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Multi Locus Variable-Number Tandem Repeat (MLVA) Typing Tools Improved the Surveillance of Salmonella Enteritidis: A 6 Years Retrospective Study

Surveillance of Salmonella enterica subsp. enterica serovar Enteritidis is generally considered to benefit from molecular techniques like multiple-locus variable-number of tandem repeats analysis (MLVA), which allow early detection and confinement of outbreaks. Here, a surveillance study, including...

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Autores principales: Bertrand, Sophie, De Lamine de Bex, Guillaume, Wildemauwe, Christa, Lunguya, Octavie, Phoba, Marie France, Ley, Benedikt, Jacobs, Jan, Vanhoof, Raymond, Mattheus, Wesley
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4334661/
https://www.ncbi.nlm.nih.gov/pubmed/25693200
http://dx.doi.org/10.1371/journal.pone.0117950
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author Bertrand, Sophie
De Lamine de Bex, Guillaume
Wildemauwe, Christa
Lunguya, Octavie
Phoba, Marie France
Ley, Benedikt
Jacobs, Jan
Vanhoof, Raymond
Mattheus, Wesley
author_facet Bertrand, Sophie
De Lamine de Bex, Guillaume
Wildemauwe, Christa
Lunguya, Octavie
Phoba, Marie France
Ley, Benedikt
Jacobs, Jan
Vanhoof, Raymond
Mattheus, Wesley
author_sort Bertrand, Sophie
collection PubMed
description Surveillance of Salmonella enterica subsp. enterica serovar Enteritidis is generally considered to benefit from molecular techniques like multiple-locus variable-number of tandem repeats analysis (MLVA), which allow early detection and confinement of outbreaks. Here, a surveillance study, including phage typing, antimicrobial susceptibility testing and MLVA on 1,535 S. Enteritidis isolates collected between 2007 and 2012, was used to evaluate the added value of MLVA for public health surveillance in Belgium. Phage types PT4, PT8, PT21, PT1, PT6, PT14b, PT28 and PT13 dominate the Belgian S. Enteritidis population. The isolates of S. Enteritidis were most frequently susceptible to all antibiotics tested. 172 different MLVA profiles were detected, of which 9 frequent profiles included 67.2% of the S. Enteritidis population. During a serial passage experiment on selected isolates to investigate the in vitro stability of the 5 MLVA loci, no variations over time were observed indicating that the MLVA profiles were stable. The MLVA profile of isolates originating from different outbreaks in the Democratic Republic of the Congo (DRC) between 2010 and 2011 were distinct from any of the MLVA profiles found in Belgian isolates throughout the six year observational period and demonstrates that MLVA improves public health surveillance of S. Enteritidis. However, MLVA should be complemented with other subtyping methods when investigating outbreaks is caused by the most common MLVA profile.
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spelling pubmed-43346612015-02-24 Multi Locus Variable-Number Tandem Repeat (MLVA) Typing Tools Improved the Surveillance of Salmonella Enteritidis: A 6 Years Retrospective Study Bertrand, Sophie De Lamine de Bex, Guillaume Wildemauwe, Christa Lunguya, Octavie Phoba, Marie France Ley, Benedikt Jacobs, Jan Vanhoof, Raymond Mattheus, Wesley PLoS One Research Article Surveillance of Salmonella enterica subsp. enterica serovar Enteritidis is generally considered to benefit from molecular techniques like multiple-locus variable-number of tandem repeats analysis (MLVA), which allow early detection and confinement of outbreaks. Here, a surveillance study, including phage typing, antimicrobial susceptibility testing and MLVA on 1,535 S. Enteritidis isolates collected between 2007 and 2012, was used to evaluate the added value of MLVA for public health surveillance in Belgium. Phage types PT4, PT8, PT21, PT1, PT6, PT14b, PT28 and PT13 dominate the Belgian S. Enteritidis population. The isolates of S. Enteritidis were most frequently susceptible to all antibiotics tested. 172 different MLVA profiles were detected, of which 9 frequent profiles included 67.2% of the S. Enteritidis population. During a serial passage experiment on selected isolates to investigate the in vitro stability of the 5 MLVA loci, no variations over time were observed indicating that the MLVA profiles were stable. The MLVA profile of isolates originating from different outbreaks in the Democratic Republic of the Congo (DRC) between 2010 and 2011 were distinct from any of the MLVA profiles found in Belgian isolates throughout the six year observational period and demonstrates that MLVA improves public health surveillance of S. Enteritidis. However, MLVA should be complemented with other subtyping methods when investigating outbreaks is caused by the most common MLVA profile. Public Library of Science 2015-02-18 /pmc/articles/PMC4334661/ /pubmed/25693200 http://dx.doi.org/10.1371/journal.pone.0117950 Text en © 2015 Bertrand et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Bertrand, Sophie
De Lamine de Bex, Guillaume
Wildemauwe, Christa
Lunguya, Octavie
Phoba, Marie France
Ley, Benedikt
Jacobs, Jan
Vanhoof, Raymond
Mattheus, Wesley
Multi Locus Variable-Number Tandem Repeat (MLVA) Typing Tools Improved the Surveillance of Salmonella Enteritidis: A 6 Years Retrospective Study
title Multi Locus Variable-Number Tandem Repeat (MLVA) Typing Tools Improved the Surveillance of Salmonella Enteritidis: A 6 Years Retrospective Study
title_full Multi Locus Variable-Number Tandem Repeat (MLVA) Typing Tools Improved the Surveillance of Salmonella Enteritidis: A 6 Years Retrospective Study
title_fullStr Multi Locus Variable-Number Tandem Repeat (MLVA) Typing Tools Improved the Surveillance of Salmonella Enteritidis: A 6 Years Retrospective Study
title_full_unstemmed Multi Locus Variable-Number Tandem Repeat (MLVA) Typing Tools Improved the Surveillance of Salmonella Enteritidis: A 6 Years Retrospective Study
title_short Multi Locus Variable-Number Tandem Repeat (MLVA) Typing Tools Improved the Surveillance of Salmonella Enteritidis: A 6 Years Retrospective Study
title_sort multi locus variable-number tandem repeat (mlva) typing tools improved the surveillance of salmonella enteritidis: a 6 years retrospective study
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4334661/
https://www.ncbi.nlm.nih.gov/pubmed/25693200
http://dx.doi.org/10.1371/journal.pone.0117950
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