed | Comparison of three magnetic nanoparticle tracers for sentinel lymph node biopsy in an in vivo porcine model |
title_short | Comparison of three magnetic nanoparticle tracers for sentinel lymph node biopsy in an in vivo porcine model |
title_sort | comparison of three magnetic nanoparticle tracers for sentinel lymph node biopsy in an in vivo porcine model |
topic | Original Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4334341/ https://www.ncbi.nlm.nih.gov/pubmed/25709445 http://dx.doi.org/10.2147/IJN.S76962 |
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