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Quantification of Sunscreen Benzophenone-4 in Hair Shampoos by Hydrophilic Interactions Thin-Layer Chromatography/Densitometry or Derivative UV Spectrophotometry

Benzophenone-4 (BZ4) was separated from surfactants, dyes, preservatives, and other components of hair shampoos by thin-layer chromatography on silica gel 60 stationary phase, with ethyl acetate-ethanol-water-pH 6 phosphate buffer (15 : 7 : 5 : 1 v/v/v/v) as mobile phase. Densitometry scanning of ch...

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Detalles Bibliográficos
Autores principales: Sobańska, Anna W., Kałębasiak, Katarzyna, Pyzowski, Jarosław, Brzezińska, Elżbieta
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi Publishing Corporation 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4334985/
https://www.ncbi.nlm.nih.gov/pubmed/25734022
http://dx.doi.org/10.1155/2015/695658
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author Sobańska, Anna W.
Kałębasiak, Katarzyna
Pyzowski, Jarosław
Brzezińska, Elżbieta
author_facet Sobańska, Anna W.
Kałębasiak, Katarzyna
Pyzowski, Jarosław
Brzezińska, Elżbieta
author_sort Sobańska, Anna W.
collection PubMed
description Benzophenone-4 (BZ4) was separated from surfactants, dyes, preservatives, and other components of hair shampoos by thin-layer chromatography on silica gel 60 stationary phase, with ethyl acetate-ethanol-water-pH 6 phosphate buffer (15 : 7 : 5 : 1 v/v/v/v) as mobile phase. Densitometry scanning of chromatograms was performed at 285 nm. The densitometric calibration curve for BZ4 was nonlinear (second-degree polynomial), with R > 0.999. The limits of detection and quantification were ca. 0.03 and ca. 0.1 μg spot(−1), respectively. The results obtained by HPTLC-densitometry were compared to those obtained by zero and 2nd derivative UV spectrophotometry. In the case of spectrophotometric methods, calibration curves were linear with R > 0.9998. The chromatographic method was fully validated.
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spelling pubmed-43349852015-03-02 Quantification of Sunscreen Benzophenone-4 in Hair Shampoos by Hydrophilic Interactions Thin-Layer Chromatography/Densitometry or Derivative UV Spectrophotometry Sobańska, Anna W. Kałębasiak, Katarzyna Pyzowski, Jarosław Brzezińska, Elżbieta J Anal Methods Chem Research Article Benzophenone-4 (BZ4) was separated from surfactants, dyes, preservatives, and other components of hair shampoos by thin-layer chromatography on silica gel 60 stationary phase, with ethyl acetate-ethanol-water-pH 6 phosphate buffer (15 : 7 : 5 : 1 v/v/v/v) as mobile phase. Densitometry scanning of chromatograms was performed at 285 nm. The densitometric calibration curve for BZ4 was nonlinear (second-degree polynomial), with R > 0.999. The limits of detection and quantification were ca. 0.03 and ca. 0.1 μg spot(−1), respectively. The results obtained by HPTLC-densitometry were compared to those obtained by zero and 2nd derivative UV spectrophotometry. In the case of spectrophotometric methods, calibration curves were linear with R > 0.9998. The chromatographic method was fully validated. Hindawi Publishing Corporation 2015 2015-02-04 /pmc/articles/PMC4334985/ /pubmed/25734022 http://dx.doi.org/10.1155/2015/695658 Text en Copyright © 2015 Anna W. Sobańska et al. https://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Sobańska, Anna W.
Kałębasiak, Katarzyna
Pyzowski, Jarosław
Brzezińska, Elżbieta
Quantification of Sunscreen Benzophenone-4 in Hair Shampoos by Hydrophilic Interactions Thin-Layer Chromatography/Densitometry or Derivative UV Spectrophotometry
title Quantification of Sunscreen Benzophenone-4 in Hair Shampoos by Hydrophilic Interactions Thin-Layer Chromatography/Densitometry or Derivative UV Spectrophotometry
title_full Quantification of Sunscreen Benzophenone-4 in Hair Shampoos by Hydrophilic Interactions Thin-Layer Chromatography/Densitometry or Derivative UV Spectrophotometry
title_fullStr Quantification of Sunscreen Benzophenone-4 in Hair Shampoos by Hydrophilic Interactions Thin-Layer Chromatography/Densitometry or Derivative UV Spectrophotometry
title_full_unstemmed Quantification of Sunscreen Benzophenone-4 in Hair Shampoos by Hydrophilic Interactions Thin-Layer Chromatography/Densitometry or Derivative UV Spectrophotometry
title_short Quantification of Sunscreen Benzophenone-4 in Hair Shampoos by Hydrophilic Interactions Thin-Layer Chromatography/Densitometry or Derivative UV Spectrophotometry
title_sort quantification of sunscreen benzophenone-4 in hair shampoos by hydrophilic interactions thin-layer chromatography/densitometry or derivative uv spectrophotometry
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4334985/
https://www.ncbi.nlm.nih.gov/pubmed/25734022
http://dx.doi.org/10.1155/2015/695658
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