Determination of Sinomenine in Cubosome Nanoparticles by HPLC Technique

We applied HPLC technique to quantitatively analyze sinomenine in cubosome nanoparticles. The chromatographic method was performed by using an isocratic system. The mobile phase was composed of methanol-PBS(pH6.8)-triethylamine (50 : 50 : 0.1%) with a flow rate of 1 mL/min; the detection wavelength...

Descripción completa

Detalles Bibliográficos
Autores principales: Zhou, Yanfang, Guo, Chunlian, Chen, Hongying, Zhang, Yudai, Peng, Xinsheng, Zhu, Ping
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi Publishing Corporation 2015
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4334977/
https://www.ncbi.nlm.nih.gov/pubmed/25734024
http://dx.doi.org/10.1155/2015/931687
_version_ 1782358264488394752
author Zhou, Yanfang
Guo, Chunlian
Chen, Hongying
Zhang, Yudai
Peng, Xinsheng
Zhu, Ping
author_facet Zhou, Yanfang
Guo, Chunlian
Chen, Hongying
Zhang, Yudai
Peng, Xinsheng
Zhu, Ping
author_sort Zhou, Yanfang
collection PubMed
description We applied HPLC technique to quantitatively analyze sinomenine in cubosome nanoparticles. The chromatographic method was performed by using an isocratic system. The mobile phase was composed of methanol-PBS(pH6.8)-triethylamine (50 : 50 : 0.1%) with a flow rate of 1 mL/min; the detection wavelength was at 265 nm. Sinomenine can be successfully separated with good linearity (the regression equation is A = 10835C + 1058; R (2) = 1.0) and perfect recovery (102.2%). The chromatograph technique was proper for quality control of sinomenine in cubosome nanoparticles.
format Online
Article
Text
id pubmed-4334977
institution National Center for Biotechnology Information
language English
publishDate 2015
publisher Hindawi Publishing Corporation
record_format MEDLINE/PubMed
spelling pubmed-43349772015-03-02 Determination of Sinomenine in Cubosome Nanoparticles by HPLC Technique Zhou, Yanfang Guo, Chunlian Chen, Hongying Zhang, Yudai Peng, Xinsheng Zhu, Ping J Anal Methods Chem Research Article We applied HPLC technique to quantitatively analyze sinomenine in cubosome nanoparticles. The chromatographic method was performed by using an isocratic system. The mobile phase was composed of methanol-PBS(pH6.8)-triethylamine (50 : 50 : 0.1%) with a flow rate of 1 mL/min; the detection wavelength was at 265 nm. Sinomenine can be successfully separated with good linearity (the regression equation is A = 10835C + 1058; R (2) = 1.0) and perfect recovery (102.2%). The chromatograph technique was proper for quality control of sinomenine in cubosome nanoparticles. Hindawi Publishing Corporation 2015 2015-02-05 /pmc/articles/PMC4334977/ /pubmed/25734024 http://dx.doi.org/10.1155/2015/931687 Text en Copyright © 2015 Yanfang Zhou et al. https://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Zhou, Yanfang
Guo, Chunlian
Chen, Hongying
Zhang, Yudai
Peng, Xinsheng
Zhu, Ping
Determination of Sinomenine in Cubosome Nanoparticles by HPLC Technique
title Determination of Sinomenine in Cubosome Nanoparticles by HPLC Technique
title_full Determination of Sinomenine in Cubosome Nanoparticles by HPLC Technique
title_fullStr Determination of Sinomenine in Cubosome Nanoparticles by HPLC Technique
title_full_unstemmed Determination of Sinomenine in Cubosome Nanoparticles by HPLC Technique
title_short Determination of Sinomenine in Cubosome Nanoparticles by HPLC Technique
title_sort determination of sinomenine in cubosome nanoparticles by hplc technique
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4334977/
https://www.ncbi.nlm.nih.gov/pubmed/25734024
http://dx.doi.org/10.1155/2015/931687
work_keys_str_mv AT zhouyanfang determinationofsinomenineincubosomenanoparticlesbyhplctechnique
AT guochunlian determinationofsinomenineincubosomenanoparticlesbyhplctechnique
AT chenhongying determinationofsinomenineincubosomenanoparticlesbyhplctechnique
AT zhangyudai determinationofsinomenineincubosomenanoparticlesbyhplctechnique
AT pengxinsheng determinationofsinomenineincubosomenanoparticlesbyhplctechnique
AT zhuping determinationofsinomenineincubosomenanoparticlesbyhplctechnique

Ejemplares similares