Cargando…
Quantification of Sunscreen Benzophenone-4 in Hair Shampoos by Hydrophilic Interactions Thin-Layer Chromatography/Densitometry or Derivative UV Spectrophotometry
Benzophenone-4 (BZ4) was separated from surfactants, dyes, preservatives, and other components of hair shampoos by thin-layer chromatography on silica gel 60 stationary phase, with ethyl acetate-ethanol-water-pH 6 phosphate buffer (15 : 7 : 5 : 1 v/v/v/v) as mobile phase. Densitometry scanning of ch...
Autores principales: | , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Hindawi Publishing Corporation
2015
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4334985/ https://www.ncbi.nlm.nih.gov/pubmed/25734022 http://dx.doi.org/10.1155/2015/695658 |
_version_ | 1782358266299285504 |
---|---|
author | Sobańska, Anna W. Kałębasiak, Katarzyna Pyzowski, Jarosław Brzezińska, Elżbieta |
author_facet | Sobańska, Anna W. Kałębasiak, Katarzyna Pyzowski, Jarosław Brzezińska, Elżbieta |
author_sort | Sobańska, Anna W. |
collection | PubMed |
description | Benzophenone-4 (BZ4) was separated from surfactants, dyes, preservatives, and other components of hair shampoos by thin-layer chromatography on silica gel 60 stationary phase, with ethyl acetate-ethanol-water-pH 6 phosphate buffer (15 : 7 : 5 : 1 v/v/v/v) as mobile phase. Densitometry scanning of chromatograms was performed at 285 nm. The densitometric calibration curve for BZ4 was nonlinear (second-degree polynomial), with R > 0.999. The limits of detection and quantification were ca. 0.03 and ca. 0.1 μg spot(−1), respectively. The results obtained by HPTLC-densitometry were compared to those obtained by zero and 2nd derivative UV spectrophotometry. In the case of spectrophotometric methods, calibration curves were linear with R > 0.9998. The chromatographic method was fully validated. |
format | Online Article Text |
id | pubmed-4334985 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2015 |
publisher | Hindawi Publishing Corporation |
record_format | MEDLINE/PubMed |
spelling | pubmed-43349852015-03-02 Quantification of Sunscreen Benzophenone-4 in Hair Shampoos by Hydrophilic Interactions Thin-Layer Chromatography/Densitometry or Derivative UV Spectrophotometry Sobańska, Anna W. Kałębasiak, Katarzyna Pyzowski, Jarosław Brzezińska, Elżbieta J Anal Methods Chem Research Article Benzophenone-4 (BZ4) was separated from surfactants, dyes, preservatives, and other components of hair shampoos by thin-layer chromatography on silica gel 60 stationary phase, with ethyl acetate-ethanol-water-pH 6 phosphate buffer (15 : 7 : 5 : 1 v/v/v/v) as mobile phase. Densitometry scanning of chromatograms was performed at 285 nm. The densitometric calibration curve for BZ4 was nonlinear (second-degree polynomial), with R > 0.999. The limits of detection and quantification were ca. 0.03 and ca. 0.1 μg spot(−1), respectively. The results obtained by HPTLC-densitometry were compared to those obtained by zero and 2nd derivative UV spectrophotometry. In the case of spectrophotometric methods, calibration curves were linear with R > 0.9998. The chromatographic method was fully validated. Hindawi Publishing Corporation 2015 2015-02-04 /pmc/articles/PMC4334985/ /pubmed/25734022 http://dx.doi.org/10.1155/2015/695658 Text en Copyright © 2015 Anna W. Sobańska et al. https://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Article Sobańska, Anna W. Kałębasiak, Katarzyna Pyzowski, Jarosław Brzezińska, Elżbieta Quantification of Sunscreen Benzophenone-4 in Hair Shampoos by Hydrophilic Interactions Thin-Layer Chromatography/Densitometry or Derivative UV Spectrophotometry |
title | Quantification of Sunscreen Benzophenone-4 in Hair Shampoos by Hydrophilic Interactions Thin-Layer Chromatography/Densitometry or Derivative UV Spectrophotometry |
title_full | Quantification of Sunscreen Benzophenone-4 in Hair Shampoos by Hydrophilic Interactions Thin-Layer Chromatography/Densitometry or Derivative UV Spectrophotometry |
title_fullStr | Quantification of Sunscreen Benzophenone-4 in Hair Shampoos by Hydrophilic Interactions Thin-Layer Chromatography/Densitometry or Derivative UV Spectrophotometry |
title_full_unstemmed | Quantification of Sunscreen Benzophenone-4 in Hair Shampoos by Hydrophilic Interactions Thin-Layer Chromatography/Densitometry or Derivative UV Spectrophotometry |
title_short | Quantification of Sunscreen Benzophenone-4 in Hair Shampoos by Hydrophilic Interactions Thin-Layer Chromatography/Densitometry or Derivative UV Spectrophotometry |
title_sort | quantification of sunscreen benzophenone-4 in hair shampoos by hydrophilic interactions thin-layer chromatography/densitometry or derivative uv spectrophotometry |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4334985/ https://www.ncbi.nlm.nih.gov/pubmed/25734022 http://dx.doi.org/10.1155/2015/695658 |
work_keys_str_mv | AT sobanskaannaw quantificationofsunscreenbenzophenone4inhairshampoosbyhydrophilicinteractionsthinlayerchromatographydensitometryorderivativeuvspectrophotometry AT kałebasiakkatarzyna quantificationofsunscreenbenzophenone4inhairshampoosbyhydrophilicinteractionsthinlayerchromatographydensitometryorderivativeuvspectrophotometry AT pyzowskijarosław quantificationofsunscreenbenzophenone4inhairshampoosbyhydrophilicinteractionsthinlayerchromatographydensitometryorderivativeuvspectrophotometry AT brzezinskaelzbieta quantificationofsunscreenbenzophenone4inhairshampoosbyhydrophilicinteractionsthinlayerchromatographydensitometryorderivativeuvspectrophotometry |